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[en] Radio-immunoassay of serum gastrin may now be carried out in all laboratories of radio-immunology. Comparison of two commercial kits A: Schwartz-Mann and B: CEA-SORIN according to criteria of specificity, sensitivity and reproducibility within and between systems, shows that they both permit the detection of pathological hypergastrinemias (Zollinger Ellison and atropic gastritis). Both kits have an identical intrasystem coefficient of variation (10 p. cent and 7 p. cent fort A, 5 p. cent and 8 p. cent for B) on the other hand, the inter-system coefficient of variation is better for kit B (22.4 p. cent and 37 p. cent for kit A, and 11.5 p. cent and 14.2 p. cent for kit B). The normal values for each kit are quite different: 97 . 64 pg.ml-1 for A and 51 . 23 pg.ml-1 for B preventing one from comparing estimations carried out with two different kits
[fr]
Le dosage radio-immunologique de la gastrine serique immunoreactive est actuellement accessible a tous les laboratoires de radio-immunologie. La comparaison de deux trousses commerciales A: Schwartz-Mann et B: CEA-Sorin selon les criteres de specificite, de sensibilite et de reproductibilite intra et intersysteme montre qu'elles permettent toutes deux le depistage des hypergastriemies pathologiques (Zollinger et Ellison et gastrite atrophique). Les deux trousses ont un coefficient de variation intrasysteme identique (10% et 7% pour A; 5% et 8% pour B), par contre le coefficient de variation intersysteme est meilleur pour la trousse B (22,4% et 37% pour A; 11,5% et 14,2% pour B). Les valeurs normales pour les deux trousses sont tres differentes: 97 . 64 pg.ml-1 pour A et 51 . 23 pg.ml-1 pour B empechant de comparer les dosages effectues avec deux trousses differentesJournal
Annales de Biologie Clinique (Paris); ISSN 0003-3898; 
; v. 38(2); p. 129-131
; v. 38(2); p. 129-131
[en] The aim of this work is to estimate the commercial method developed by the CEA for the radioimmunological determination of serum thyreostimuline. The study covers a year of clinical use. The method is described and the results given for more than 1000 determinations, performed on both reference and pathological subjects. This method is found to have met the needs of clinical investigation
[fr]
On se propose d'evaluer la methode commerciale mise au point par le CEA pour le dosage radioimmunologique de la thyreostimuline du serum. L'etude porte sur un an d'utilisation clinique. On decrit a la fois la methode et les resultats portant sur plus de 1000 determinations effectuees tant chez des sujets temoins qu'en pathologie. On constate que la methode utilisee a repondu aux besoins de l'investigation cliniqueSource
Kellershohn, C.; Raynaud, C. (eds.); CEA Centre d'Etudes Nucleaires de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Biologie; p. 170-173; ISBN 2727200013; ; 1975; 17. French language symposium on nuclear medicine; Paris, France; 02 Jun 1975
; 1975; 17. French language symposium on nuclear medicine; Paris, France; 02 Jun 1975
No abstract available
Journal
Annales d'Endocrinologie (Paris); ISSN 0003-4266; ; v. 40(5); p. 10A
; v. 40(5); p. 10A
[en] Triiodothyronine concentration was determined in human serum and in thyroid extracts by two radioimmunoassay technics. Both antibodies, radioactive T3, stable T3, protein-binding-hormone inhibitors, methods used to separate the free from the bound fractions were different. Each factor from a method was substituted to the homologue of the other method. The results occasionnally showed a variation of 32% in the first method and 50% in the second one. However the results gave a correct interpretation of the triiodothyronine values measured in human serum and in thyroid extracts
[fr]
La mesure de la concentration en triiodothyronine (T3) de serums humains et d'extraits thyroidiens a ete effectuee par deux methodes radio-immunologiques qui differaient par l'anticorps, la T3 radioactive, la T3 stable, l'inhibiteur de la liaison de l'hormone a la proteine et le moyen de separer les fractions libre et liee. Chaque parametre d'une methode a ete substitue au parametre homologue de l'autre methode. Les resultats montrent parfois une variation allant jusque 32% avec la premiere methode et 50% avec la deuxieme. Cependant ils permettent une interpretation correcte des valeurs de triiodothyronine mesurees dans le serum humain et dans les extraits thyroidiensJournal
Annales de Biologie Clinique (Paris); ISSN 0003-3898; ; v. 38(3); p. 169-173
; v. 38(3); p. 169-173
[en] A boronate affinity monolith with improved affinity and selectivity for glycoproteins was prepared starting from two monomers. The first is 3-aminopropyltriethoxysilane-methacrylic acid (APTES-MAA), and the other is a polyhedral oligomeric silsesquioxane (POSS) monomer. In the next step, 3-(acrylamido)benzeneboronic acid was adopted as boronate affinity ligand, and ethylene glycol dimethacrylate as the crosslinker, and iso-propanol and octanol as binary porogens. The synergistic effect of APTES-MAA and POSS warrants good affinity and selectivity for glycoproteins, which results in a number of attractive features including (a) a wide operation pH range (from 5 to 8); (b) higher enrichment factors ranging from 19.3 to 20.6; (c) greater recoveries of glycoproteins between 95.8 and 107.1%; (d) lower relative standard deviations of ≤4.2%. Compared to the corresponding APTES-MAA/POSS-free monolith, the new boronate material had 1.7-fold increased glycoprotein recovery from complex samples. Glycoproteins in 500-fold diluted serum samples can be enriched by the boronate monolith. .
Journal
No abstract available
Journal
Annales d'Endocrinologie (Paris); ISSN 0003-4266; ; v. 41(5); p. 435-436
; v. 41(5); p. 435-436
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