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AbstractAbstract
[en] For myogenesis, new myotubes are formed by the fusion of differentiated myoblasts. In the sequence of events for myotube formation, intercellular communication through gap junctions composed of connexin 43 (Cx43) plays critical roles in regulating the alignment and fusion of myoblasts in advances of myotube formation in vitro. On the other hand, the relationship between the expression patterns of Cx43 and the process of myotube formation in satellite cells during muscle regeneration in vivo remains poorly understood. The present study investigated the relationship between Cx43 and satellite cells in muscle regeneration in vivo. The expression of Cx43 was detected in skeletal muscles on day 1 post-muscle injury, but not in control muscles. Interestingly, the expression of Cx43 was not localized on the inside of the basement membrane of myofibers in the regenerating muscles. Moreover, although the clusters of differentiated satellite cells, which represent a more advanced stage of myotube formation, were observed on the inside of the basement membrane of myofibers in regenerating muscles, the expression of Cx43 was not localized in the clusters of these satellite cells. Therefore, in the present study, it was suggested that Cx43 may not directly contribute to muscle regeneration via satellite cells
Primary Subject
Source
Available from http://dx.doi.org/10.1267/ahc.14056; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4427565; PMCID: PMC4427565; PMID: 26019374; PUBLISHER-ID: JST.JSTAGE/ahc/14056; PUBLISHER-MANUSCRIPT: 14056; OAI: oai:pubmedcentral.nih.gov:4427565; Copyright (c) 2015 The Japan Society of Histochemistry and Cytochemistry; This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Acta Histochemica et Cytochemica; ISSN 0044-5991;
; v. 48(2); p. 53-60

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Teixeira, John Douglas; Andrade Rosa, Ivone de; Brito, José; Maia de Souza, Yuli Rodrigues; Paulo de Abreu Manso, Pedro; Machado, Marcelo Pelajo; Costa, Manoel Luis; Mermelstein, Claudia, E-mail: mermelstein@ufrj.br2018
AbstractAbstract
[en] Highlights: • Gli-1 is present in aggregates near the nuclei of chick myoblasts and myotubes. • Gli-1 aggregates colocalized with gamma-tubulin positive-centrosomes. • Recombinant Shh added to muscle cells induced the nuclear translocation of Gli-1. • Gli-1 aggregates are storage sites for its rapid cellular redistribution. The Sonic Hedgehog signaling (Shh) pathway has been implicated in both proliferation of myoblast cells and terminal differentiation of muscle fibers, and contradictory results of these effects have been described. To clarify the role of Shh during myogenesis, we decided to study the effects of recombinant Shh and the distribution of Gli-1 during in vitro and in situ embryonic chick skeletal muscle differentiation at later stages of development. Gli-1 was found in small aggregates near the nucleus in mononucleated myoblasts and in multinucleated myotubes both in vitro and in situ chick muscle cells. Some Gli-1 aggregates colocalized with gamma-tubulin positive-centrosomes. Gli-1 was also found in striations and at the subsarcolemmal membrane in muscle fibers in situ. Recombinant Shh added to in vitro grown muscle cells induced the nuclear translocation of Gli-1, as well as an increase in the number of myoblasts and in the number of nuclei within myotubes. We suggest that Gli-1 aggregates observed in chick muscle cells near the nuclei of myoblasts and myotubes could be a storage site for the rapid cellular redistribution of Gli-1 upon specific signals during muscle differentiation.
Primary Subject
Source
S0006291X18324860; Available from http://dx.doi.org/10.1016/j.bbrc.2018.11.071; Copyright (c) 2018 The Authors. Published by Elsevier Inc.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 507(1-4); p. 496-502

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Radola, B.J.
Commission of the European Communities, Brussels (Belgium); Bundesforschungsanstalt fuer Lebensmittelfrischhaltung, Karlsruhe (F.R. Germany). Inst. fuer Strahlentechnologie1971
Commission of the European Communities, Brussels (Belgium); Bundesforschungsanstalt fuer Lebensmittelfrischhaltung, Karlsruhe (F.R. Germany). Inst. fuer Strahlentechnologie1971
AbstractAbstract
No abstract available
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Source
Apr 1971; 24 p; 10 figs.
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Report
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An siRNA-based screen in C2C12 myoblasts identifies novel genes involved in myogenic differentiation
Alwan, Rayan; Bruel, Ange-Line; Da Silva, Anne; Blanquet, Véronique; Bouhouche, Khaled, E-mail: veronique.blanquet@unilim.fr2017
AbstractAbstract
[en] Myogenesis is a highly regulated multi-step process involving myoblast proliferation and differentiation. Although studies over the last decades have identified several factors governing these distinct major phases, many of them are not yet known. In order to identify novel genes, we took advantage of the C2C12 myoblastic line to establish a functional siRNA screen combined with quantitative-imaging analysis of a large amount of differentiated myoblasts. We knocked down 100 preselected mouse genes without a previously characterized role in muscle. Using image analysis, we tracked gene-silencing phenotypes by quantitative assessment of cellular density, myotube quantity, myotube morphology and fusion index. Our results have revealed six genes involved in both stages of C2C12 myogenesis and 13 genes specific to the differentiation stage. These findings prove that our RNAi-based screen could be an efficient tool to detect clear and subtle phenotypes allowing the identification of new myogenic regulators in mammals. - Highlights: • A new optimized protocol to perform a functional siRNA based screen. • 100 preselected genes knocked-down in C2C12 myoblastic cell line. • 19 candidates genes identified as novel myogenic actors.
Primary Subject
Source
S0014-4827(17)30411-1; Available from http://dx.doi.org/10.1016/j.yexcr.2017.07.037; Copyright (c) 2017 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
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Fuller, Mark E.; Skowron, Mal; Tranter, Robert S.; Goldsmith, C. Franklin
Argonne National Laboratory (ANL), Argonne, IL (United States). Funding organisation: National Science Foundation (NSF) (United States); USDOE Office of Science - SC, Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division (United States); Brown University, Providence, RI (United States)2019
Argonne National Laboratory (ANL), Argonne, IL (United States). Funding organisation: National Science Foundation (NSF) (United States); USDOE Office of Science - SC, Basic Energy Sciences (BES) (SC-22). Chemical Sciences, Geosciences & Biosciences Division (United States); Brown University, Providence, RI (United States)2019
AbstractAbstract
[en] A new shock tube has been constructed for investigations of high-temperature chemical kinetics with an emphasis on combustion chemistry. This instrument includes a diaphragmless driver and electrical control of valving. A diaphragmless design significantly improves repeatability of experimental conditions vs the use of diaphragms and leads to an approximate order of magnitude reduction in turnaround time between experiments. Electrical control of valves, combined with diaphragmless operation, also enables remote and automated operation of the shock tube. The design allows for both incident and reflected shock experiments with multiple diagnostics. The performance of the shock tube is demonstrated by reproducing previous literature measurements on the unimolecular decomposition of isobutyl nitrite and cyclohexene.
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Secondary Subject
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OSTIID--1559884; AC02-06CH11357; Available from https://www.osti.gov/servlets/purl/1559884; DOE Accepted Manuscript full text, or the publishers Best Available Version will be available free of charge after the embargo period; arXiv:1905.09232
Record Type
Journal Article
Journal
Review of Scientific Instruments; ISSN 0034-6748;
; v. 90(6); vp

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External URLExternal URL
AbstractAbstract
No abstract available
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Journal Article
Journal
Journal of Food Science; v. 36(4); p. 549-551
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AbstractAbstract
[en] Muscular dystrophy and peripheral neuropathy have been linked to mutations in genes encoding nuclear envelope proteins; however, the molecular mechanisms underlying these disorders remain unresolved. Nuclear envelope protein p19A is a protein of unknown function encoded by a gene at chromosome 4q35. p19A levels are significantly reduced in human muscle as cells differentiate from myoblasts to myotubes; however, its levels are not similarly reduced in all differentiation systems tested. Because 4q35 has been linked to facioscapulohumeral muscular dystrophy (FSHD) and some adjacent genes are reportedly misregulated in the disorder, levels of p19A were analyzed in muscle samples from patients with FSHD. Although p19A was increased in most cases, an absolute correlation was not observed. Nonetheless, p19A downregulation in normal muscle differentiation suggests that in the cases where its gene is inappropriately re-activated it could affect muscle differentiation and contribute to disease pathology.
Primary Subject
Source
S0006-291X(09)01728-8; Available from http://dx.doi.org/10.1016/j.bbrc.2009.08.133; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 389(2); p. 279-283

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Tikka, Ajay C; Al-Sarawi, Said F; Abbott, Derek, E-mail: ajay.tikka@adelaide.edu.au2009
AbstractAbstract
[en] We present a normally closed, remotely actuated, secure coded, electrostatically driven active microvalve using passive components. This is carried out by utilizing the complex signal processing capabilities of two identical 5 × 2-bit Barker sequence encoded acoustic wave correlators. An electrostatically driven microchannel, comprising two conducting diaphragms as the top and bottom walls, is placed in between the compressor interdigital transducers of the two correlators. Secure interrogability of the microvalve is demonstrated by finite element modelling of the complete structure and the quantitative deduction of the code dependent microchannel actuation. Furthermore, the influence of the excited acoustic modes of the correlator on the microchannel deflection is investigated to optimize the microvalve design
Primary Subject
Source
S0964-1726(09)88442-5; Available from http://dx.doi.org/10.1088/0964-1726/18/4/045014; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
Journal
Smart Materials and Structures (Print); ISSN 0964-1726;
; v. 18(4); [8 p.]

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Ajzatskij, N.I.; Biller, E.Z.
15. Conference on the charged particle accelerators. Synopsis collection1996
15. Conference on the charged particle accelerators. Synopsis collection1996
AbstractAbstract
No abstract available
Original Title
Izgotovlenie i nastrojka uskoryayushchikh sektsij na baze neodnorodnykh diafragmirovannykh volnovodov
Primary Subject
Source
Aseev, A.A. (ed.); Akademiya Nauk Rossijskoj Federatsii, Moskva (Russian Federation); Gosudarstvennyj Komitet Rossijskoj Federatsii po Nauke i Tekhnologiyam, Moskva (Russian Federation); Ministerstvo Rossijskoj Federatsii po Atomnoj Ehnergii, Moskva (Russian Federation); Obedinennyj Institut Yadernykh Issledovanij, Dubna (Russian Federation); 92 p; ISBN 5-88738-012-8;
; 1996; p. 47; GNTs RF IFVEh; Protvino (Russian Federation); 15. Conference on the charged particle accelerators; 15. soveshchanie po uskoritelyam zaryazhennykh chastits; Protvino (Russian Federation); 22-24 Oct 1996

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Book
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Conference
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AbstractAbstract
[en] In this study, a new piezoactuator-based prototype microactuator is proposed with a hydraulic displacement amplification system. A piezoactuator is used to deflect a diaphragm which displaces a certain volume of hydraulic fluid into a smaller-diameter piston chamber, thereby amplifying the displacement at the other end of the piston. An electro-mechanical model is implemented to estimate the displacement of a multilayer piezoelectric actuator for the applied input voltage considering the hysteresis behavior. The displacement characteristics of the proposed microactuator are studied for triangular actuation voltage signal. Results of the experiments and simulation of the displacement behavior of the stacked piezoactuator and the amplified displacement of the prototype actuator were compared. Experimental results suggest that the mathematical model developed for the new piezoactuator-based prototype actuator is capable of estimating its displacement behavior accurately, within an error of 1.2%.
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Source
22 refs, 8 figs, 2 tabs
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Journal Article
Journal
Journal of Mechanical Science and Technology (Online); ISSN 1976-3824;
; v. 29(11); p. 4817-4822

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