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[en] The improvement of ecological and technological qualities of Maritza East coals after biodesulfurization with Pseudomonas is reported. It is underlined that after laboratory treatment of coals with species of Pseudomonas some decrease of total sulfur is found out if the treatment is done at 300oC= 6,8 – 6,9 and in media Reimond
[en] Pseudomonas aeruginosa is an improtant opportunistic pathogen that can cause infection in immunocompromised patient. Lipopolysaccharide (LPS), the major surface antigen of P. aeruginosa, is immunogenic and elieits protective antibodies in animals. The O-polysaccharids (O-PS) from international Antigenic typing Scheme (IATS) 10, the antigenic determinant of LPS, was coupled to recombinant exoprotein A (rPA) through adipic acid dihydrazide (ADH) mediated by carbodiimide condensation reaction. Mice were immunized with the conjugate emulsifield with monophosphoryl lipid A-trehalose dicorynomycolate (MPL-T) and freund's adjuvants. The conjiugate emulsified with MPL-T adjuvant elicited the highest level of IgG and IgM followed by freuns's adjuvant. IgG titers using both MPL-T and freund's adjuvants were recorded to be higher than IgM titers after the second post of the immunization. Immunization of mice with the prepared conjugates emulsified with MPL-T and freund's adjvaided provide high level of protection (100%) against ten times the LD50 of homologous strain of P. aeruginsoa. the elicited high IgG level and the in vivo protection test results provided good evidences for the possible protection of the conjugate aginst subsequent infection with the pathogen. These findings will enable us to use it as protective vaccine candidate (authors).
[en] Polyhydroxyalkanoates (PHAs) have been produced by various bacteria as natural polymers stored in bacterial cells as a source of carbon and energy. They are currently preferred biomaterials for use in many industrial fields instead of conventional non-degradable plastics. Due to their unique properties they can reduce pollution caused by the increasing global polymer demand. Pseudomonas species have been chosen as PHAs producers in many recent studies. Being metabolically versatile and possessing a remarkable tolerance to a wide range of carbon sources, these bacteria have become an efficient cell factory for PHAs production. Currently, attention is focused on the design of Pseudomonas strains to increase their ability to accumulate PHAs in the cell and modifying their biosynthetic pathways to obtain strains with modified compositions and improved properties. This article discusses the current state of knowledge of polyhydroxyalkanoates synthesized by Pseudomonas species which are industrially important microorganisms. This review provides an overview of recent trends towards PHA production, focusing on the utilization of low-cost carbon sources, fermentation strategies, PHAs properties and their uses as valuable bioproducts.
[en] The bacterial community of an anaerobic granular sludge associated with uranium depletion was investigated following its exposure to uranium under different initial pH conditions (pH 4.5, 5.5, and 6.5). The highest uranium removal efficiency (98.1%) was obtained for the sample with an initial pH of 6.5, which also supported the highest bacterial community richness and diversity. Venn diagrams visualized the decrease in the number of genera present in both the inoculum and the uranium-exposed biomass as the initial pH decreased from 6.5 to 4.5. Compared with the inoculum, a significant increase in the abundances of the phyla Chloroflexi and Proteobacteria was observed following uranium exposure. At initial pH conditions of 6.5 to 4.5, the proportions of the taxa Anaerolineaceae, Chryseobacterium, Acinetobacter, Pseudomonas, and Sulfurovum increased significantly, likely contributing to the observed uranium removal. Uranium exposure induced a greater level of dynamic diversification of bacterial abundances than did the initial pH difference.
[en] The results of examination of 143 children with acute purulent destructive pneumonia caused by Gram-negative microorganisms (in a monoculture or in association with staphylococcus) have been analyzed. The X-ray appearance of acute purulent destructive pneumonia caused by Pseudomonas aeruginosa is presented. The importance of X-ray examination for the early diagnosis of acute purulent destructive pneumonias is emphasized: a conjecture can thus be made on the etiology of the disease and specific treatment initiated
[en] As important human and veterinary medicines, antibiotics are being produced and consumed in large quantities around the world. For example, more than 50 million pounds (22,000 tons) of antibiotics are produced in the U.S. each year and annual production in Germany is about 2,000 tons. Antibiotics are low molecular weight microbial metabolites that at low concentrations inhibit the growth of other microorganisms. Resistant bacteria may also spread and become broader infection-control problems, not only within health care institutions, but in communities as well. Clinically important bacteria, such as methicillin-resistant Staphylococcus aureus (MRSA). MRSA is a common cause of infection among hospitalized patients. Pseudomonas aeruginosa is a major cause of opportunistic infections among immunocompromised individuals. The spread of this organism in health care settings is often difficult to control due to the presence of multiple intrinsic and acquired mechanisms of antimicrobial resistance. In this study, we isolated novel bacterium which had strong antagonistic activity and separated antibiotic compounds from Pseudomonas sp., and analyzed characteristics and molecular weight of the antibiotic compound
[en] In the joint chemical reduction of silver and copper ions in the presence of gelatin, regardless of the composition of the reaction medium, a nanocomposite was formed consisting of nanodispersed silver and copper particles. In this research, the dependence of phase composition and dispersity on the synthesis conditions, as well as antibacterial activity of synthesized silver and copper nanocomposites were studied. It has been established that the most highly dispersed silver and copper nanocomposites were formed in an alkaline medium. We have revealed that nanocomposites of Ag-Cu system which were synthesized in an alkaline medium possessed significant antibacterial activity against the Erwinia amylovora and Pseudomonas syringae bacteria. (paper)
[en] The objective of this study was to observe the change in frequency of resistance gene (Rpg4) to Pseudomonas syringae pv. Glycinae (Psg) between the ancestors and public soybean cultivars. A comparison of the alleles at the locus for resistance of soybean (Glycine max, L.) to bacterial blight in a sample of 51 North American public cultivars released through 1990 with the allele in their ancestors indicated that breeders have concentrated the dominant allele in public cultivars from 69 to 84%. The Rpg4 gene may be involved in resistance to Psg in soybean, however, it has another useful function that somehow contributes to soybean productivity in modern agroecosystems and thus plant breeders have unintentionally increased its frequency in cultivated germplasm.(author)