[en] By the availability of various mutant resources in Arabidopsis, it is now possible to investigate mutant lines for almost every gene. Arabidopsis is then, not only a model plant for plant research, but also a model species in which it is possible to carry out 'saturation mutagenesis' for all genes, and to totally analyze each gene and mutant of one organism. One of the future goals of the 'phenome' project is to collect information about the knockout-type mutant phenotypes for each Arabidopsis gene. We have generated thousands of Dissociation (Ds) transposon-tagged lines, which have a single insertion because of an advantage of the Activator/Dissociation (Ac/Ds) system, and deposited it to the RIKEN BioResource Center. In this resource, we selected 4,000 transposon-tagged lines with a transposon insertion in gene-coding regions, and systematically observed the visible phenotype of each line as a first step of phenome analysis. In total, about 200 clear visible phenotypes were classified into 43 categories of morphological phenotypes. Phenotypic images have been entered into a searchable database. Parallel to this, we have been selecting homozygous transposon-insertional plants, which would be useful resources to detect other phenotypes besides the visible ones. We are setting three categories of measurement to search various traits for total phenome analysis, such as physical, chemical or biological methods. Recently, we started to investigate biologically-measured phenotypes, which are stress-responsive or conditional phenotypes, using homozygous mutant resources. We are also collecting any mutant phenotype information from published reports in journal research activity to make a comprehensive phenotype database of Arabidopsis genes and mutants. (author)

[en] Gluten-free (GF) dry egg pappardelle was prepared with tigernut flour (50%), chickpea flour (50%) and pregelatinized TNF (0, 5, 10%), and compared to plain pasta (100% durum wheat semolina). The GF pasta may have a significant higher content of insoluble fibre, minerals and fat rich in oleic acid and a similar protein content. It was not found any clear relationship between the flour functional properties and its proximate composition. The use of PG did not imply an improvement on the firmness but did provide some continuity to the pasta structure. (Author)

[en] Basal leaves frequently senesce before anthesis in high population density crops. This paper evaluates the hypothesis that quantitative and qualitative changes in the light environment associated with a high leaf area index (LAI) trigger leaf senescence in sunflower (Helianthus annuus L.) canopies. Mean leaf duration (LD, time from achievement of maximum leaf area) of leaf 8 was significantly (P < 0.05) reduced from 51 to 19 days as crop population density was increased from 0.47 to 4.76 plants m−2. High compared to low plant population density was associated with earlier reduction in the photosynthetically active radiation (PAR) and red/far-red ratio (R/FR) reaching the target leaf. However the changes in R/FR preceded those in PAR. When the light environment of individual leaves of isolated plants growing under field conditions was manipulated using filters and FR-reflecting mirrors, LD was positively and linearly related with the mean daily PAR (MDR) received in the FR- (no FR enrichment) treatments. FR enrichment of light reaching the abaxial surface of the leaf significantly (P < 0.05) reduced LD by 9 days at intermediate PAR levels with respect to FR-controls, but did not affect LD at the maximum PAR used in these experiments. However, when light reaching both leaf surfaces was enriched with FR, LD (for leaves receiving maximum PAR) was 13 days shorter than that of the FR- control. These results show that basal leaf senescence in sunflower is enhanced both by a decrease in PAR and by a decrease in R/FR. (author)

[en] The research work was conducted at NIAB, Faisalabad and consists of three combinations of both F4 and F5 populations along with their parents and check varieties. In F4 generation significant differences for fibre strength and lint weight were observed whereas in F5 generation significant difference in micronaire, maturity, fibre length, fibre strength, lint weight were observed. Cross combination NIAB-2008 x MNH-886 form F4 generation and from F5, cross combination NIAB-852 x MNH- 886 followed by NIAB-777 x MNH-886 showed high mean values for fibre maturity, lint weight, micronaire and seed cotton yield per plant. In F4 and F/sub 5/ generations, seed cotton yield per plant was significantly and positively correlated with ginning out turn (GOT %), fibre maturity, fibre length, micronaire and lint weight at genotypic level. In both F4 and F5, path coefficient analysis showed that ginning out turn, fibre uniformity, fibre strength and lint weight had direct positive effects on seed cotton yield at both phenotypic and genotypic level. From the present findings it is concluded that cotton breeders can enhance the efficiency of selection with the help of information generated on the relationship between components associated with fibre quality and yield itself in the early generations. Both F/sub 4/ and F/sub 5/ generations exhibited highest direct effects combined with positive correlation in ginning out turn%, fibre length and lint weight with seed cotton yield. The result showed that while making selection these fibre traits should be given more emphasis. (author)

[en] The results of a research carried out in order to study the reflectance, absorptance and transmittance spectra of sunflower leaves as related to their stem position are reported. Two sunflower hybrids, which differed for growth habitus (''Monica'', with low size, and ''Hysun-32'' with normal size) were used. Reflectance, absorptance and transmittance spectra were measured in vivo when the plants were in the phases of appearance of inflorescence and anthesis. The measures were taken with a portable spectroradiometer with an external integrating sphere. The radiation over the wavelength range from 400 to 1,100 mm was measured at intervals of 1 mm. Absorptance was calculated. The results have evidenced that leaf position, related to its metabolic age, influence the capacity of reflecting, absorbing and transmitting the radiation in the photosynthetically active region. In the PAR region the variations are different with the spectral band considered. The leaves in medium-high position, as compared to the top and bottom leaves, have lower reflectance and transmittance values and higher absorptance values. No difference emerged in the near infrared region

[en] In order to quantify the biological nitrogen fixation (BNF) and to evaluate the mycorrhiza effect in the BNF, an experiment was carried on by applying ¹5^N-ammonium sulphate and mycorrhiza fungi to the soil. The treatments included legumes: mucuna negra(Stizolobium atterrinum Piper et Tracv) and caupi (Vigna unguiculoata L. Walp). Two control plants: non nodulating soybean (Glycine max L.Merril) and rice (Oryza sativa), were used for measuring the fixed N in the legumes by isotope dilution method. Both legumes and control plants assimmilated the same ammounts of nitrogen from the soil and fertilizer. The greater N content in the legumnes was determined as coming from the fixed nitrogen. Rice and non nodulating soybean showed to be good controls for measuring biological nitrogen fixation using isotopic dilution method. The values of fixed nitrogen for legumes calculated using rice as control plant were slightly greater than those with non nodulating soybean, nevertheless there were no significant statistical differences between the values. The mucuna fixed more N than caupi in both mycorrhiza treatments (76.7, 66.6 and 56. 7 per cent of N fixed, respectively). The mycorrhiza increased dry matter yield (13.84 per cent), accumulation of N in the plant(14.85 per cent N) and the biological N fixation (16.06 per cent N-fixed) in caupi

[en] Diploid cotton (Gossypium arboreum L.) being an Old World cultivated cotton species, evolved in Indo-Pak subcontinent, has been known for conferring resistance to biotic and abiotic stresses. To the extent of our knowledge, there is no comprehensive report available on the characterization of G. arboreum germplasm. Hence, the present study was conducted to characterize 26 G. arboreum genotypes by deploying univariate and multivariate analysis in 2010 at NIBGE, Faisalabad. All these genotypes were characterized for boll weight, GOT percentage, micronaire value, staple length, fiber bundle strength and uniformity index. Genotypic variation was significant (p<0.01) for all the analyzed traits except boll weight. Maximum boll weight (2.47g) was observed for genotype 23718. GOT ranged from 18.75% (Haroonabad) to 36.94 percentage (DC-116).The finest fiber was obtained from synthetic (4.37 micro g/inch) and this genotype also exhibited the higher values for staple length (23.81 mm) and fiber bundle strength (27.37 g/tex). Range for uniformity index was observed from 76.19 percentage (Garohill) to 77.98 percentage (212). Principal component analysis (PCA) exhibited that first five components accounted for >63 percentage of the total variability. Cluster analysis identified four groups based on their agronomic properties. Significant relationships among different traits can be useful to select best genotypes having good fiber quality traits. These genotypes may prove a valuable resource to fuel the breeding efforts for not only broadening the genetic base of the newly developed material but can also add synergy to various cotton genomic projects. (author)

[en] Thirty-eight sunflower hybrids were evaluated for eight agronomic characters under field conditions at the National Agricultural Research Centre (NARC), Islamabad, during the Spring 2012. Significance for various characters alongwith better mean performance for yield indicated the diverse nature of hybrids and selection of better hybrids. In general, genotypic correlations were higher than the corresponding phenotypic correlations that revealed the genetic contribution for the associated response. Days to flower initiation, completion and maturity had positive and highly significant genotypic and phenotypic correlations with plant height. Head diameter had highly significant and genotypic positive association with the plant height. Similarly, oil contents were also significantly and positively associated with the plant height. Hundred seed weight had positive but non-significant association with the head diameter and the seed yield at both levels, which are an indicator of association among the associated traits that by improving head size and seed weight, the seed yield can be increased significantly. Seed yield had negative correlation with oil contents and suggested to break it either through conventional or novel breeding techniques to breed high yielding hybrids with maximum oil contents. The highest indirect positive effect was observed via days to flower initiation, followed by plant height, days to maturity, head diameter and oil contents, hence these characters may be given more emphasis while selecting high yielding sunflower hybrids. Cluster diagram based on Euclidean dissimilarity revealed five clusters at 25% linkage distance; cluster-I consisted of 9 genotypes, cluster-II 7, cluster-III 9 genotypes, cluster-IV 5 and cluster-V had 8 hybrids. Most of the locally developed hybrids fall under cluster-III and cluster V. The scattered diagram and cluster pattern were in coordination and the grouping was mainly attributed toward agronomic performance. (author)

[en] The article shows the importance of specially protected areas for preserving the gene pool of rare and useful plants in Siberia on the example of the ^Relic oaks^state landscape reserve. The landscape reserve is located on the territory of Eastern Siberia (Russia), on the two large floristic areas of Holarctic: Circumboreal and East-Asian. During the study, 696 species of higher vascular plants have been identified in the territory of the landscape reserve. The prevailing families are following: Asteraceae, Poaceae, Ranunculaceae, Rosaceae, Cyperaceae, Fabaceae, Caryophyllaceae, Brassicaceae, Apiaceae, and Scrophulariaceae. In the territory of landscape reserve, 4 floral complexes were identified: alpine, forest, steppe and meadow-alluvial, as well as 10 chorological groups. The feature of the flora was prevalence of forest and steppe plant species with the East Asian type of habitat. By the number of species, herbaceous plants in the landscape reserve dominate over arboreal plants, and shrubs have the largest share among arboreal plants. The spectrum of life forms of plants shows the climatic conditions of the territory at the border of large floral areas and zones of vegetation. On the studied territory, there are 171 species of higher vascular plants (24.5% of the total flora composition in the landscape reserve), are rare and protected in various regions of Siberia. (author)

[en] The information about the diversity of the crop for different attributes is important for their effective utilization in the breeding programs. The present investigation was carried out to characterize the 42 linseed (Linum usitatissimum L) genotypes by qualitative and quantitative agro-morphological traits and to describe the genetic variation. Eleven agro-morphological traits (germination days, initiation of flowers, days to maturity, height of plant, primary branches, number of capsules per plant, number of seeds per capsules per plant, seed yield of each plant, thousand seeds weight, seed size and seed color) were recorded to evaluate phenotypic variations among linseed genotypes. Three genotypes viz., 020885, 020890 and 020870 contributed noteworthy results on the basis of morphological traits. The diverse and significant trait was obtained from genotype 021200 (Pak) among total genotypes; having maximum number of primary branches per plant (10) as well as maximum number of seeds per capsule (10.3). Whereas, genotype 020885(Pak) contributed for the maximum weight of seeds per plant and for maximum weight of 1000 seeds among all the genotypes. The results also showed that agro-morphological attributes grouped genotypes belonged to different regions grouped together in same clusters. Out of 42 genotypes, 39 genotypes were clustered in 4 clusters and remaining 3 genotypes viz., 021160, 021200 and 035127 did not place in any cluster as they found to be most phenotypically diverse from all other genotypes. The information on the diversity and relationship with agro-morphological attributes will be helpful in the selection of populations in the breeding programs. (author)