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[en] mRNAs inside cells interact with numerous RNA-binding proteins, microRNAs, and ribosomes that together compose a highly heterogeneous population of messenger ribonucleoprotein (mRNP) particles. Perhaps one of the best ways to investigate the complex regulation of mRNA is to observe individual molecules. Single molecule imaging allows the collection of quantitative and statistical data on subpopulations and transient states that are otherwise obscured by ensemble averaging. In addition, single particle tracking reveals the sequence of events that occur in the formation and remodeling of mRNPs in real time. Here, we review the current state-of-the-art techniques in tagging, delivery, and imaging to track single mRNAs in live cells. We also discuss how these techniques are applied to extract dynamic information on the transcription, transport, localization, and translation of mRNAs. These studies demonstrate how single molecule tracking is transforming the understanding of mRNA regulation in live cells. (topical review)
[en] The article presents the results of analysis of statistical regularities (average seasonal and annual variations) of the changes in the total ozone, nitrogen oxide and water vapor in atmosphere, which were obtained during measurements at the AERONET station. It is shown that the dynamics of investigated greenhouse gases strongly depends on conditions of their generation and runoff, including meteorological and anthropogenic factors. (author)
[en] The article presents the results of analysis of statistical regularities (average daily and monthly variations) of changes in total ozone, nitrogen oxide and water vapor in the atmosphere, which were obtained during measurements at AERONET station. It is shown that dynamics of investigated greenhouse gases strongly depends on conditions of their generation and runoff, including meteorological and anthropogenic factors.
[en] The repeated running of standard 2006 by the Atomic Energy Board using the delayed neutron counting method has allowed the precision of this analytical process to be calculated. Standard 2006 was run 156 times giving an average U content of 5.96 ppm and a standard deviation of .28 ppm. The precision of this analytical method is good if the low concentration levels of U are considered
[en] This paper discusses the preliminary results of optical and micro physical properties of the atmosphere in Dushanbe, conducted between July 2010 till March 2011 using the sun photometer C E-318 on framework of AERONET program. Aerosol optical thickness decreases monotonically with increasing wavelength. The analysis of seasonal changes in dust associated with the intrusion of dust haze. (author)