Prostaglandin induced radioprotection in murine bone marrow stem cells and CHO cells

High concentrations of prostaglandins (PG) have been associated with a variety of diseases. However, a previous study reported an intestinal cytoprotective effect of PG when given before ulcerogenic treatment of indomethacin or absolute alcohol. Subsequently, another study showed that 16-16dm PGE/sub 2/ protected intestinal stem cells in mice if given before radiation. The shoulder and D/sub o/ of the survival curve was shifted to higher values in PG treated animals. To test whether 16-16dm PGE/sub 2/ induced radioprotection was unique to the intestine, survival of CFU-S was assayed in irradiated mice and compared to mice given the PG 1 hr before /sup 60/Co γ irradiation. To assess In Vitro PG induced radioprotection, cell survival was measured in CHO cell cultures irradiated only or after a 2 hr incubation with the PG. The 16-16dm PGE/sub 2/ protected CFU-S similarly to intestinal stem cells. The shoulder was significantly increased from 1.0 to 1.4 and the D/sub o/ was increased from 0.92 to 1.14 Gy. However there was no PG induced radioprotection in CHO cells confirming results using V-79 cells. These results show that PG induced radioprotection is not unique to intestinal stem cells, however, a difference apparently exists comparing radioprotection In Vivo and In Vitro