Published November 2006 | Version v1
Journal article Metadata-only

Detecting the effects of glutamate toxicity on influxing calcium in PC12 cells by AMS

Creators

  • 1. Institute of Microbiology and Epidemiology, Academy of Military Medical Science, Beijing (China)
  • 2. China Inst. of Atomic Energy, Beijing (China). Dept. of Nuclear Physics
  • 3. Shanghai Jiaotong Univ., Shanghai (China). Dept. of Physics
  • 4. Beijing Normal Univ., Beijing (China). College of Life Sciences

Description

The PC12 cells were traced by 41Ca and the final biological sample, CaF2, was measured by Accelerator Mass Spectrometry (AMS). The results were analyzed from two aspects: the time effect of glutamate on free calcium in PC12 cells; and the blocking effect of antagonist, MK-801, on receptor operated channel (ROC). Comparing with the results detected by laser scanning confocal microscope (LSCM), the results show that 41Ca AMS measurement can be used for detecting the change of calcium influx in PC12 cells effectively in different situations. (authors)

Additional details

Publishing Information

Journal Title
Nuclear Techniques
Journal Volume
29
Journal Issue
11
Journal Page Range
p. 821-825
ISSN
0253-3219

INIS

Country of Publication
China
Country of Input or Organization
China
INIS RN
38080029
Subject category
S60: APPLIED LIFE SCIENCES;
Descriptors DEI
ACCELERATORS; CALCIUM 41; CALCIUM FLUORIDES; CELL CULTURES; FOCUSONS; GLUTAMIC ACID; GLUTAMINE; LASER RADIATION; MASS SPECTROSCOPY; RECEPTORS; SCANNING LIGHT MICROSCOPY; TOXICITY; TUMOR CELLS
Descriptors DEC
ALKALINE EARTH ISOTOPES; ALKALINE EARTH METAL COMPOUNDS; AMIDES; AMINO ACIDS; ANIMAL CELLS; BETA DECAY RADIOISOTOPES; CALCIUM COMPOUNDS; CALCIUM HALIDES; CALCIUM ISOTOPES; CARBOXYLIC ACIDS; ELECTROMAGNETIC RADIATION; ELECTRON CAPTURE RADIOISOTOPES; EVEN-ODD NUCLEI; FLUORIDES; FLUORINE COMPOUNDS; HALIDES; HALOGEN COMPOUNDS; INTERMEDIATE MASS NUCLEI; ISOTOPES; MEMBRANE PROTEINS; MICROSCOPY; NUCLEI; OPTICAL MICROSCOPY; ORGANIC ACIDS; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; PROTEINS; QUASI PARTICLES; RADIATIONS; RADIOISOTOPES; SPECTROSCOPY; YEARS LIVING RADIOISOTOPES

Optional Information

Notes
3 figs., 2 tabs., 13 refs.