Filters
Results 1 - 10 of 41002
Results 1 - 10 of 41002.
Search took: 0.048 seconds
Sort by: date | relevance |
AbstractAbstract
[en] Human placental β-hexosaminidase A was labeled with 125iodine to high specific activity with the retention of conformational integrity as judged by the retention of enzymatic activity. The oligosaccharide structure also appeared to be intact since the labeled enzyme was cleared from the circulation of the rat with a half-life identical to that of the unlabeled enzyme and an excess of unlabeled enzyme effectively blocked the clearance of the labelled form. Furthermore, the pattern of inhibition of clearance of the native and labeled enzymes by asialofetuin and mannans was identical. The useful and mild procedure for labeling enzymes may be of general importance in the preparation of enzymes for metabolic studies in normal animals and animal models of genetic lysosomal storage disorders. (Auth.)
Record Type
Journal Article
Journal
Clinica Chimica Acta; ISSN 0009-8981;
; v. 97(2-3); p. 155-158

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] The labelling technics have been a big support for the advances reached in Biology Research. The use of radioisotopes has been essential for these technics, but alternative nonradioactive labelling technics have developped during the last few years. These nonradioactive technics are as sensitive and accurate as the radioactive ones, but without irradiation risk. This article report presents a short review about both types of technics, explaining their basis, making comparisons between them and discussing about the advantages and disadvantages associated to them
Original Title
Tecnicas sustituivas a la utilizacion de radioisotopos marcadores en investigacion biologica
Primary Subject
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] This volume contains the typescript texts of six plenary lectures plus short (ca. 2 pp) versions of a very large number of papers from the Second International Symposium, held in Kansas City in 1985; author and subject indexes
Primary Subject
Source
1986; 542 p; Elsevier Science Publishers; Amsterdam (Netherlands); From review in J. Am. Chem. Soc., Vol. 109, No. 24 (1987).
Record Type
Book
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] The juvenoid analog 2-(4-(ethoxycarbamato)ethoxy)benzyl)-1-cyclohexane ethylene acetal (W 328) was labelled with 3H in the benzyl position by the CESG method. 3H-NMR revealed the stereoselectivity of the labelling. The results are compared with data published in the literature and discussed in the terms of stereoelectronic requirements. (author) 2 figs., 7 refs
Record Type
Journal Article
Journal
Collection of Czechoslovak Chemical Communications; ISSN 0010-0765;
; CODEN CCCCAK; v. 58(3); p. 1164-1168

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] At the - Departement d'Ingenierie et d'etudes de proteines (Deip) of the CEA more than seventy researchers are working hard to understand the function of proteins. For that they use the molecular labelling technique (F.M.)
Original Title
Ingenierie des proteines
Primary Subject
Secondary Subject
Source
Association des Ingenieurs en Genie Atomique du Maroc, Rabat (Morocco). Funding organisation: Association des Ingenieurs en Genie Atomique du Maroc, Rabat (Morocco); 36 p; 11 Mar 2000; p. 12; Information day on nuclear techniques for life sciences; Journee d'information sur les techniques nucleaires au service des sciences du vivant; Rabat (Morocco); 11 Mar 2000; Available from Association des Ingenieurs en Genie Atomique du Maroc, Rabat (MA)
Record Type
Miscellaneous
Literature Type
Conference
Country of publication
Reference NumberReference Number
Related RecordRelated Record
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
No abstract available
Source
Short communication only.
Record Type
Journal Article
Journal
Journal of Chromatography; ISSN 0021-9673;
; v. 189(3); p. 433-435

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
No abstract available
Original Title
Produccion de moleculas marcadas en la Facultad de Quimica de la UNAM
Source
18. Mexican Congress of Pure and Applied Chemistry; Coatzacoalcos (Mexico); 11-15 Oct 1983; Published in summary form only.
Record Type
Journal Article
Literature Type
Conference
Journal
Revista de la Sociedad Quimica de Mexico; ISSN 0583-7693;
; v. 27(5); p. 291

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
Melo e Silva, M.C.M. Cristina; Patricio, Luciana; Gano, Lurdes; Sa e Melo, M. Luisa; Inohae, Eiko; Mataka, Shuntaro; Thiemann, Thies, E-mail: lucianap@itn1.itn.pt2001
AbstractAbstract
[en] The synthesis of two novel radiolabelled estrogen derivatives, [125I](E)-3-methoxy-17α-iodovinylestra-1,3,5(10),6-tetraen-17β-ol (E[125I]IVDE) and [125I](Z)-3-methoxy-17α-iodovinylestra-1,3,5(10),6-tetraen-17β-ol (Z[125I]IVDE), was carried out aiming to study the influence of the introduction of a C6-C7 double bond on the biological properties of the estradiol molecule. 3-Methoxyestra-1,3,5(10),6-tetraen-17-one was synthesised starting from a suitably protected estrone and subsequently converted into the 17α-ethynyl derivative. The radioiodinated derivatives were stereoselectively formed by radioiododestannylation of the corresponding tributylstannyl precursors. The biodistribution of the novel [125I]iodovinylestradiol derivatives was evaluated in immature female mice. Biological data indicated that the Z-isomer, owing to its higher in vivo uptake by the target tissue, has the preferable configuration for further development of similar compounds for estrogen receptor detection.
Primary Subject
Source
S0969804399002584; Copyright (c) 2001 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; This record replaces 33037053; Country of input: Belgium
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] Coordination chemistry of technetium 99 applied to biology and medicine. Rational conceptualisation of new photon emitter probes for in vivo investigations with technetium 99m (on the tracer scale) requires the coordination chemistry of ponderable technetium 99 isotope. Physicochemical and structural studies carried out on technetium 99 complex models enable us to identify predictive parameters involved in radiopharmaceutical in vivo tropism (specificity) for the target bodies. In addition, this work leads to the development and control of a new technetium complexation protocol under physiological conditions (pH ∼ 7.4 and T < 45 deg. C) in order to label bio-vectors without denaturing them. (author)
Original Title
Application de la chimie de coordination du technetium 99 dans le domaine de la biologie et de la medecine
Primary Subject
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] α-Cyano-3-phenoxybenzyl 2, 2, 3, 3-tetramethylcyclopropanecarboxylate (fenpropathrin), a new potent synthetic pyrethroid, was labelled with carbon-14 at the cyclopropyl group for studies on its metabolism in mammals, fishes, plants and soils. Condensation of potassium cyanide-14C (2) with 1,3-diphenyl-2-thiourea (3) in the presence of basic lead carbonate gave 1-cyano-14C-N,N'-diphenylformamidine (4). Reduction of 4 followed by hydrolysis with barium hydroxide afforded glycine-2-14C (6). Esterification of 6 with ethanol in the presence of anhydrous hydrogen chloride gave ethyl glycinate-2-14C hydrochloride (7). Diazotization of 7 followed by condensation with 2, 3-dimethyl-2-butene yielded ethyl 2, 2, 3, 3-tetramethylcyclopropane-1-14C-carboxylate (8). Hydrolysis of 8 gave 2, 2, 3, 3-tetramethylcyclopropane-1-14C-carboxylic acid (9), which was esterified with α-cyano-3-phenoxybenzyl bromide (10) to afford fenpropathrin(cyclopropyl-1-14C) (1) in 13 % yield from 2. (author)
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
1 | 2 | 3 | Next |