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[en] Mitochondrial dynamics, including constant fusion and fission, play critical roles in maintaining mitochondrial morphology and function. Here, we report that developmentally regulated GTP-binding protein 2 (DRG2) regulates mitochondrial morphology by modulating the expression of the mitochondrial fission gene dynamin-related protein 1 (Drp1). shRNA-mediated silencing of DRG2 induced mitochondrial swelling, whereas expression of an shRNA-resistant version of DRG2 decreased mitochondrial swelling in DRG2-depleted cells. Analysis of the expression levels of genes involved in mitochondrial fusion and fission revealed that DRG2 depletion significantly decreased the level of Drp1. Overexpression of Drp1 rescued the defect in mitochondrial morphology induced by DRG2 depletion. DRG2 depletion reduced the mitochondrial membrane potential, oxygen consumption rate (OCR), and amount of mitochondrial DNA (mtDNA), whereas it increased reactive oxygen species (ROS) production and apoptosis. Taken together, our data demonstrate that DRG2 acts as a regulator of mitochondrial fission by controlling the expression of Drp1. - Highlights: • DRG2 depletion increased mitochondrial swelling. • DRG2 depletion inhibited the expression of Drp1. • Overexpression of DRG2 or Drp1 rescued mitochondrial shape in DRG2 depleted cells. • DRG2 depletion induced mitochondrial dysfunction.
[en] Peat production in Finland in 2000 was 12.150 million m3 (10.406 TWh). The share of sod peat was 1 096500 m3 (9%), the share of milled peat 10 023 753 m3 (83%), and that of horticultural peat 1 029 191 m3 (8%) of the total. The total peat production area in 2000 was 43 022 ha. The consumption energy peat in 2000 was 15.535 million m3 (14.414 TWh), the share of sod peat being 883 000 m3 (1.135 TWh), and that of milled peat 14.652 million m3 (13.279 TWh). The prices of sod peat in 2000 varied between 32.4 and 33.2 FIM/MWh at the mire, and between 45.0 and 46.2 FIM/MWh at the mill. The price of milled peat varied between 43.7 and 44.6 FIM/MWh at the mill. About 134.948 tons of peat was exported from Finland in 2000, the value of the deliveries being 60.568 million FIM
[en] The main fuel component for commercial nuclear power reactors is Uranium. When compared to fossil fuels, it has a competitive edge due to factors such as economics and environmental conditions and in particular due to its international market availability. Uranium world demand reached to 67 320 tU in 2004, which was covered with additional sources. To project the uranium markets behavior requires to know and to accept some conditions tied to the demand, such as the electrical world consumption, the greenhouse effect; water desalination, production of hydrogen, industrial heat, the innovative development of nuclear reactors, and the average time of 10 years between the beginning of exploration programs and definition of deposits, which it owes mainly to the difficulty of achieving the legal, environmental and local community authorizations, to open new mining centers. Uranium market future projections, made by IAEA experts in 2001, that considered middle and high demand scenarios, concluded that cumulatively to year 2050, with regard to demand it will be required 5.4 and 7.6 million tons of uranium respectively, and with regard to the uranium price, it should present a sustained increase. In the last years the situation of the uranium market has changed dramatically. In August 2006 the price of uranium reached to USD 106/kgU in the spot market, surpassing all the made projections. The increase in price that has stayed in rise in the last five years is reactivating the prospection and exploration efforts anywhere in the world, and competition between prospective areas of potential resources mainly in less explored territories
[en] This study was carried out in Bulamogi, Uganda, with the main objective of determining preferred firewood species, their harvesting and consumption patterns. Data collected through household and key-informants interviews, using open- and close-ended questionnaires and semi-structured interviews, showed that 48 plant species in 36 genera and 20 families are used as firewood. These plants have other uses as herbal medicines and in traditional cultural rites. There is almost total dependence on firewood for domestic cooking and small-scale industries. Firewood is used to fire bricks (55%), distil spirits (26%), cure fish (10%), cook food in restaurants (6%) and to produce charcoal (3%). Firewood for domestic use is collected mainly by women, and largely comprises of dead wood. The distances travelled to firewood collection areas are short and little time is spent. The harvesting of firewood for domestic use may have a lower direct impact on the native flora, than the harvesting of fuelwood for commercial use by small-scale industries and to make charcoal, which requires large amounts of wood that is often green. According to the community response, firewood is abundant but declining. This decline may be related to increasing demands generated by the growing human population of Bulamogi, and growing national need for charcoal. Cultural taboos that have hitherto played an important role in plant conservation appear to be weakening. There is limited trading of firewood in the community. (author)
[en] The evolutionary adaptation was approached on the thermotolerant yeast Kluyveromyces marxianus NIRE-K3 at 45°C on xylose as a sole source of carbon for enhancement of xylose uptake. After 60 cycles, evolved strain K. marxianus NIRE-K3.1 showed comparatively 3.75- and 3.0-fold higher specific growth and xylose uptake rates, respectively, than that of native strain. Moreover, the short lag phase was also observed on adapted strain. During batch fermentation with xylose concentration of 30 g l−1, K. marxianus NIRE-K3.1 could utilize about 96% of xylose in 72 h and produced 4.67 and 15.7 g l−1 of ethanol and xylitol, respectively, which were 9.72- and 4.63-fold higher than that of native strain. Similarly, specific sugar consumption rate, xylitol, and ethanol yields were 5.07-, 1.15-, and 2.44-fold higher as compared to the native strain, respectively. The results obtained after evolutionary adaptation of K. marxianus NIRE-K3 show the significant improvement in the xylose utilization, ethanol and xylitol yields, and productivities. By understanding the results obtained, the significance of evolutionary adaptation has been rationalized, since the adapted culture could be more stable and could enhance the productivity.
[en] It is claimed that the consumption of natural gas for industrial uses in Denmark is increasing. 7% of industrial companies are responsible for 43% of total consumption and the direct use of natural gas in industrial processes gives 30-40% of total consumption. Detailed data are presented on the number of contracts held by Danish gas companies and the volume of gas delivered, on the number of contacts and volumes delivered within various commercial branches, and within specific industries. (AB)
[en] Major trends in the natural gas industry, including deregulation and the impact of natural gas commodity competition on consumers, and SaskEnergy's current and historical residential rate structures were reviewed. The object of the exercise was to provide the Utility (i.e. SaskEnergy) with independent advice on the proposed changes to rate structures and billing practices for residential customers in Saskatchewan. It was concluded that SaskEnergy 's historical and current rate methodologies are consistent with generally accepted practices in the natural gas industry. The proposed changes to rate structures and billing practices to unbundle the gas commodity from delivery services is an important step in the evolution of competition in the supply of natural gas. Customers will not only have more choice in terms of supplier, they will be able to have a better understanding of both the impact of volatile commodity prices and any changes in the cost of delivery services on rates charged by SaskEnergy
[en] We present a method for self-assembling arrays of live single cells on a glass chip using a photopatternable polymer to form micro-traps. We have studied the single-cell self-assembly method and optimized the process to obtain a 52% yield of single-trapped cells. We also report a method to measure the oxygen consumption rate of a single cell using micro-patterned sensors. These molecular oxygen sensors were fabricated around each micro-trap allowing optical interrogation of oxygen concentration in the immediate environment of the trapped cell. Micromachined micro-wells were then used to seal the trap, sensor and cell in order to determine the oxygen consumption rate of single cells. These techniques reported here add to the collection of tools for performing 'singe-cell' biology. An oxygen consumption rate of 1.05 ± 0.28 fmol min−1 was found for a data set consisting of 25 single A549 cells.
[en] It is necessary to establish the approaches that should be involved to the sustainability concept, clearly of agreement with the concrete objectives that it wants to reach (sustainable agriculture, economic, institutional sustainability, politics) as well as to evaluate and to discern the varied interpretations on the methodologies of sustainability determination. The urgency of understanding like an integrality the development problems in the countries of the third world, related with their, agricultural sectors, the state and potentiality of their resources, the exchange relationships in the international market and the raw materials, allow the presentation of different roads for the identification of strategies in the search of the sustainability