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[en] Triptolide is the predominant active component of the Chinese herb Tripterygium wilfordii Hook F (TwHF) that has been widely used to treat several chronic inflammatory diseases due to its immunosuppressive, anti-inflammatory, and anti-proliferative properties. In the present study, we elucidated the cardioprotective effects of triptolide against cardiac dysfunction and myocardial remodeling in chronic pressure-overloaded hearts. Furthermore, the potential mechanisms of triptolide were investigated. For this purpose, C57/BL6 mice were anesthetized and subjected to transverse aortic constriction (TAC) or sham operation. Six weeks after the operation, all mice were randomly divided into 4 groups: sham-operated with vehicle group, TAC with vehicle group, and TAC with triptolide (20 or 100 μg/kg/day intraperitoneal injection) groups. Our data showed that the levels of NLRP3 inflammasome were significantly increased in the TAC group and were associated with increased inflammatory mediators and profibrotic factor production, resulting in myocardial fibrosis, cardiomyocyte hypertrophy, and impaired cardiac function. Triptolide treatment attenuated TAC-induced myocardial remodeling, improved cardiac diastolic and systolic function, inhibited the NLRP3 inflammasome and downstream inflammatory mediators (IL-1β, IL-18, MCP-1, VCAM-1), activated the profibrotic TGF-β1 pathway, and suppressed macrophage infiltration in a dose-dependent manner. Our study demonstrated that the protective effect of triptolide against pressure overload in the heart may act by inhibiting the NLRP3 inflammasome-induced inflammatory response and activating the profibrotic pathway. - Highlights: • Chronic pressure overload increases expression of NLRP3 inflammasome in the heart. • Triptolide attenuates chronic pressure overload-induced myocardial remodeling. • The mechanism appears to involve inhibition of NLRP3 inflammasome expression. • Triptolide is a therapeutic candidate for hypertensive heart disease.
[en] In this study, the inhibited effects on superoxide radical of Grifola umbellata hot water extracts (Gu-extract) on mice were investigated. The intraperitoneal administration of Gu-extract for 2 days increased swimming time by 1.8 fold, indicating that the extract might contained some anti-fatigue compound (s). The extract inhibited superoxide radical formation by 22% as compared to control, but not lipid peroxidation
[en] Tritiated water 3H, injected by intraperitoneal route into 7-week old male Rats, was incorporated into lipids synthesized de novo. The Rats were killed 0, 3, 7, 10, 15, 30, 60 and 120 min. after tracer injection. The results show that an optimal interval of about 10 min. between tracer injection and animal sacrifice was necessary to obtain a correct estimate of lipogenesis de novo by avoiding intertissue exchanges
[fr]L'incorporation, dans les lipides synthetises de novo, du 3H de l'eau tritiee injectee par voie intraperitoneale a ete etudiee chez le Rat male age de 7 semaines. Les animaux ont ete sacrifies 0, 3, 7, 10, 15, 30, 60 et 120 mn apres l'injection du traceur. Les resultats indiquent qu'une duree de l'ordre de 10 mn entre l'injection du marqueur et le sacrifice des animaux est la plus convenable pour apprecier correctement la lipogenese de novo, en evitant les echanges intertissulaires
[en] Complete text of publication follows. Redox relating metals such as Cu, Fe and Zn accumulate in tissues by aging, and produce free radicals. In addition, it is known that Cu accumulation in liver is related to hepatocarcinogenesis. We tried to reduce Cu content in liver by using metal chelators. Either thiosalicylate or disulfiram injected mouse intraperitoneally. And Cu, Zn, Fe, Ca, and Mg contents in liver were measured 24 hours after the injection. When 25, 50, or 100 mg/kg of disulfiram was injected, no metal contents were affected. However, Cu content decreased in a dose-dependent manner after the injection of thiosalicylate while the contents of the other four metals did not change. Then, the liver Cu content was observed time dependently after 5, 10, and 25 mg/kg of thiosalicylate were intraperitoneally injected and found that Cu content was reduced up to 5 hours depending on the dose. From the present results, it is speculated that thiosalicylate may be used as a therapeutic agent which removes copper from liver tissues.
[en] Objective: To assess the functional status and quality of life (QOL) of 12 patients with disseminated peritoneal cancer before and after intraperitoneal chemotherapy (IPCT). Design: Patients with confirmed diagnosis of malignant ascites originating from gastrointestinal tract, gynecologic or other malignancies with peritoneal implants were enrolled in this study. Twelve patients completed the Functional Assessment of Cancer therapy scale to assess quality of life and the Visual Analogue scale to assess common symptoms before and after palliative abdominal paracentesis with intraperitoneal chemotherapy (IPCT). Results: Twelve patients enrolled, 8 gastrointestinal, 3 gynecologic malignancies and 1 with pseudomyxoma peritonie. All with terminal advanced stage malignancy. Mean number of days required for palliative abdominal paracentesis before (IPCT) was 6.7 days (3-20 days) while mean number of days after IPCT was 18.9 days (10-35 days). Eleven out of 12 patients had relief of their symptoms and improvement in QOL. Side effects were minimal and tolerable. Conclusion: Intraperitoneal chemotherapy improves QOL and increase the duration required for palliative abdominal paracentesis for terminal cancer patients with malignant ascites with minimal toxicity. (author)
[en] To assess the efficacy of large volumes of diluted intraperitoneal bupivacaine in post-laparoscopic cholecystectomy analgesia. Study Design: A randomised controlled trial. Place and Duration of Study: Department of General Surgery, Nishtar Hospital, Multan, from August 2018 to June, 2019. Methodology: Two equal groups with 55 patients each were formed. Normal saline 500 ml in group A, and mixture of 20 ml 0.5% bupivacaine in 480 ml normal saline in group B, was used to irrigate peritoneal cavity. Final outcome of the study was the comparison of pain-free duration. Postoperatively, numerical rating scale (NRS) score at various intervals and total analgesics requirement within 24 hours after the procedure were included in the secondary outcomes. Student's t-test was applied on continuous data and Pearson’s Chi-square test on nominal variables. P >0.05 was considered of no statistical significance. Results: Both groups were comparable for age, weight, gender, duration of surgery. Postoperative analgesia duration was 0.99 ±0.51 hours in group A and 16.53 ±2.65 hours in group-B (p<0.001). On average, 124.80 ±26.68 mg and 31.00 ±14.98 mg tramadol was given to group A and B patients, respectively (p<0.001). There was statistically significant difference in NRS score at 30 minutes, 1, 3, 6 and 12 hours postoperatively (p<0.05). NRS score at ETT extubation and at 24 hours was statistically not different (p >0.05). Conclusion: Large volume of diluted bupivacaine when injected intraperitoneally during laparoscopic cholecystectomy provides prolonged time pain relief. (author)
[en] The urachus establishes a connection between the dome of the bladder and the umbilicus throughout fetal life. If the urachus does not close completely, malignancy is a potential complication. The primary treatment for malignant urach al tumor is surgical excision. A 61-year-old male patient diagnosed with urach al carcinoma had undergone partial cystectomy 25 years previously. Twenty years later, local recurrence was treated with another partial cystectomy without umbilical remnant excision. Recurrence at the umbilical site was excised 2 years later, but intraperitoneal invasion had occurred, and the patient underwent a total cyclamate at that time. Local disease and disseminated metastases in the thorax and intra- and extra peritoneal areas were noted upon admission to our hospital. Urach al carcinomas are usually aggressive tumors, and surgical treatment should include partial or radical cystectomy and excision of the urachus and umbilicus, to prevent local recurrence and distant metastasis