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[en] This work explore the use of supercritical CO2 drying as alternative technique for the obtainment of pasteurized and high quality dried product. Several tests were conducted on animal, vegetable and fruit matrixes in order to investigate the effectiveness of SC-CO2 drying process at different process conditions. Design of experiment was performed to find the optimal process conditions for vegetable and fruit matrices, using the final water activity of the products as key indicator for the drying efficiency. The inactivation of naturally present microorganisms and inoculated pathogens demonstrated the capability of SC- CO2 drying process to assure a safe product. Moreover, retention of nutrients was compared with conventional drying methods. Results suggest that supercritical drying is a promising alternative technology for food drying. (Author)
[en] This project began with the simple goal of trying to understand the diversity of dissimilatory metal-reducing microorganisms that might be found in subsurface environments. It ended with a sophisticated understanding not only of what microorganisms are important for metal reduction in uranium-contaminated subsurface environments, but also their physiological status during in situ uranium bioremediation. These findings have provided unprecedented insight into uranium bioremediation and the methods by which this process might be optimized. A brief summary of the major accomplishments of the project is given.
[en] Irradiation of sewage sludge is a very important process to eliminate all types of microorganisms and to improve its characteristics, after the irradiation process sewage sludge will have a very good ecological, economic and agricultural value where it can be used safely as a fertilizer because of its nutrient value. Sewage samples were brought from Alhadba Alkhadra wastewater treatment plant in Tripoli and the following microorganisms were isolated: E.coli, Klebsiella, Campylobacter jejune, Bacillus sp., Yeast, Fungi. Samples were then divided into seven groups and received different doses ranged between 0.5 and 3 kGy with an increment of 0.5, one sample was kept as a control sample. The effect of the ionizing radiation was noticed as yeast and fungi disappeared after receiving a dose of 0.5 kGy where as E.Coli and Campylobacter jejune needed a dose of 1 kGy to be eliminated from sludge, a dose of 1.5 kGy was enough for Klebsiella pneumonia, Finally a dose of 3 kGy was needed to clear off the Bacillus sp. bacteria. Ph and E.C. were recorded before and after the irradiation process with no changes.
[en] Biotechnology industry is now a global 'Mega-Trend' and metabolic engineering technology has important role is this area. Therefore, many countries has made efforts in this field to produce top value added bio-products efficiently using microorganisms. It has been applied to increase the production of chemicals that are already produced by the host organism, to produce desired chemical substances from less expensive feedstock, and to generate products that are new to the host organism. Recent experimental advances, the so-called '-omics' technologies, mainly functional genomics, proteomics and metabolomics, have enabled wholesale generation of new genomic, transcriptomic, proteomic, and metabolomic data. This report provides the insights of the integrated view of metabolism generated by metabolic engineering for biotechnological applications of microbial metabolic engineering
[en] Alginate microcapsules containing cell yeasts of the species Saccharomyces cerivisae, used as a reference microorganism, were studied here to improve the protection of cell activity during food processing. Here a novel drying process was proposed to optimize processing conditions. The dehydration of microcapsules by microwaves and under near fluidizing conditions (NFMD), allows performing dehydration employing lower temperatures to maintain high viability levels and a high quality end product. Thus, strategies based on the combination of different thermal gradients and processing temperatures were analysed through a series of NFMD experiments. (Author)
[en] A method for identifying microorganisms is claimed. An emissive agent is added to a specimen of microorganisms to produce a mix of emissive products. These products are detected and characteristic pattern functioning as an identifier for the microorganisms is derived. The identifier is then compared with identifiers representing known microorganisms
[en] The radiosensitivity of 520 microbial strains isolated from the antibiotic preparations Levorin, Mycoheptane, and amphotericin B was studied. The D10 did not exceed 90 Krads for 85 percent of the strains. The most resistant were Bac. subtilis spores, their D10 being 140-200 Krads. (author)