Results 1 - 10 of 12507
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[en] Monoclonal gammopathies (MGs) are hematological diseases characterized by high levels of a monoclonal immunoglobulin (Ig) or M-protein. Within this group are patients with more than one M-protein, referred to as double MGs (DMGs). The M-proteins in DMG patients may have different heavy chain (HC) isotypes that are associated with different light chains (LCs), or different HCs that are LC matched. In this study, we examined the clonal relatedness of the M-proteins in the latter type in a cohort of 14 DMG patients. By using PCR, we identified 7/14 DMG patients that expressed two Ig HC isotypes with identical Ig HC variable (IGHV), diversity (IGHD), joining (IGHJ), and complementarity determining region (HCDR3) sequences. Two additional DMG patients had two Ig transcripts using the same IGHV, IGHD and IGHJ genes but with slight differences in variable region or HCDR3 mutations. LC analysis confirmed that a single LC was expressed in 3/7 DMG patients with identical HC transcripts and in the two DMGs with highly similar transcripts. The PCR findings were confirmed by immunofluorescence for HC and LC expression. Clonally related HC-dissimilar/LC-matched DMGs may occur often and defines a new subtype of MG that may serve as a tool for studies of disease pathogenesis
[en] Two developments in the process of automated protein model building in the Buccaneer software are described: the use of a database of protein fragments in improving the model completeness and the assembly of disconnected chain fragments into complete molecules. Two developments in the process of automated protein model building in the Buccaneer software are presented. A general-purpose library for protein fragments of arbitrary size is described, with a highly optimized search method allowing the use of a larger database than in previous work. The problem of assembling an autobuilt model into complete chains is discussed. This involves the assembly of disconnected chain fragments into complete molecules and the use of the database of protein fragments in improving the model completeness. Assembly of fragments into molecules is a standard step in existing model-building software, but the methods have not received detailed discussion in the literature
[en] Highlights: • A novel colorimetric strategy for sensitive DNA detection based on AuNPs assemblies induced by PCR product. • The proposed strategy avoids the denaturation of PCR product to allow efficient hybridization with AuNPs-DNA probes. • The sensitivity of this method is significantly improved, due to PCR amplification and the assemblies of AuNPs. • The universality of the method is likely to be good, any target DNA can be analyzed with the same AuNPs-DNA probes. - Abstract: We developed a novel strategy for rapid colorimetric detection of specific DNA sequence based on gold nanoparticles assemblies induced by polymerase chain reaction (PCR) product. In the presence of target DNA, the two DNA-functionalized AuNP probes selectively hybridized with the prohibited nucleic acid segments of two primers owing to the zipping off of the hairpin structures during PCR process, resulted in the aggregation of AuNPs with a concomitant color change from red to blue-purple. It is a convenient and universal method for sensitive DNA detection with no need for any further post-treatment of the PCR products. Most importantly, our method showed a low limit of detection (LOD) of 4.3 fM with a wide range of target DNA from 16 fM to 1.6 nM. Owing to the versatility and low cost, the proposed strategy could be extremely useful for a wide range of applications, providing a promising tool for rapid disease diagnostics and gene sequencing.
[en] The feasibility of glycosylation post-purification has been demonstrated by introducing glucose into the model protein lysozyme via a novel reaction that is compatible with biological samples. The crystallization of glycoproteins is one of the challenges to be confronted by the crystallographic community in the frame of what is known as glycobiology. The state of the art for the crystallization of glycoproteins is not promising and removal of the carbohydrate chains is generally suggested since they are flexible and a source of heterogeneity. In this paper, the feasibility of introducing glucose into the model protein hen egg-white lysozyme via a post-purification glycosylation reaction that may turn any protein into a model glycoprotein whose carbohydrate fraction can be manipulated is demonstrated
[en] The double row transfer matrix of the open spin chain is diagonalized and the Bethe Ansatz equations are also derived by the algebraic Bethe Ansatz method including the so far missing case when the residual symmetry is . In this case the boundary breaks the “rank” of the symmetry leading to nonstandard Bethe Ansatz equations in which the number of Bethe roots is less than as it was in the periodic case. Therefore these cases are similar to soliton-nonpreserving reflections.
[en] We define and study the heat conductivity κ and the Lyapunov spectrum for a modified 'ding-a-ling' chain undergoing steady heat flow. Free and bound particles alternate along a chain. In the present work, we use a linear gravitational potential to bind all the even-numbered particles to their lattice sites. The chain is bounded by two stochastic heat reservoirs, one hot and one cold. The Fourier conductivity of the chain decreases smoothly to a finite large-system limit. Special treatment of satellite collisions with the stochastic boundaries is required to obtain Lyapunov spectra. The summed spectra are negative, and correspond to a relatively small contraction in phase space, with the formation of a multifractal strange attractor. The largest of the Lyapunov exponents for the ding-a-ling chain appears to converge to a limiting value with increasing chain length, so that the large-system Lyapunov spectrum has a finite limit. copyright 1998 The American Physical Society
[en] The results of studies into microporous scaffolds based on polycaprolactone, in particular, involving nanoparticles and microparticles of modified (silicon-containing) hydroxyapatite (hybrid scaffolds) are presented. When hydroxyapatite particles are used during the electrospinning of polymer scaffolds, their porosity is found to increase substantially and a structure with nanofibers and microfibers can be created. X-ray phase analysis demonstrates that the characteristic lines of polycaprolactone and hydroxyapatite exist in the 3D hybrid scaffold structure. According to the data of infrared (IR) spectroscopy of the hydroxyapatitepowder precursor, (SiO4)4– ions are embedded in its lattice. The results of studies into the surface wettability indicate that the contact angles of wetting with water are smaller for hybrid scaffolds than for pure polycaprolactone scaffolds. Adhesive and proliferative activity tests of human mesenchymal stem cells (MSCs) performed upon hybrid-scaffold cultivation on the surface, as well as histologic investigations, indicate the high biocompatibility of the samples. On the basis of a polymerase chain reaction, it is revealed that the differentiation of MSCs occurs in the osteogenic direction. On account of a porous structure, hybrid scaffolds can be employed to recover bone-tissue defects.
[en] Complete text of publication follows. Observations of timing sequences of substorms expected in various onset mechanisms are examined by using a space-time diagram, which correlates observed space signatures and auroral signatures on the ground during substorm onset. Results from a statistical study of eleven substorms show that signatures in the mid-tail (x ∼ 15-25 RE) typically occur before the ground signatures and those in the near tail (x ∼ 10 RE), and the signatures in both regions observed prior to the substorm onset often occur in an advanced time range which was shorter than those expected from propagation between different regions. It suggests that the disturbance onsets in different active regions do not seem to have a simple causal chain relationship between them as described by reconnection or current disruption models of substorms. The activation of perturbed fields and plasma flows in space including the signatures of reconnection and current disruption may occur in multiple localized regions throughout the stressed tail current sheet. The activation seems to be continuously observed well after the substorm onset. These results to some extent are consistent with suggested Global Alfvenic Interaction considerations, in which the substorm onset is the result of Alfvenic Interaction in the global current systems.
[en] Mass defect and binding energy, droplet model, energy balance, chain reaction, water as moderator and coolant, power control, afterheat. (orig.)
[de]Massendefekt und Bindungsenergie, Troepfchenmodell, Energiebilanz, Kettenreaktion, Wasser als Moderator und Kuehlmittel, Leistungsregelung, Nachwaerme. (orig.)