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[en] Shifts in terminal electron-accepting processes during biostimulation of uranium-contaminated sediments were linked to the composition of stimulated microbial populations using DNA-based stable isotope probing. Nitrate reduction preceded U(VI) and Fe(III) reduction in [13C]ethanol-amended microcosms. The predominant, active denitrifying microbial groups were identified as members of the Betaproteobacteria, whereas Actinobacteria dominated under metal-reducing conditions.
[en] This study evaluates the effects of the triazine herbicide simazine in an outdoor pond microcosm test system that contained two submerged rooted species (Myriophyllum spicatum and Elodea canadensis) and two emergent rooted species (Persicaria amphibia and Glyceria maxima) over a period of 84 days. Simazine was applied to the microcosms at nominal concentrations of 0.05, 0.5 and 5 mg/L. General biological endpoints and physiological endpoints were used to evaluate herbicide toxicity on macrophytes and the algae developing naturally in the system. Concentration-related responses of macrophytes and algae were obtained for the endpoints selected, resulting in a no observed ecologically adverse effect concentration (NOEAEC) at simazine concentrations of 0.05 mg active ingredient/L after 84 days. E. canadensis was the most negatively affected species based on length increase, which was consistently a very sensitive parameter for all macrophytes. The experimental design presented might constitute a suitable alternative to conventional laboratory single-species testing. - Simazine at concentrations of 0.05 mg/L does not cause long-term negative effects to aquatic macrophytes or algae.
[en] Quantum mechanics is a physical theory for objects of the microcosm, e.g. for atoms or electrons. It has proven itself so far, but leads to the fact that we have to grant properties and relations, which are neither compatible with our common sense nor with the concepts of classical physics, to these objects. These peculiarities are presented and their meaning for our cognitive ability and for a world view is discussed.
[de]Die Quantenmechanik ist eine physikalische Theorie für Objekte des Mikrokosmos, also z.B. für Atome oder Elektronen. Sie hat sich bisher bestens bewährt, führt aber dazu, dass wir diesen Objekten Eigenschaften und Relationen zubilligen müssen, die weder mit unserem gesunden Menschenverstand noch mit den Begriffen der klassischen Physik vereinbar sind. Diese Merkwürdigkeiten werden vorgestellt und ihre Bedeutung für unser Erkenntnisvermögen und für ein Weltbild wird diskutiert.
[en] With a conjugative plasmid pJP4 carrying strain as the donor, two bioaugmentation experiments were conducted in a microcosm biofilm reactor with 2,4-D as the sole carbon source operated in fed-batch mode, and an enlarged lab-scale sequence batch biofilm reactor with mixed carbon sources of 2,4-D and other easily biodegradable compounds, respectively. In the microcosm study under sole carbon source condition, bioaugmentation led to a persistently increased 2,4-D degradation rate in the five operation cycles with enhancement of 13-64%. For the enlarged lab-scale bioaugmentation experiment under mixed carbon source conditions, no enhancement in 2,4-D removal could be observed during start-up period. After a period of operation, biofilm samples from the bioaugmented reactor demonstrated a stronger degradation capacity than the control and showed the presence of a large number of transconjugants. This study indicates that bioaugmentation based on plasmid horizontal transfer is a feasible strategy to establish functional microbial community in a biofilm reactor, and the strong selective pressure of 2,4-D existing alone and persistently was more favorable for the success of gene augmentation.
[en] Microcosms are, literally, small worlds, and there are very nearly as many interpretations of what that means as there are researchers willing to use them. There is a lively debate on what constitutes a microcosm, and if there are any advantages to be gained over single-species ecotoxicological investigations (Cairns, 1983). As a result, few microcosm toxicity tests have any form of official sanction (van Gestel and van Straalen, 1994), and no soil microcosm tests are in widespread, routine usage. The American Society for Testing and Materials (ASTM) does list one soil microcosm methodology (E 1197-87, ASTM, 1987). As Golley (1985) comments, there are 4 or 5 million species of organisms on earth, and they each live in communities of 1000 to 2000 species with substantial ecological interconnections. We cannot build systems as complex, and yet we must study impacts on such systems. Microcosms offer an additional step on the way from studies of single species toward studies of true ecological complexity. (author)
[en] A semicontinuous slurry-microcosm method was applied to mimic trichloroethylene (TCE) cometabolic biodegradation field results at the Que-Jen in-situ pilot study. The microcosm study confirmed the process of aerobic cometabolism of TCE using toluene as the primary substrate. Based on the nucleotide sequence of 16S rRNA genes, the toluene-oxidizing bacteria in microcosms were identified, i.e. Ralstonia sp. P-10 and Pseudomonasputida. The first-order constant of TCE-degradation rate was 0.5 day-1 for both Ralstonia sp. P-10 and P.putida. The TCE cometabolic-biodegradation efficiency measured from the slurry microcosms was 46%, which appeared pessimistic compared to over 90% observed from the in-situ pilot study. The difference in the TCE cometabolic-biodegradation efficiency was likely due to the reactor configurations and the effective time duration of toluene presence in laboratory microcosms (1 days) versus in-situ pilot study (3 days). The results of microcosm experiments using different toluene-injection schedules supported the hypothesis. With a given amount of toluene injection, it is recommended to maximize the effective time duration of toluene presence in reactor design for TCE cometabolic degradation
[en] Microbial assisted phytoremediation is a promising, though yet poorly explored, new remediation technique. The aim of this study was to develop autochthonous microbial consortia resistant to cadmium that could enhance phytoremediation of salt-marsh sediments contaminated with this metal. The microbial consortia were selectively enriched from rhizosediments colonized by Juncus maritimus and Phragmites australis. The obtained consortia presented similar microbial abundance but a fairly different community structure, showing that the microbial community was a function of the sediment from which the consortia were enriched. The effect of the bioaugmentation with the developed consortia on cadmium uptake, and the microbial community structure associated to the different sediments were assessed using a microcosm experiment. Our results showed that the addition of the cadmium resistant microbial consortia increased J. maritimus metal phytostabilization capacity. On the other hand, in P. australis, microbial consortia amendment promoted metal phytoextraction. The addition of the consortia did not alter the bacterial structure present in the sediments at the end of the experiments. This study provides new evidences that the development of autochthonous microbial consortia for enhanced phytoremediation of salt-marsh sediments contaminated with cadmium might be a simple, efficient, and environmental friendly remediation procedure. Capsule abstract: Development of autochthonous microbial consortia resistant to cadmium that enhanced phytoremediation by salt-marsh plants, without a long term effect on sediment bacterial diversity. - Highlights: • Cd resistant microbial consortia were developed and applied to salt-marsh sediments. • In Phragmites australis the consortia amendment promoted metal phytoextraction. • The consortia addition increased Juncus maritimus phytostabilization capacity. • No long term changes on the rhizosediment bacterial structure were observed
[en] Polychlorinated biphenyl (PCB) congeners (PCB 52, 77, and 153) singly and in mixture were spiked and aged in soil microcosms and subsequently planted with switchgrass (Panicum virgatum) or poplar (Populus deltoids x nigra DN34). The planted reactors showed significantly greater reductions in PCB parent compounds when compared to unplanted systems after 32 weeks. There was evidence of reductive dechlorination in both planted and unplanted systems, but the planted microcosms with fully developed roots and rhizospheres showed greater biotransformation than the unplanted reactors. These dechlorination products accounted for approximately all of the molar mass of parent compound lost. Based on the transformation products, reductive dechlorination pathways are proposed for rhizospheric biotransformation of PCB 52, 77, and 153. This is the first report of rhizosphere biotransformation pathways for reductive dechlorination in marginally aerobic, intermittently flooded soil as evidenced by a mass balance on transformation products. -- Highlights: •Soil was spiked and aged and then planted with poplar and switchgrass. •Planted microcosms showed significant reductive dechlorination and greater biotransformation than unplanted reactor. •Rhizospheric reductive dechlorination pathways are proposed. -- This study provides insight into rhizospheric transformation of PCBs
[en] The efficiency of biopurification systems to treat pesticide-contaminated water was previously studied in microcosms. To validate the obtained results, macrocosm systems were set-up. Four pesticides (linuron, isoproturon, bentazone, and metalaxyl) were continuously applied to ten different organic substrate mixes. Retention of the pesticides was similar and in some cases slightly lower in the macrocosms compared to the microcosms. Differences in retention between the different mixes were however minimal. Moreover, the classification of the retention strength of the pesticides was identical to that observed in microcosms: linuron > isoproturon > metalaxyl > bentazone. Monod kinetics were used to describe delayed degradation, which occurred for isoproturon, metalaxyl and bentazone. No breakthrough of linuron was observed, thus, this pesticide was appointed as the most retained and/or degraded pesticide, followed by isoproturon, metalaxyl and bentazone. Finally, most of the matrix mixes efficient in degrading or retaining pesticides were mixes containing dried cow manure. - Transport of pesticides in macrocosm containing organic substrates
[en] The efficiency of a biopurification system, developed to treat pesticide contaminated water, is to a large extent determined by the chemical and hydraulic load. Insight into the behaviour of pesticides under different fluxes is necessary. The behaviour of metalaxyl, bentazone, linuron, isoproturon and metamitron was studied under three different fluxes with or without the presence of pesticide-primed soil in column experiments. Due to the time-dependent sorption process, retention of the pesticides with intermediate mobility was significantly influenced by the flux. The higher the flux, the slower pesticides will be sorbed, which resulted in a lower retention. Degradation of the intermediate mobile pesticides was also submissive to variations in flux. An increase in flux, led to a decrease in retention, which in turn decreased the opportunity time for biodegradation. Finally, the presence of pesticide-primed soil was only beneficial for the degradation of metalaxyl. - Retention and degradation of pesticides in microcosms liable to different fluxes.