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[en] Ferritin was purified 33,000-fold from the plasma of patients with idiopathic hemochromatosis. The plasma ferritin was labeled with 131I and injected into 2 normal men. Clearance was found to be relatively slow, with 50% 131I-ferritin remaining in the plasma at 27-30 hr. The fraction of plasma ferritin that bound to concanavalin-A was found to be cleared more slowly than the nonbinding fraction. These findings confirm our previous suggestion that glycosylation is a major factor prolonging the survival of ferritin in the plasma, but differ from the results of earlier studies in experimental animals and preterm infants, which indicated very rapid plasma ferritin turnover
Journal
Blood; ISSN 0006-4971; 
; v. 60(4); p. 827-833
; v. 60(4); p. 827-833
No abstract available
Journal
Annales de Biologie Clinique (Paris); ISSN 0003-3898; ; v. 37(2); p. 118
; v. 37(2); p. 118
No abstract available
Journal
Annales d'Endocrinologie (Paris); ISSN 0003-4266; ; v. 43(2); p. 152
; v. 43(2); p. 152
[en] Using an ion exchange resin, a sensitive radioimmunoassay for plasma arginine vasopressin (AVP) was developed. This assay was characterized by the absence of blank values, an excellent recovery rate, great sensitivity (0.1 pg of AVP was significantly detected) and reproducibility. In 8 normal men, plasma AVP after overnight dehydration was 1.57+-0.17 pg/ml, and dropped to 0.58+-0.11 pg/ml after 20 ml/kg oral water loading. Significant correlations between plasma AVP levels and plasma or urinary osmolality confirm the validity of this assay. In complete pituitary diabetes insipidus (n=4) plasma AVP was undetectable whereas it was frankly increased in Schwartz-Bartter syndrome (3 to 33 pg/ml, n=8)
[fr]
Un dosage radioimmunologique de l'hormone antidiuretique (AVP) plasmatique est presente. Il est caracterise par un procede original d'extraction-purification sur resine echangeuse d'ions qui elimine les substances plasmatiques susceptibles de perturber la reaction hormone-anticorps, un excellent rendement, une grande sensibilite (0.1 pg d'AVP est detecte de facon significative) et une grande reproductibilite. Les taux plasmatiques chez 8 sujes normaux sont de l'ordre de 1.57+-0.17 pg/ml apres une nuit de restriction hydrique et de 0.58+-0.11 pg/ml apres une charge hydrique de 20 ml/kg. L'existence d'une relation significative entre l'AVP plasmatique et l'osmolalite plasmatique ou urinaire confirme la validite de ce dosage. Dans le diabete insipide hypophysaire (n=4) l'AVP est indetectable alors qu'elle est franchement elevee dans les syndromes de Schwartz-Bartter (3 a 33 pg/ml, n=8)Journal
Annales d'Endocrinologie (Paris); ISSN 0003-4266; ; v. 41(5); p. 379-385
; v. 41(5); p. 379-385
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