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[en] Eight modifications of the ''universal'' medium are proposed, differing one from another by the method of preparing glucose. Thioglycolane and Saburo media were used as controls. All experiments were conducted in Petri dishes. It has been found that the ''universal'' medium can be improved by increasing glucose up to 1% and lowering sterilization temperature to 1080C (for glucose-enriched media). The ''universal'' medium was then compared with other media by calculating ID50 (50% infectivity dose). The results obtained for S.faecium A2I,B.pumilus E601, Bsphaericus C1A, B.subtilis 6633, S.aureus 209P, C.albicans L-45, and Cl.sporogenes 477 have confirmed the suitability of the ''universal'' medium for use in radiosterilization studies.(author)
[en] The feasibility of glycosylation post-purification has been demonstrated by introducing glucose into the model protein lysozyme via a novel reaction that is compatible with biological samples. The crystallization of glycoproteins is one of the challenges to be confronted by the crystallographic community in the frame of what is known as glycobiology. The state of the art for the crystallization of glycoproteins is not promising and removal of the carbohydrate chains is generally suggested since they are flexible and a source of heterogeneity. In this paper, the feasibility of introducing glucose into the model protein hen egg-white lysozyme via a post-purification glycosylation reaction that may turn any protein into a model glycoprotein whose carbohydrate fraction can be manipulated is demonstrated
[en] Oxidation of glucose oxidase by ferrocenecarboxylic acid in the presence of glucose was examined by comparing experimental catalytic efficiencies from cyclic voltammetry with those digitally simulated by the general program ELEC. The program permitted consideration of individual diffusion coefficients of enzyme and mediator, the second-order nature of the initial electron-transfer event and the follow-up reaction of oxidized enzyme with glucose. A rate constant of 1.06x105 1 mol-1 s-1 was found by analysis with such simulations for the accepted two-electron EC'C pathway. This was nearly half the approximate value found by a previously used pseudo-first-order EC' model assuming equal diffusion coefficients. (author). 15 refs.; 3 figs.; 1 tab
[en] Natural killer/T-cell (NK/T-cell) lymphoma is a rare condition, which presents as necrotic, granulomatous lesions involving the nose and the upper respiratory tract. The condition usually has an aggressive clinical course. The predominant subtype of NK/T-cell lymphoma noted in Asian population is the nasal type. We describe a case of biopsy-proven NK/T-cell lymphoma with bilateral adrenal involvement. Adrenal involvement by lymphoma is usually of B-cell type and occurs in disseminated disease and often unilateral. Bilateral adrenal involvement by T-cell lymphoma is extremely rare
[en] Highlights: • Ocimum tenuiflorum leaf extract and silver nitrate were used to prepare Ag NPs. • Structural, optical and morphological properties of Ag NPs were studied. • The GCE modified with Ag NPs showed a sensitivity of 895.8 μAmM−1cm−2. • Bio-mediated synthesized NPs showed sustainable glucose sensing properties.
[en] N-acetylglucosaminyl transferase(GnT) is related to the development of tumor and the cancer patients' prognosis by effecting the change of glucose's chain. Study on the transform of glycosyltransferase is benefit to the comprehension of the mechanism of biological behavior. The noninvasive diagnostic and treating methods of tumor will be provided along with the development of new imaging agent of tumor glucose analogue and its mechanism defined clearly. (authors)
[en] Here in this paper we report an IR-IR double resonance study of the structural landscape present in the Na+(glucose) complex. Our experimental approach involves minimal modifications to a typical IR predissociation setup, and can be carried out via ion-dip or isomer-burning methods, providing additional flexibility to suit different experimental needs. In the current study, the single-laser IR predissociation spectrum of Na+(glucose), which clearly indicates contributions from multiple structures, was experimentally disentangled to reveal the presence of three α-conformers and five β-conformers. Comparisons with calculations show that these eight conformations correspond to the lowest energy gas-phase structures with distinctive Na+ coordination.
[en] Total knee arthroplasty has witnessed a significant increase in recent years. Despite the advantages of this surgical procedure, it has some complications, the most serious of which is prosthetic infection. The discrimination of bacterial infections from sterile inflammatory processes is of great importance in the management of periprosthetic infection (PPI). Ubiquicidin (UBI) is a synthetic antimicrobial peptide fragment reported to be highly infection-specific. Tc99m-UBI has recently been reported to be a promising radiotracer for infection imaging. We report a case of left knee PPI diagnosed using 99mTc-UBI scintigraphy and compared with F-18 fluorodeoxy-glucose positron emission tomography