[en] In cancer cells, anticancer reagents often trigger nuclear accumulation of YB-1, which participates in the progression of cancer malignancy. YB-1 has a non-canonical nuclear localization signal (YB-NLS). Here we found that four nucleocytoplasmic-shuttling RNA-binding proteins and p53 interact specifically with the YB-NLS and co-accumulate with YB-1 in the nucleus of actinomycin D-treated cells. To elucidate the roles of these YB-NLS-binding proteins, we performed a dominant-negative experiment in which a large excess of YB-NLS interacts with the YB-NLS-binding proteins, and showed inhibitory effects on actinomycin D-induced nuclear transport of endogenous YB-1 and subsequent MDR1 gene expression. Furthermore, the YB-NLS-expressing cells were also found to show increased drug sensitivity. Our results suggest that these YB-NLS-associating proteins are key factors for nuclear translocation/accumulation of YB-1 in cancer cells. - Highlights: • Four nucleocytoplasmic-shuttling proteins and p53 associate with YB-NLS. • They showed nuclear co-accumulation with YB-1 in actinomycin D-treated cells. • Overexpression of YB-NLS was carried out to take YB-NLS-binding proteins from YB-1. • YB-NLS inhibited actinomycin D-induced nuclear localization of endogenous YB-1. • YB-NLS suppressed actinomycin D-induced expression of MDR1.

[en] Highlights: • A HRELP fusion tag is designed and highly expressed in E. coli. • HRELP fusion tag can be specifically and quickly stained with Pauly's reagent. • HRELP fusion protein can be detected in both cell lysate and SDS-PAGE. • HRELP fusion protein can be purified by pH-triggered ITC. Although many protein fusion tags have been developed for recombinant protein production to improve protein yields or facilitate purification, determining the expression and purification of the fusion protein still remain to be a time-consuming and laborious procedure. In this work, we designed a histidine-rich elastin-like polypeptide (HRELP) fusion tag and found that it could be efficiently expressed in E. coli cells and specifically stained with Pauly's reagent in a couple of minutes post SDS-PAGE analysis. Moreover, in Pauly's reagent-stained polyacrylamide gels, only the bands of HRELP fusion proteins were yellow and could be clearly visualized with little background. Furthermore, both HRELPs and HRELP20-BMP2 fusion protein could be purified by a method of pH shift-mediated inverse transition cycling (ITC). In our opinion, the HRELP established in this study may be considered as a multifunctional protein tag which could make its fusion proteins being quickly detected by Pauly staining and simply purified by pH-triggered ITC in addition to having the potential to sustained release its fusion proteins.

[en] The results of this study suggest that the single reagent method is suitable for application to the double antibody serum thyroxine radioimmunoassay, with a considerable saving in operator time and no loss in assay performance. A comparison of 66 clinical samples analysed by this and multiple reagent methods has been made. The correlation coefficient was 0.996. (author)

[en] The invention concerns a new radio-immunological method for determining a thyroid hormone such as thyroxine or triiodothyronine in an aqueous sample, characterised in that it consists in bringing into contact with hydrolised particles of cross-linked polyacrylamide of a diameter in around the range 0.1 and 10 microns or in the non hydrolised form, in covalent liaison with antibodies relative to the thyroid hormone to be determined, the aqueous sample containing the hormone and this hormone labelled with a radioactive isotope to fix a part of the labelled hormone and the unlabelled hormone to this antibody so as to produce a two phase system comprising a solid phase containing the tied part of the labelled hormone and the unlabelled hormone and a liquid phase containing the non tied part of the labelled hormone and the unlabelled hormone; in separating the two phases from each other and in measuring the radioactivity of at least one of the solid and liquid phases, the value of the radioactivity depending on the concentration of the hormone in the aqueous sample

[en] Full text: The structure and properties of pyrohallole based monoazocompounds by elemental analysis, 1H-NMR, IR, UV and roentgen electronic spectroscopy methods have been studied. Spectra chemical criteria of taut metric transformations have been established on the instance of TSNAB. There have been determined azo-and amino hydro zone forms of crystalline states of the reagents

[en] This review is intended to generalize and systematize available information on the synthesis of imidazo[1,5-a]- and imidazo[1,2-a]quinoxalines. Relevant studies published in the last 10–15 years, as well as earlier studies less familiar to the broad chemical community, are surveyed. Major methods of formation of the imidazoquinoxaline system from substituted quinoxalines and imidazoles according to the type of bond being formed are presented. Special focus is placed on the radically new methods that enable the synthesis of various derivatives of these heterocyclic systems from available and inexpensive reagents. The mechanisms of formation of these compounds are discussed. Information on the biological activity of imidazo[1,5-a]- and imidazo[1,2-a]quinoxalines and examples of pharmaceuticals based on them are reported. The bibliography includes 179 references

No abstract available

[en] Surface-modified polymers are of substantial importance in many diverse aspects of modern technology, and whilst there are a number of existing physical and chemical methods for surface modification of polymers, the frequent requirement for significant infrastructure, harsh reaction conditions and limitation to specific polymer types led us to consider alternative chemical methods. A desirable alternative would be that amenable to a large range of polymers, permitting direct chemical modification under mild conditions and using inexpensive reagents. We report here that functionalized diarylcarbenes are excellent reactive intermediates suitable for direct surface modification of a range of organic and inorganic materials, and we have illustrated that this can be used for the introduction of visible and fluorescent chromophores, biocidal and biocompatible function

[en] Data on chemical compounds capable of cleaving ribonucleic acids under physiological conditions are systematised and generalised. The possibilities of the design of site-specific RNA-cleaving reagents are considered. The bibliography includes 245 references.

[en] The aim of this this paper was to investigate the effectiveness of flotation process depending on the changeable dose of reagents and variable speed of rotor in flotation device. HPGR crushing products were used as a feed in all flotation tests. Efficiency indices, along with Fuerstenau curves, were determined for each test. Both rotational speed and the dose of reagent have affected the flotation results in a lower degree that expected. However it could be possible to determine the optimal dose and rotational speed of rotor that increase the effectiveness of flotation process. (paper)