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AbstractAbstract
[en] The preliminary steps for a radio-immunoassay of Thyrotropin Releasing Hormone (TRH) are described. Rabbit antiserum at dilution 1:10000 is used with radioiodinated TRH (125I). It is possible to assay from 5 to 1000 pg unlabeled TRH with an intraassay reproducibility varying from 7 to 4% and the lowest detectable amount in this system is 10 pg TRH. TRH mean and standard deviation in normal subjects are 136,9 and 52,9 pg/ml
[fr]
On a decrit les resultats preliminaires d'un dosage radioimmunologique de la Thyrotropin Releasing Hormone (TRH). On a utilise un antiserum de Lapin a la dilution 1:10000 avec de la TRH marquee a l'iode 125. L'etendue de la gamme de dosage couvre l'intervalle de 5 a 1000 pg de TRH froide. La reproductibilite intradosage varie entre 7 et 4% et la sensibilite du systeme est de 10 pg. La moyenne et l'ecart-type obtenus avec un echantillon de 72 sujets normaux sont respectivement de 136,9 et 52,9 pg/mlOriginal Title
Dosage radioimmunologique de la thyrotropin releasing hormone
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Journal Article
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Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales; v. 171(6); p. 1216-1220
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AbstractAbstract
[en] Proteoglycans (PG) have been purified by classical methods from human articular cartilage in order to set up a radioimmunoassay. Conditions of labelling, purification of labelled PG, and optimal conditions of buffer, temperature, duration of incubations and dilution of antiserum are described. Separation of free and bound PG is performed by immunoprecipitation. It is demonstrated that human articular PG can be assayed quantitatively by RIA procedure, with a sensitivity of +- 2 femto-moles (+- 5 ng) per tube
[fr]
Les proteoglycannes (PG) cartilagineux humains de nature articulaire, isoles et purifies par la methode classique, font l'objet de la mise au point d'un dosage radio-immunologique. Les conditions d'obtention d'anticorps chez l'animal, de marquage de l'antigene a l'125I et la purification des produits de marquage par chromatographie sont decrites. La fixation de l'antigene a l'anticorps est etudiee en fonction du temps a differentes temperatures d'incubation. La separation de PG marque libre du complexe PG marque-anticorps est obtenue par double anticorps en presence de NaCl 0,15 M. La sensibilite du dosage est de +- 2 femto-moles (5 ng PG) par tube utilisant une dilution de l'antiserum a 1/10000Original Title
Purification de proteoglycannes cartilagineux humains et aspects techniques du dosage radioimmunologique
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Journal Article
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Comptes Rendus des Seances de la Societe de Biologie et de ses Filiales; v. 174(5); p. 867-877
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AbstractAbstract
No abstract available
Original Title
La densitometrie osseuse par la methode de Cameron dans la surveillance des osteoporoses et des malades en hemodialyse chronique
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Journal Article
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J. Radiol., Electrol., Med. Nucl; v. 55(4); p. 333-334
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AbstractAbstract
No abstract available
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8. Annual meeting of the European Thyroid Association; Lyon, France; 27 - 30 Sep 1977; Published in abstract form only.
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Journal Article
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Conference
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Annales d'Endocrinologie (Paris); v. 38(5); p. 3A
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Baret, A.; Michel, P.; Broussolle, B.
Centre de Recherches du Service de Sante des Armees. 1979 Scientific works1980
Centre de Recherches du Service de Sante des Armees. 1979 Scientific works1980
AbstractAbstract
No abstract available
Original Title
Dosage radioimmunologique des superoxydes dismutases
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Centre de Recherches du Service de Sante des Armees, 92 - Clamart (France); 446 p; Jul 1980; p. 267-271; Published in summary form only.
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Report
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Progress Report
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AbstractAbstract
[en] Short communication
Source
Israel Atomic Energy Commission, Tel Aviv (Israel). Licensing Div; 279 p; Jul 1993; p. 142
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Maclouf, J.; Renou, M.; Pradelles, P.; Dray, F.; Nehr, C.
17. French language symposium on nuclear medicine. Paris, June 2-4, 19751975
17. French language symposium on nuclear medicine. Paris, June 2-4, 19751975
AbstractAbstract
[en] In radioimmunological analysis using tritiated tracers the specific activity is limited for certain commercial products, prohibiting the determination of compounds such as PGE(I) (110Ci/mmole which has been found at a concentration below 2pg/ml in human peripheral plasma. An attempt was made to see whether this problem could be solved by the use of iodinated PG of much higher specific activity. The method employed is described, and shows a greatly improved sensitivity in the radioimmunological analysis of the PG tested owing to the use of an iodinated (iodine 125) tracer. In the case of PGE(I) for instance the sensitivity was increased by a factor 25
[fr]
Dans le dosage radioimmunologique a l'aide de traceurs trities, l'activite specifique reste limitee pour certains produits commerciaux, ne permettant pas le dosage de composes tels que PGE(I) (110Ci/mmole) dont on a montre une concentration inferieure a 2pg/ml dans le plasma peripherique humain. On a recherche si l'emploi de PG iodees d'activite specifique nettement superieure pouvait resoudre ce probleme. On a decrit la methode employee. Elle a permis de constater que la sensibilite du dosage radioimmunologique des PG testees a ete nettement amelioree grace a l'utilisation d'un traceur iode (iode 125). Dans le cas des PGE(I) par exemple, on a obtenu une sensibilite accrue de 25 fois avec le traceur iodeOriginal Title
Utilisation de derives iodes pour le dosage radioimmunologique des prostaglandines
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Source
Kellershohn, C.; Raynaud, C. (eds.); CEA Centre d'Etudes Nucleaires de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Biologie; p. 156-158; ISBN 2727200013;
; 1975; 17. French language symposium on nuclear medicine; Paris, France; 02 Jun 1975

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AbstractAbstract
No abstract available
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Israel Atomic Energy Commission, Tel Aviv; p. 237-239; Apr 1980; p. 237-239; Published in summary form only.
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AbstractAbstract
[en] A conjugate was obtained by covalent bond coupling of penicilloyl groups to bovine serum albumine (BSA) previously labelled with iodine 125. The reaction takes place in alkaline solution without intervention of carbodiimide. By its immunoreactivity and specific activity this conjugate is a suitable tracer in radioimmunology. The sensitivity and specificity of the determination were analysed on standard curves
[fr]
On a obtenu un conjugue en couplant par liaison covalente des groupements penicilloyl a de la serum albumine bovine (BSA) prealablement marquee a l'iode-125. La reaction est realisee en milieu alcalin, sans intervention de carbodiimide. L'immunoreactivite et l'activite specifique de ce conjugue en font un traceur utilisable en radioimmunologie. On a analyse sur des courbes standard la sensibilite et la specificite du dosageOriginal Title
Utilisation d'un derive iode de la penicilline pour le dosage radioimmunologique des groupements penicilloyl dans les milieux biologiques
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Source
Kellershohn, C.; Raynaud, C. (eds.); CEA Centre d'Etudes Nucleaires de Saclay, 91 - Gif-sur-Yvette (France). Dept. de Biologie; p. 159-161; ISBN 2727200013;
; 1975; 17. French language symposium on nuclear medicine; Paris, France; 02 Jun 1975

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AbstractAbstract
[en] For the treatment of cancer, the therapeutic potential of short-range, low-energy Auger-electron emitters, such as 125I, is getting progressively wider recognition. The potency of Auger-electron emitters is strongly dependent on their location in close vicinity to DNA. We have developed a new two-step targeting strategy to transport 125I into cancer-cell nuclei using PEG-stabilized tumour-cell targeting liposomes named ''Nuclisome-particles''. In the present study, epidermal growth factor (EGF) was used as a tumour-cell-specific agent to target the EGF-receptor (EGFR) and the liposomes were loaded with 125I-Comp1, a recently synthesized daunorubicin derivative. As analysed with cryo-TEM, the derivative precipitates inside liposomes at a drug-to-lipid molar ratio of 0.05:1. Receptor-specific uptake in cultured U-343MGaCl2:6 tumour cells of EGFR-targeting liposomes increased with time while non-specific and receptor-blocked uptake remained low. Nuclisome-particles were able to target single U-343MGaCl2:6 cells circulating in human blood during 4 h, with low uptake in white blood cells, as demonstrated in an ex vivo system using a Chandler loop. Autoradiography of targeted cells indicates that the grains from the radiolabelled drug are mainly co-localized with the cell nuclei. The successful targeting of the nucleus is shown to provide high-potency cell killing of cultured U-343MGaCl2:6 cells. At the concentration used, Nuclisome-particles were up to five orders of magnitude more effective in cell killing than EGFR-targeting liposomes loaded with doxorubicin. The results thus provide encouraging evidence that our two-step targeting strategy for tumour cell DNA has the potential to become an effective therapy against metastasizing cancer cells in the bloodstream. (orig.)
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Available from: http://dx.doi.org/10.1007/s00259-009-1225-7
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Journal Article
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European Journal of Nuclear Medicine and Molecular Imaging; ISSN 1619-7070;
; v. 37(1); p. 114-123

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