[en] Highlights: • MiR-139-5p suppresses the migration and invasion of osteosarcoma cell lines. • MiR-139-5p decreases DNMT1 expressions in osteosarcoma lines. • MiR-139-5p reduces the osteosarcoma cell migration and invasion by regulating DNMT1 and suppresses osteosarcoma tumor growth in vivo. Accumulating evidence has suggested the crucial roles of differentially expressed miRNAs in osteosarcoma progression. MiR-139-5p was decreased in various cancers. However, the role of miR-139-5p in the development of osteosarcoma and the underlying mechanism remain to be addressed.

[en] Published in summary form only

[en] We report an osteoblastoma-like osteosarcoma in the right proximal fibula in a 22-year-old woman. Radiographs showed an irregular osteolytic lesion from the metaphysis to the epiphysis in the proximal fibula with partial destruction of cortical bone. Tissue from a biopsy indicated a typical osteoblastoma. Curettage and bone graft was performed. One year after the surgery, local recurrence occurred, and a wide excision was performed. Histological examination of the en-bloc surgical specimen revealed the tumor had permeated through the host bony trabeculae, although the nuclear atypia was not marked. Immunohistochemical expression of MIB-1 was detected in 9.0% of cells. (orig.)

[en] Two major questions are considered important in the evaluation of skeletal metastasis, namely: how to reduce the false-negative and false-positive results and, how to choose the indications for bone scintigraphy to achieve maximum yield. In this paper these questions are discussed according to our experience and an overview of the recent literature is given. In order to get a high yield of positive results, the indications for bone scintigraphy in the evaluation of malignant bone disease are: 1. a clinical suspicion of skeletal metastases, 2. breast cancer stage 3 and 4, 3. primary bone tumours in children, 4. prostatic cancer and 5. oatcell carcinoma. (orig.)

[en] Alveolar soft part sarcoma (ASPS) is a rare malignant soft tissue tumor, which rarely occurs in bone. We present a case of ASPS in a 23-year-old man with a 2-month history of back pain. Computed tomography scanning and magnetic resonance images demonstrated a destructive process in the 12th thoracic vertebra associated with a unilateral soft tissue mass. The tumor showed evidence of hypervascularity on MRI; it obviously was enhanced on T1-weighted images after injection of Gd-GDPA, and signal voids were shown on all pulse sequences which may help to differentiate ASPS from other tumors of the vertebra. We believe that this is the first case of ASPS arising in a vertebra. (orig.)

[en] Highlights: • LncRNA LINC00858 was significantly upregulated in both osteosarcoma tissues and cell lines. • LINC00858 silencing repressed osteosarcoma cells’ proliferation and invasion, and inhibited the tumor growth. • LINC00858 was identified to sponge miR-139 to form RISC using luciferase reporter and RIP. • LINC00858/miR-139/CDK14 axis promotes the tumorigenesis of osteosarcoma. Long noncoding RNAs (lncRNAs) have been identified to modulate the tumorigenesis of human cancers. The in-depth of lncRNAs on human osteosarcoma oncogenesis is still ambiguous. In present study, functional and mechanism experiments were conducted to investigate the role of long intergenic non-protein coding RNA 00858 (LINC00858) on human osteosarcoma tumorigenesis. Results demonstrated that LINC00858 expression was significantly upregulated in both osteosarcoma tissues and cell lines. Mechanism assays presented that LINC00858 silencing significantly repressed osteosarcoma cells' proliferation and invasion in vitro, and inhibited the tumor growth in vivo. In further experiments, LINC00858 was identified to sponge miR-139 to form RNA-induced silencing complex (RISC) using luciferase reporter assay and RNA immunoprecipitation (RIP). Besides, CDK14 was validated to be the target protein the miR-139. Rescue experiments confirmed the role of LINC00858/miR-139/CDK14 pathway on osteosarcoma cells' phenotype. In summary, these data prove that LINC00858/miR-139/CDK14 axis promotes the tumorigenesis of osteosarcoma, providing a new mechanism or target for osteosarcoma.

[en] Highlights: • FOXD1 is overexpressed in osteosarcoma while MiR-30a-5p is lowexpressed. • MiR-30a-5p directly regulates FOXD1 by binding its 3′-UTR. • Inhibition of FOXD1 suppresses osteosarcoma cell migration and invasion. • Inhibition of FOXD1 suppresses osteosarcoma cell proliferation in vitro and in vivo. Despite a number of studies have emphasized the extensive role of microRNA (miRNA) in the development of multiple cancers, the role of miR-30a-5p in the progression of osteosarcoma (OS) and the underlying mechanism are still limited. We detected the expression level of MiR-30a-5p and forkhead box D1 (FOXD1) in Clinical OS specimens and found that miR-30a-5p was significantly decreased while FOXD1 was markedly increased. Dual luciferase assay confirmed that FOXD1 was directly regulated by miR-30a-5p. In vitro assay showed that inhibitior of FOXD1 suppressed cell proliferation, migration and invasion in MG63 and U2OS cells, while overexpression of FOXD1 promoted OS cell proliferation and migration. In vivo assay further showed the inhibition of tumor growth after knockdown of FOXD1. These results suggested that FOXD1 might play key roles in OS development and progression, and was negatively regulated by miR-30a-5p in OS.

[en] Highlights: • Tumor thrombus is frequently seen in the large veins draining primary pelvic osteosarcoma on cross sectional imaging studies. • Accurate detection of tumor thrombus in the large draining veins on the pre-operative imaging may impact surgical margins. • In surgically resected cohort, patients with tumor thrombus showed statistically significant lower overall survival. - Abstract: PurposeTo evaluate the frequency of tumor thrombus in the large veins draining primary pelvic osteosarcoma on early cross-sectional imaging studies and its effect on patient survival.

[en] Highlights: • miR-15b-5p inhibits proliferation of OS cells. • miR-15b-5p reverse the Warburg effect in OS cells. • PDK4 is a direct target of miR-15b-5p. • PDK4 promotes the proliferation of OS cells and contributes to the Warburg effect in OS cells. • miR-15b-5p exerted anti-cancer effects on OS via inhibiting the expression of PDK4. Blocking aerobic glycolysis has been proposed as an attractive therapeutic strategy for impairing the proliferation of cancer cells. However, the underlying mechanisms are poorly understood. Here, we show that miR-15b-5p was downregulated in osteosarcoma (OS) and that lower expression of miR-15b-5p promoted proliferation and contributed to the Warburg effect in OS cells. Mechanistically, miR-15b-5p acted as a tumor suppressor in OS by directly targeting pyruvate dehydrogenase kinase-4 and inhibiting its expression. These results reveal a previously unknown function of miR-15b-5p in OS, which is associated with metabolic alterations that promote cancer progression. miR-15b-5p may play an essential role in the molecular therapy of patients with OS.

[en] 135 patients with histologically proven Ewing's sarcoma, treated at the Institute Gustave Roussy between 1960-1974 were reviewed in order to establish the effectiveness and the complications associated with radiotherapy. In 133 patients, a dose of at least 60 Gy for long bones, 55 Gy for flat bones and 45 Gy for vertebrae was given. 26 major complications (of which more than one occured in certain patients) were observed: 12 cases of growth retardation, loss of joint function in 7 cases, 3 fractures, 2 osteonecroses, 2 cases of delayed healing, one massive soft tissue fibrosis, 1 abdominocutaneous fistula, 2 cases of symptomatic pulmonary fibrosis with associated infection, 2 cases of radiation enteropathy, 2 of mammary hypoplasia and 1 osteosarcoma developing in an irradiated zone. These complications necessitated 12 surgical interventions, including 4 disarticulations or amputations. The incidence of complications can be related to the age of the patient and to irradiation dose