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AbstractAbstract
[en] Azospirillum brasilense is a diazotroph that associates with important agricultural crops and thus has potential to be a nitrogen biofertilizer. The A. brasilense transcription regulator NifA, which seems to be constitutively expressed, activates the transcription of nitrogen fixation genes. It has been suggested that the nitrogen status-signaling protein GlnB regulates NifA activity by direct interaction with the NifA N-terminal GAF domain, preventing the inhibitory effect of this domain under conditions of nitrogen fixation. In the present study, we show that an N-terminal truncated form of NifA no longer required GlnB for activity and lost regulation by ammonium. On the other hand, in trans co-expression of the N-terminal GAF domain inhibited the N-truncated protein in response to fixed nitrogen levels. We also used pull-down assays to show in vitro interaction between the purified N-terminal GAF domain of NifA and the GlnB protein. The results showed that A. brasilense GlnB interacts directly with the NifA N-terminal domain and this interaction is dependent on the presence of ATP and 2-oxoglutarate
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Source
Available from http://dx.doi.org/10.1590/S0100-879X2012007500146; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3854208; PMCID: PMC3854208; PMID: 22983183; PUBLISHER-ID: S0100-879X2012007500146; OAI: oai:pubmedcentral.nih.gov:3854208; This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Brazilian Journal of Medical and Biological Research; ISSN 0100-879X;
; v. 45(12); p. 1135-1140

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External URLExternal URL
Page, Don N., E-mail: profdonpage@gmail.com2014
AbstractAbstract
[en] The AMPS argument for black hole firewalls seems to arise not only from the assumption of local effective field theory outside the stretched horizon but also from an overcounting of internal black hole states that include states that are singular in the past. Here I propose to exclude such singular states by Extreme Cosmic Censorship (the conjectured principle that the universe is entirely nonsingular, except for transient singularities inside black and/or white holes). I argue that the remaining set of nonsingular realistic states do not have firewalls but yet preserve information in Hawking radiation from black holes that form from nonsingular initial states
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Source
Available from http://dx.doi.org/10.1088/1475-7516/2014/06/051; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
Journal
Journal of Cosmology and Astroparticle Physics; ISSN 1475-7516;
; v. 2014(06); p. 051

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Xia Yu; Jiang Baichun; Zou Yongxin; Gao Guimin; Shang Linshan; Chen Bingxi; Liu Qiji; Gong Yaoqin, E-mail: yxg8@sdu.edu.cn2008
AbstractAbstract
[en] Imitation Switch (ISWI) is a member of the SWI2/SNF2 superfamily of ATP-dependent chromatin remodelers, which are involved in multiple nuclear functions, including transcriptional regulation, replication, and chromatin assembly. Mammalian genomes encode two ISWI orthologs, SNF2H and SNF2L. In order to clarify the molecular mechanisms governing the expression of human SNF2L gene, we functionally examined the transcriptional regulation of human SNF2L promoter. Reporter gene assays demonstrated that the minimal SNF2L promoter was located between positions -152 to -86 relative to the transcription start site. In this region we have identified a cAMP-response element (CRE) located at -99 to -92 and a Sp1-binding site at -145 to -135 that play a critical role in regulating basal activity of human SNF2L gene, which were proven by deletion and mutation of specific binding sites, EMSA, and down-regulating Sp1 and CREB via RNAi. This study provides the first insight into the mechanisms that control basal expression of human SNF2L gene
Primary Subject
Source
S0006-291X(08)00183-6; Available from http://dx.doi.org/10.1016/j.bbrc.2008.01.111; Copyright (c) 2008 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 368(2); p. 438-444

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Podgorsak, E.; Moran, P.R.
Wisconsin Univ., Madison (USA). Dept. of Physics
Wisconsin Univ., Madison (USA). Dept. of Physics
AbstractAbstract
No abstract available
Source
nd; 9 p
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Report
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Hung, L.-W.; Wang, I.X.; Nikaido, K.; Liu, P.-Q.; Ames, G.F.-L.; Kim, S.-H.
Ernest Orlando Lawrence Berkeley National Lab., Advanced Light Source, Berkeley, CA (United States). Funding organisation: US Department of Energy (United States)1998
Ernest Orlando Lawrence Berkeley National Lab., Advanced Light Source, Berkeley, CA (United States). Funding organisation: US Department of Energy (United States)1998
AbstractAbstract
No abstract available
Source
LBNL/ALS--1537; AC03-76SF00098; Journal Publication Date: December 17 1998
Record Type
Journal Article
Journal
Nature (London); ISSN 0028-0836;
; v. 396(6712); [10 p.]

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Svoboda, P.; Harms-Ringdahl, M.
Dept. of Molecular Genome Research, Stockholm Univ., Stockholm (Sweden)
Radiobiology 2000: advances in fundamental and clinical radiobiology. Programme and abstracts: 1st international congress of the South African Radiobiology Society (SARS) in conjunction with the South African Association of Physicists in Medicine and Biology (SAAPMB) and the University of Stellenbosch, 10-13 December 2000, Music Conservatoire, University of Stellenbosch2000
Dept. of Molecular Genome Research, Stockholm Univ., Stockholm (Sweden)
Radiobiology 2000: advances in fundamental and clinical radiobiology. Programme and abstracts: 1st international congress of the South African Radiobiology Society (SARS) in conjunction with the South African Association of Physicists in Medicine and Biology (SAAPMB) and the University of Stellenbosch, 10-13 December 2000, Music Conservatoire, University of Stellenbosch2000
AbstractAbstract
No abstract available
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Source
South African Radiobiology Society (South Africa); South African Association of Physicists in Medicine and Biology, Pretoria (South Africa); Univ. of Stellenbosch, Stellenbosch (South Africa); [98 p.]; Dec 2000; [1 p.]; Radiobiology 2000: 1. international congress of the South African Radiobiology Society; Stellenbosch (South Africa); 10-13 Dec 2000; Available from the Dept. of Radiation Oncology, Faculty of Medicine, Univ. of Stellenbosch, P.O. Box 19063, Tygerberg, 7505, South Africa; Published in summary form only
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Miscellaneous
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Conference
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AbstractAbstract
[en] Highlights: ► ATP-treated sciatic explants shows the decreased expression of p75NGFR. ► Extracellular ATP inhibits the expression of phospho-ERK1/2. ► Lysosomal exocytosis is involved in Schwann cell dedifferentiation. ► Extracellular ATP blocks Schwann cell proliferation in sciatic explants. -- Abstract: After nerve injury, Schwann cells proliferate and revert to a phenotype that supports nerve regeneration. This phenotype-changing process can be viewed as Schwann cell dedifferentiation. Here, we investigated the role of extracellular ATP in Schwann cell dedifferentiation and proliferation during Wallerian degeneration. Using several markers of Schwann cell dedifferentiation and proliferation in sciatic explants, we found that extracellular ATP inhibits Schwann cell dedifferentiation and proliferation during Wallerian degeneration. Furthermore, the blockage of lysosomal exocytosis in ATP-treated sciatic explants is sufficient to induce Schwann cell dedifferentiation. Together, these findings suggest that ATP-induced lysosomal exocytosis may be involved in Schwann cell dedifferentiation.
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Source
S0006-291X(12)02232-2; Available from http://dx.doi.org/10.1016/j.bbrc.2012.11.057; Copyright (c) 2012 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 430(2); p. 852-857

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Nishizaki, Tomoyuki, E-mail: tnishiaki@bioresorganization.com2018
AbstractAbstract
[en] Higghlights• Profilin associates with activated PKCε. • Profilin does not activate PKCε directly. • Profilin facilitates PKCε activation by accelerating ATP supply. Profilin catalyzes the exchange of actin-bound ADP to ATP. The present study investigated the role of profilin in PKCε activation. Profilin associated with PKCε in differentiated PC-12 cells under the basal conditions, which was inhibited by the PKC inhibitor GF109203X. The selective PKCε activator DCP-LA markedly increased the association, which was clearly prevented by GF109203X. The basal PKC activity in PC-12 cells was attenuated by knocking-down profilin, while the basal activities of PKA and CaMKII were not affected. DCP-LA enhanced the PKC activity to approximately 3.5 folds of the basal levels, and the effect was suppressed by knocking-down profilin. In the cell-free system, PKCε was not activated by profilin alone. DCP-LA activated PKCε in an ATP concentration (2–500 μM)-dependent manner, and addition of profilin shifted the ATP concentration/DCP-LA-induced PKCε activity relation curve to the left (the direction of lower ATP concentrations). Taken together, the results of the present study indicate that profilin binds to activated PKCε and facilitates PKCε activation by accelerating ATP supply to PKCε.
Primary Subject
Source
S0006291X18322769; Available from http://dx.doi.org/10.1016/j.bbrc.2018.10.114; Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 506(4); p. 918-922

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Sakamaki, Jun-ichi; Daitoku, Hiroaki; Yoshimochi, Kenji; Miwa, Masanao; Fukamizu, Akiyoshi, E-mail: akif@tara.tsukuba.ac.jp2009
AbstractAbstract
[en] Forkhead box O (FOXO) transcription factors play an important role in a wide range of biological processes, including cell cycle control, apoptosis, detoxification of reactive oxygen species, and gluconeogenesis through regulation of gene expression. In this study, we demonstrated that PARP-1 functions as a negative regulator of FOXO1. We showed that PARP-1 directly binds to and poly(ADP-ribosyl)ates FOXO1 protein. PARP-1 represses FOXO1-mediated expression of cell cycle inhibitor p27Kip1 gene. Notably, poly(ADP-ribosyl)ation activity was not required for the repressive effect of PARP-1 on FOXO1 function. Furthermore, knockdown of PARP-1 led to a decrease in cell proliferation in a manner dependent on FOXO1 function. Chromatin immunoprecipitation experiments confirmed that PARP-1 is recruited to the p27Kip1 gene promoter through a binding to FOXO1. These results suggest that PARP-1 acts as a corepressor for FOXO1, which could play an important role in proper cell proliferation by regulating p27Kip1 gene expression.
Primary Subject
Source
S0006-291X(09)00475-6; Available from http://dx.doi.org/10.1016/j.bbrc.2009.03.022; Copyright (c) 2009 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 382(3); p. 497-502

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AbstractAbstract
[en] A new ceramid (2S*, 3S*, 4E, 8E)-2N-(hexadecanoyl)-4 (E), 8 (E)-docosadiene-1, 3-diol (1), along with the previously identified one (2S*, 3S*, 4E, 8E)-2N-(tetradecanoyl)-4 (E), 8 (E)-icosadiene-1, 3-diol (2), and the dihydroxy steroid, 24-methyl-cholest-5 (E), 22 (E)-dien-3?,7?-diol (3) were identified from the Red Sea black coral Antipathies dichotoma. Extensive spectroscopic data 1D and 2D- NMR, UV, IR and MS were the bases for structure elucidation of all isolated metabolites. The spectral values for compound 3 are reported here for the first time. All compounds have been estimated toward their cytotoxicity, against several cancer cell lines; HepG2, WI 38, VERO and MCF-7. They showed strong to moderate cytotoxic activities. Compounds 1 and 2 displayed strong antiproliferative activities against HepG2 and MCF-7 cell lines. 3 displayed almost weak activity towards all investigated cell lines. (author)
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Record Type
Journal Article
Journal
Journal of the Chemical Society of Pakistan; ISSN 0253-5106;
; v. 38(3); p. 553-557

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