Published May 15, 1986 | Version v1
Journal article Metadata-only

Measurements of serum glucose using the luciferin/Luciferase system and a liquid scintillation spectrometer

Creators

  • 1. Univ. of Umea, Sweden

Description

A single-step assay for serum glucose measurements is described. The assay is based on the phosphorylation of D-glucose by glucokinase and the measurement of ATP consumption by firefly luciferase. The luminescence is recorded in an ordinary liquid scintillation spectrometer. The use of stable reagents and a stable final signal (light emission) makes it possible to analyze a large number of samples in each assay run. The assay is of particular value when repeated serum glucose determinations are performed on samples from small laboratory animals

Additional details

Publishing Information

Journal Title
Anal. Biochem.
Journal Volume
155
Journal Issue
1
Series
Anal. Biochem.
Journal Page Range
177-181
ISSN
0003-2697
CODEN
ANBCA

INIS

Country of Publication
United States
Country of Input or Organization
United States
INIS RN
17081492
Subject category
S37: INORGANIC, ORGANIC, PHYSICAL AND ANALYTICAL CHEMISTRY; S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ATP; BIOLUMINESCENCE; BLOOD SERUM; GLUCOSE; LIQUID SCINTILLATORS; LUCIFERASE; LUCIFERIN; PHOSPHORYLATION; PHOSPHOTRANSFERASES; QUANTITATIVE CHEMICAL ANALYSIS; SPECTROMETERS
Descriptors DEC
ALBUMINS; ALDEHYDES; BIOLOGICAL MATERIALS; BLOOD; BLOOD PLASMA; BODY FLUIDS; CARBOHYDRATES; CHEMICAL ANALYSIS; CHEMICAL REACTIONS; CHEMILUMINESCENCE; EMISSION; ENZYMES; HEXOSES; LUMINESCENCE; MATERIALS; MEASURING INSTRUMENTS; MONOSACCHARIDES; NUCLEOTIDES; ORGANIC COMPOUNDS; OXIDASES; OXIDOREDUCTASES; PHOSPHORS; PHOTON EMISSION; PROTEINS; SACCHARIDES; TRANSFERASES