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[en] The effect of the ionic contrast media diatrizoate, iocarmate and metrizoate and the non-ionic metrizamide on whole blood viscosity, plasma viscosity and hematocrit was investigated. All the contrast media increased whole blood and plasma viscosity and reduced the hematocrit. The whole blood viscosity increased with increasing osmolality of the contrast medium solutions, whereas the plasma viscosity increased with increasing viscosity of the contrast medium solutions. The higher the osmolality of the contrast media, the lower the hematocrit became. The normal shear-thinning (decreasing viscosity with increasing shear rate) property of blood was reduced when contrast medium was added to the blood. At 50 per cent volume ratio (contrast medium to blood), the ionic contrast media converted the blood into a shear-thickening (increasing viscosity with increasing shear rate) suspension, indicating a marked rigidification of the single red cell, while the non-ionic contrast medium still produced shear-thinning, indicating less rigidification of the red cell (p<0.01). (Auth.)
[en] The protein fractions of blood serum containing HB antigen underwent disintegration to varying degrees, depending on the radiation ose. Dry preparations were more radiation-resistant. Gamma radiation reduced the immunochemical activity of HB-antigen. (author)
[en] Five ml of blood samples were taken from cervix, lung and other cancer patients treated with therapeutic radiation of 1,000r, 1,200r, 1,400r, 1,600r, 2,000r, 2,800r, 3,000r, 3,800r, 4,000r, 4,600r, 5,000r, 5,400r, 5,800r and 6,000r respectively. Chromosome preparations were obtained from invitro leukocyte culture and air drying method. Types of chromosome aberration rates were analyzed according to the radiation dose. Two types of abnormal chromosomes, dicentric and acentric chromosomes, were observed in the leukocytes. The polyploid cells were also found in the experimental groups. (author)
[en] Highly sensitive procedures for the determination of chromium in bio-environmental samples using neutron activation analysis, atomic absorption and gas chromatography have been developed in this laboratory. In the neutron activation procedure, dried samples are irradiated for up to 100 hours at a neutron flux in excess of 1014 neutrons cm-2s-1 for determinations at the parts per 109 level. Following irradiation, chromium is separated by distillation as chromylchloride and precipitated and counted as BaCrO4. Samples to be analysed by atomic absorption were digested using concentrated nitric acid followed by hydrogen peroxide, and injected into a Perkin Elmer model 2100 graphite furnace. For the gas chromatography determinations an EC detector in pulse mode with linearizer was used to detect chromium trifluoroacetylacetonate following separation on 3% OV-17. Analysis of a round-robin, freeze-dried urine sample by all three methods gave excellent agreement. (author)
[en] Complete text of publication follows. By considering the surface properties as a key factor in blood compatibility and biostability of a synthetic vessel, in the present study, the surface modification of a hybrid nanocomposite polyhedral oligomeric silsesquioxane (POSS) in to poly (carbonate-urea) urethane (CPU) , POSS-PCU, was done. Computer based modelling through response surface methodology (RSM) and central composite design (CCD) was used to optimise the processing conditions relating to plasma power output (30, 60 and 90 W), and (b) the duration of plasma exposure time (30, 75 and 120 sec) on the treatment process. It was found that optimal WCA (θ) for endothelial cells (EC) adhesion and retention which was reported 55 deg from supporting literature (equivalent to (γs = 51 mN/m), was easily achievable using the following experimental conditions: (I) power output at 30 W for 75 sec, (II) 90 W for 40 sec, and (III) 90 W for 55 sec in O2. The effect of plasma treatment on the film of POSS-PCU was studied through water contact angle (WCA), surface energy, ATR-FTIR, SEM and AFM. A comparison between platelet adhesion, hemolysis ratio and EC adhesion and MTT assays for cell assessment to both plasma treated and untreated samples were done and evaluated. This study showed that by developing a model, it is possible to investigate key experimental parameters to achieve reproducible and optimal wettability and surface energy (γs) values and hence modify the interfacial properties of biomaterials used in the design of vascular bypass grafts to enhance the endothelial cells (EC) response to biomaterials.
[en] Membranous nephropathy (MN), characterized by the presence of diffuse thickening of the glomerular basement membrane and subepithelial in situ immune complex disposition, is the most common cause of idiopathic nephrotic syndrome in adults, with an incidence of 5-10 per million per year. A number of studies have confirmed the relevance of several experimental insights to the pathogenesis of human MN, but the specific biomarkers of MN have not been fully elucidated. As a result, our knowledge of the alterations in histone methylation in MN is unclear. We used chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) to analyze the variations in a methylated histone (H3K9me3) in peripheral blood mononuclear cells from 10 MN patients and 10 healthy subjects. There were 108 genes with significantly different expression in the MN patients compared with the normal controls. In MN patients, significantly increased activity was seen in 75 H3K9me3 genes, and decreased activity was seen in 33, compared with healthy subjects. Five positive genes, DiGeorge syndrome critical region gene 6 (DGCR6), sorting nexin 16 (SNX16), contactin 4 (CNTN4), baculoviral IAP repeat containing 3 (BIRC3), and baculoviral IAP repeat containing 2 (BIRC2), were selected and quantified. There were alterations of H3K9me3 in MN patients. These may be candidates to help explain pathogenesis in MN patients. Such novel findings show that H3K9me3 may be a potential biomarker or promising target for epigenetic-based MN therapies
[en] To develop blood filters for reducing of transfusion, radiation technology used for the development of high quality and selectivity blood filters and to develop a one-pot multistep filter-based system for the complete separation of whole blood. As a result, the mesh, spherical, and mesh/spherical mixed 11 kinds of filters developed by using radiation grafting techniques have completed a blood compatibility and cell viability and non-enzymatic degradation assessment test. The performance of these developed filters compared to commercial filters, red blood cell recovery rate of 99% or more, platelet recovery rate of 100% or more, and a 99% or more leukocyte removal rate have achieved. In particular, this project successfully preempt and patented technologies to design a one-pot multistep blood filter system using the radiation technology. This research is expected to contribute significantly to public health and welfare, as well as import substitution and localization.
[en] The irradiation of blood products is indispensable in order to avoid the otherwise associated Graft-Versus-Host-Disease. Normally a dose of 30 Gy is considered to be adequate with a minimum acceptable value of 25 Gy. Approximately 67 blood irradiators are now in operation in Germany. By means of thermoluminescence-dosimeters both dose and dose distribution in the beaker were determined in 15 institutes. Three institutes were found that they did not irradiate the blood bags with the recommended dose of 30 Gy but with 35 Gy or even 40 Gy instead. The extreme percentage dose deviations of the investigated institutes were 83.2% and 156.8% of the nominal value. The minimum percentage dose was as low as 32.1% and as high as 96.6%. On the axis of rotation of the beaker, deviations of up to 57% occurred; an average deviation of 27% being typical. Ten institutes fell short of the minimum accepted dose of 25 Gy, whereas only 8 institutes achieved a mean dose in the beaker of 30 Gy or more with a maximum mean value of 38.8 Gy. Therefore, regulations regarding quality assurance and dosimetry procedures for blood irradiators appear indispensable. (orig.)
[de]Zur Vermeidung einer transfusionsassoziierten Graft-Versus-Host-Reaktion ist die Bestrahlung von Blutprodukten unerlaesslich. In der Regel wird eine Dosis von 30 Gy fuer notwendig erachtet, 25 Gy sollen nicht unterschritten werden. In Deutschland werden derzeit ca. 67 Geraete zur Bestrahlung von Blutkomponenten betrieben. Mittels Thermolumineszenz-Dosimetern wurden bei 15 Anlagen die Dosis und die Dosisverteilung im Bestrahlungsgefaess ermittelt. Die Nominaldosis der einzelnen Institute lag nicht einheitlich bei 30 Gy, drei Institute bestrahlten ihre Konserven mit Dosen zwischen 35 Gy und 40 Gy. In den Bestrahlungsbechern wurde maximal zwischen 83,2% und 156,8% der Nominaldosis erreicht. Minimal wurden 32,1% bis 96,6% der Nominaldosis gemessen. Nur 8 Institute erreichten eine mittlere Dosis von mindestens 30 Gy im Bestrahlungsgefaess, der hoechste Mittelwert betrug 38,8 Gy. An Punkten auf der Drehachse des Behaelters kamen Dosisabweichungen von bis zu 57% vor. Im Mittel waren 27% Dosis-Inhomogenitaet auf der Drehachse typisch. Die Minimaldosis von 25 Gy wurde bei 10 Instituten in Teilbereichen des Bechers unterschritten. Eine einheitliche Regelung zur Qualitaetssicherung und Dosimetrie an Blutbestrahlungsanlagen erscheint notwendig. (orig.)