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[en] Ellagic acid (EA) has been gaining a considerable attention in recent years. The interest in non-drug and herbal base therapies is being increased. EA is a biological molecule found in different fruits and seeds, which is known to have the ability to scavenge reactive oxygen radicals. It has been observed that methyl tert-butyl ether (MTBE) has destructive effects on structure and function of hemoglobin (Hb), an important respiratory blood protein. This conclusion is reached from our far-UV circular dichroism, Soret band absorption, fluorescence and oxygen affinity measurements. It has also been observed from our chemiluminscence measurements that ROS production is increased in the presence of MTBE which degrades heme in Hb. The main goal of this study was to offer a way to scavenge ROS produced during MTBE interaction with Hb. We report that EA decreased the heme degradation and ROS production in Hb solutions containing MTBE. - Highlights: • Hemoglobin structure and function were disturbed in the presence of methyl tert-butyl ether (MTBE). • Heme degradation was increased due to production of reactive oxygen specious. • Deleterious effects of reactive oxygen specious were reduced by ellagic acid.
[en] Background: Acrylamide-induced immunotoxicity and allergic dermatitis have been reported in animal experiments and clinical reports, respectively. However, epidemiological evidence from the general population is limited. Objectives: The purpose of the present study was to estimate the associations between acrylamide exposure and allergy-related outcomes in the general US population. Methods: A total of 6982 subjects were selected from the National Health and Nutrition Examination Survey 2005–2006 (NHANES). Internal exposure was measured by the hemoglobin adducts of acrylamide (HbAA) and its metabolite glycidamide (HbGA). Allergy-related outcomes including asthma, hay fever, allergy, itchy rash, sneeze, wheeze and eczema were obtained by self-administered questionnaires. Allergic sensitization was assessed by the total immunoglobulin E (IgE) levels. The associations of HbAA and HbGA quartiles with allergy-related outcomes were calculated using logistic regression models with multivariable adjustments. Analyses were additionally stratified according to age, gender and serum cotinine levels. Results: When setting quartile 1 of HbAA as reference, the odds ratios (ORs) [95% confidence intervals (CIs)] of quartile 2 to 4 for eczema were 1.18 (0.79–1.76), 1.14 (0.73–1.78) and 1.58 (1.14–2.18), respectively (ptrend = 0.002). Individuals at the highest quartile of HbGA had significantly elevated likelihoods of itchy rash (OR = 1.37, 95% CI = 1.02–1.83, ptrend = 0.032) and eczema (OR = 1.45, 95% CI = 1.06–1.97, ptrend = 0.044). The stratification analyses indicated various results in different subgroups. Conclusions: This study indicated significant associations between HbAA and HbGA levels and the likelihoods of allergy-related outcomes in the general US population, depending on age, gender and smoke exposure status. These findings suggested potential public health concerns for the widespread exposure to acrylamide. - Highlights: • Health effects of acrylamide and glycidamide on allergy-related outcomes were assessed in the general US population. • Elevated HbAA levels are associated with increased likelihoods of eczema. • Elevated HbGA levels are associated with increased likelihoods of itchy rash and eczema. • Associations between HbAA and HbGA levels and allergy-related outcomes varied by age, gender and serum cotinine levels. - Levels of HbAA and HbGA are significantly associated with allergy-related outcomes in the general US population.
[en] We prepared silica nanospheres 360 nm in diameter surface-modified with p-tert-butylthiacalixarenes containing amine, carboxyl, and guanidinium groups. We found that these silica nanoparticles selectively adsorb model oligonucleotides and proteins. The particles modified with the macrocycle containing guanidinium fragments selectively adsorbed long-chain oligonucleotides and those modified with the macrocycle containing amine groups adsorbed BSA and hemoglobin with pH-dependent selectivity. We compared this behavior with that of silica nanoparticles carrying amine and carboxyl groups, and concluded that both electrostatic interactions and specific binding are responsible for the observed selectivity
[en] Although nicotinamide administration has increased the radiosensitivity of experimental tumors, there is a scarcity of data detailing the underlying physiological mechanisms. The current study presents a method for quantifying both microregional distributions of intravascular HbO2 saturations and the presence or absence of blood flow in adjacent frozen tumor sections. Two murine tumor cell lines, KHT and SCCVII, were implanted and quick-frozen without the use of anesthetics. Nicotinamide was administered IP 1 h prior to freezing, and a fluorescent dye that preferentially stains cells adjacent to blood vessels was injected IV 1 min prior to freezing. To visualize the presence or absence of blood flow, six micron sections were first cut using a cryostat. The remaining frozen tumor block was then analyzed cryospectrophotometrically to determine intravascular HbO2 levels. While KHT HbO2 levels increased somewhat predictably following nicotinamide, the response in SCCVII tumors varied with distance from the tumor surface. Near the periphery, SCCVII HbO2 levels increased, but nearer the tumor center, HbO2 levels actually decreased. Perfused blood vessels were uniformly distributed throughout the tumor volume except in regions of necrosis. Even vessels containing no measurable oxygen remained perfused, as evidenced by the presence of the fluorescent marker. These results demonstrate that nicotinamide raises intravascular HbO2 saturations in both KHT and SCCVII tumors. This increase in oxygen delivery is not evenly distributed throughout the tumor volume in spite of a uniform distribution of perfused blood vessels. Blood flow in a substantial proportion of these vessels is most likely not sufficiently rapid to serve a functional purpose in terms of oxygen supply to the surrounding tumor tissue. 15 refs., 4 figs
[en] Malaria has protean clinical manifestations and renal complications, particularly acute renal failure that could be life threatening. To evaluate the incidence, clinical profile, ou come and predictors of mortality in patients with malarial acute renal failure, we retrospectively studied the last two years records of malaria induced acute renal failure in patients with peripheral smear positive for malarial parasites. One hundred (10.4%) (63 males, 37 females) malaria induced acute renal failure amongst 958 cases of acute renal failure were evaluated. Plasmodium (P). falciparum was reported in 85%, P. vivax in 2%, and both in 13% patients. The mean serum creatinine was 9.2 ± 4.2 mg%, and oligo/anuria was present in 82%; 78% of the patients required hemodialysis. Sixty four percent of the patients recovered completely, 10% incompletely, and 5% developed chronic kidney failure; mortality occurred in 21% of the patients. Low hemoglobin, oligo/anuria on admission, hyperbilirubinemia, cerebral malaria, disseminated intravascular coagulation, and high serum creatinine were the main predictors of mortality. We conclude that malaria is associated with acute renal failure, which occurs most commonly in plasmodium falciparum infected patients. Early diagnosis and prompt dialysis with supportive management can reduce morality and enhance recovery of renal function (Author).
[en] In this paper the strength and elasticity model of hemoglobin blood structure has been studied. It is established that the elasticity model of blood hemoglobin is equal to E=2·104 H/m2. This value is exactly correspond with the value of osmolarity of hemoglobin molecule in erythrocyte. (author)
[en] The prognostic significance of intratumour microvessel density (IMD) and haemoglobin (Hb) level was studied in 152 (FIGO stage IB -IIIB) cervical cancer patients before radiotherapy. Patients' age and tumour stage, grade and degree of keratinisation were also studied. IMD measurement expressed as the mean vessel count per 1 mm2 was performed on formalin-fixed, paraffin-embedded tumour biopsies stained with anti-factor VIII antibody using immunohistochemistry and the vascular hot-spot technique. The median age of patients was 55 years (29-80). Median values for IMD and Hb level were: 142.5 (range 56.3-476.6) vessels/mm2 and 129 (range 81-160) g/l, respectively. The median time of follow-up was 26 months, with a range of 2-145 months. Tumour stage (p=0.7009), grade (p=0.6660) and degree of keratinisation (0.2669) were not significant in the Kaplan-Meier univariate analysis. However, patients' age >50 years (p=0.0079), high vascularity (IMD >190.0 vessels/mm2, minimal cut-off point, p=0.0503) and Hb concentration >116 g/l (p=0.0213) were favourable prognostic factors for cervical cancer treated with radiotherapy. In the Cox multivariate analysis only higher vascularity (IMD>190/mm2 and Hb concentration >116 g/l were favourable prognostic factors in terms of patients' survival. However, when a Cox analysis was done separately for keratinizing and non-keratinizing tumours, it was found that higher vascularity was significant only for keratinizing, and higher Hb level only for non-keratinizing cancers
[en] This study aimed to evaluate the usefulness and outcomes of stent graft use in dysfunctional arteriovenous grafts. Eleven patients who underwent stent graft placement for a dysfunctional hemodialysis graft were included in this retrospective study. Expanded polytetrafluoroethylene covered stent grafts were placed at the venous anastomosis site in case of pseudoaneurysm, venous laceration, elastic recoil or residual restenosis despite the repeated angioplasty. The patency of the arteriovenous graft was evaluated using Kaplan-Meier analysis. Primary and secondary mean patency was 363 days and 741 days. Primary patency at 3, 6, and 12 months was 82%, 73%, and 32%, respectively. Secondary patency at the 3, 6, 12, 24, and 36 months was improved to 91%, 82%, 82%, 50%, and 25%, respectively. Fractures of the stent graft were observed in 2 patients, but had no effect on the patency. Stent graft placement in dysfunctional arteriovenous graft is useful and effective in prolonging graft patency