[en] The ¹H-NMR spectrum of MeSiCp determined at various temperatures is reported and discussed in terms of metallotropic and phototropic rearrangements. (author)

[en] Published in summary form only

[en] Functional interaction between Drosophila orphan receptor FTZ-F1 (NR5A3) and a segmentation gene product fushi tarazu (FTZ) is crucial for regulating genes related to define the identities of alternate segmental regions in the Drosophila embryo. FTZ binding to the ligand-binding domain (LBD) of FTZ-F1 is of essence in activating its transcription process. We determined solution structures of the cofactor peptide (FTZ^PEP) derived from FTZ by NMR spectroscopy. The cofactor peptide showed a nascent helical conformation in aqueous solution, however, the helicity was increased in the presence of TFE. Furthermore, FTZ^PEP formed α- helical conformation upon FTZ-F1 binding, which provides a receptor bound structure of FTZ^PEP. The solution structure of FTZ^PEP in the presence of FTZ-F1 displays a long stretch of the α-helix with a bend in the middle of helix

[en] Volume 10 of Annual Reports on NMR Spectroscopy consists of accounts covering three main areas of molecular science: NMR of living systems is covered for the first time in this series; various aspects of Boron NMR are dealt with in two, largely complementary chapters; the third area reported on is that of NMR studies of alkali anions in nonaqueous solvents

[en] Oximation of 20-hydroxyecdysone oxo derivatives proceeds regio- and stereoselectively and provides (E)-configured oximes in quantitative yields. The Ni-Ra-catalyzed hydrogenation of the oximes affords novel α-aminoecdysteroids with the unchanged 14α-hydroxy-7-en-6-on native chromophore of ecdysteroids. The structures of synthesized compounds were confirmed by the means of homo- and heteronuclear one-dimensional and two-dimensional ¹H and ¹³C NMR spectroscopy and X-ray crystallography. (author)

[en] A method for five-dimensional spectral reconstruction of non-uniformly sampled NMR data sets is proposed. It is derived from the previously published signal separation algorithm, with major alterations to avoid unfeasible processing of an entire five-dimensional spectrum. The proposed method allows credible reconstruction of spectra from as little as a few hundred data points and enables sensitive resonance detection in experiments with a high dynamic range of peak intensities. The efficiency of the method is demonstrated on two high-resolution spectra for rapid sequential assignment of intrinsically disordered proteins, namely 5D HN(CA)CONH and 5D (HACA)CON(CO)CONH.

[en] Functional interaction between Drosophila orphan receptor FTZ-F1 (NR5A3) and a segmentation gene product fushi tarazu (FTZ) is crucial for regulating genes related to define the identities of alternate segmental regions in the Drosophila embryo. FTZ binding to the ligand-binding domain (LBD) of FTZ-F1 is of essence in activating its transcription process. We determined solution structures of the cofactor peptide (FTZ^PEP) derived from FTZ by NMR spectroscopy. The cofactor peptide showed a nascent helical conformation in aqueous solution, however, the helicity was increased in the presence of TFE. Furthermore, FTZ^PEP formed α- helical conformation upon FTZ-F1 binding, which provides a receptor bound structure of FTZ^PEP. The solution structure of FTZ^PEP in the presence of FTZ-F1 displays a long stretch of the α-helix with a bend in the middle of helix

No abstract available

[en] Published in summary form only

[en] An arylpiperazine, WAY100635, has been found as a highly selective ligand for imaging 5-HT_1A receptor among other serotonergic receptors. We have established an efficient method for the synthesis of the arylpiperazine derivatives in which the acylation of 2-aminopyridine, the coupling reaction of the acyl compound with piperazines, and reduction of the arylpiperazines were performed under microwave irradiation (300 W) to afford the corresponding target compounds in quantitative yields. The synthesis time of the acylation, the coupling reaction and the reduction was remarkably reduced compared with that of the conventional method