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AbstractAbstract
[en] In cancer cells, anticancer reagents often trigger nuclear accumulation of YB-1, which participates in the progression of cancer malignancy. YB-1 has a non-canonical nuclear localization signal (YB-NLS). Here we found that four nucleocytoplasmic-shuttling RNA-binding proteins and p53 interact specifically with the YB-NLS and co-accumulate with YB-1 in the nucleus of actinomycin D-treated cells. To elucidate the roles of these YB-NLS-binding proteins, we performed a dominant-negative experiment in which a large excess of YB-NLS interacts with the YB-NLS-binding proteins, and showed inhibitory effects on actinomycin D-induced nuclear transport of endogenous YB-1 and subsequent MDR1 gene expression. Furthermore, the YB-NLS-expressing cells were also found to show increased drug sensitivity. Our results suggest that these YB-NLS-associating proteins are key factors for nuclear translocation/accumulation of YB-1 in cancer cells. - Highlights: • Four nucleocytoplasmic-shuttling proteins and p53 associate with YB-NLS. • They showed nuclear co-accumulation with YB-1 in actinomycin D-treated cells. • Overexpression of YB-NLS was carried out to take YB-NLS-binding proteins from YB-1. • YB-NLS inhibited actinomycin D-induced nuclear localization of endogenous YB-1. • YB-NLS suppressed actinomycin D-induced expression of MDR1.
Primary Subject
Source
S0006-291X(16)31393-6; Available from http://dx.doi.org/10.1016/j.bbrc.2016.08.129; Copyright (c) 2016 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 478(3); p. 1363-1369

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AbstractAbstract
[en] Highlights: • A HRELP fusion tag is designed and highly expressed in E. coli. • HRELP fusion tag can be specifically and quickly stained with Pauly's reagent. • HRELP fusion protein can be detected in both cell lysate and SDS-PAGE. • HRELP fusion protein can be purified by pH-triggered ITC. Although many protein fusion tags have been developed for recombinant protein production to improve protein yields or facilitate purification, determining the expression and purification of the fusion protein still remain to be a time-consuming and laborious procedure. In this work, we designed a histidine-rich elastin-like polypeptide (HRELP) fusion tag and found that it could be efficiently expressed in E. coli cells and specifically stained with Pauly's reagent in a couple of minutes post SDS-PAGE analysis. Moreover, in Pauly's reagent-stained polyacrylamide gels, only the bands of HRELP fusion proteins were yellow and could be clearly visualized with little background. Furthermore, both HRELPs and HRELP20-BMP2 fusion protein could be purified by a method of pH shift-mediated inverse transition cycling (ITC). In our opinion, the HRELP established in this study may be considered as a multifunctional protein tag which could make its fusion proteins being quickly detected by Pauly staining and simply purified by pH-triggered ITC in addition to having the potential to sustained release its fusion proteins.
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Source
S0006291X18324446; Available from http://dx.doi.org/10.1016/j.bbrc.2018.11.038; Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 507(1-4); p. 343-347

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AbstractAbstract
[en] The results of this study suggest that the single reagent method is suitable for application to the double antibody serum thyroxine radioimmunoassay, with a considerable saving in operator time and no loss in assay performance. A comparison of 66 clinical samples analysed by this and multiple reagent methods has been made. The correlation coefficient was 0.996. (author)
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Record Type
Journal Article
Journal
Annals of Clinical Biochemistry; ISSN 0004-5632;
; v. 17(pt.6); p. 339-340

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Wegfahrt, P.F.; Lewin, Nathan; Monthony, J.F.
Bio-Rad Labs., Richmond, CA (USA)1976
Bio-Rad Labs., Richmond, CA (USA)1976
AbstractAbstract
[en] The invention concerns a new radio-immunological method for determining a thyroid hormone such as thyroxine or triiodothyronine in an aqueous sample, characterised in that it consists in bringing into contact with hydrolised particles of cross-linked polyacrylamide of a diameter in around the range 0.1 and 10 microns or in the non hydrolised form, in covalent liaison with antibodies relative to the thyroid hormone to be determined, the aqueous sample containing the hormone and this hormone labelled with a radioactive isotope to fix a part of the labelled hormone and the unlabelled hormone to this antibody so as to produce a two phase system comprising a solid phase containing the tied part of the labelled hormone and the unlabelled hormone and a liquid phase containing the non tied part of the labelled hormone and the unlabelled hormone; in separating the two phases from each other and in measuring the radioactivity of at least one of the solid and liquid phases, the value of the radioactivity depending on the concentration of the hormone in the aqueous sample
[fr]
L'invention concerne une nouvelle methode radio-immunologique de dosage d'une hormone thyroidienne telle que la thyroxine ou la triiodothyronine dans un echantillon aqueux, caracterise par le fait qu'elle consiste a faire entrer en contact avec des particules hydrolysees de polyacrylamine reticule de diametre compris entre environ 0,1 et 10 microns ou sous la forme non hydrolysee, en liaison par covalence avec des anticorps relatifs a l'hormone thyroidienne a doser, l'echantillon aqueux contenant l'hormone et cette hormone marquee avec un isotope radio-actif pour fixer une partie de l'hormone marquee et de l'hormone non marquee audit anticorps de maniere a produire un systeme a deux phases comprenant une phase solide qui contient la partie liee de l'hormone marquee et de l'hormone non marquee et une phase liquide contenant la partie non liee de l'hormone marquee et de l'hormone non marquee, a separer les deux phases l'une de l'autre et a mesurer la radio-activite d'au moins l'une des phases solide et liquide, la valeur de la radio-activite etant fonction de la concentration de l'hormone dans l'echantillon aqueuxOriginal Title
Nouvelle methode radio-immunologique de dosage de l'hormone thyroidienne et reactif utilise
Primary Subject
Source
3 Dec 1976; 15 p; FR PATENT DOCUMENT 2363109/A/; Available from Institut National de la Propriete Industrielle, Paris (France); Priority claim: 27 Aug 1976, US.
Record Type
Patent
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AbstractAbstract
[en] Full text: The structure and properties of pyrohallole based monoazocompounds by elemental analysis, 1H-NMR, IR, UV and roentgen electronic spectroscopy methods have been studied. Spectra chemical criteria of taut metric transformations have been established on the instance of TSNAB. There have been determined azo-and amino hydro zone forms of crystalline states of the reagents
Original Title
Issledovaniestroeniya i svoystv O', O-dioksiazosoedineniy na osnove piroqallola
Primary Subject
Source
Available in abstract form only, full text entered in this record; Translated from Russian
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Journal Article
Literature Type
Translation
Journal
Azerbajdzhanskij Khimicheskij Zhurnal; ISSN 0005-2531;
; v. 23(5); 6 p

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AbstractAbstract
[en] This review is intended to generalize and systematize available information on the synthesis of imidazo[1,5-a]- and imidazo[1,2-a]quinoxalines. Relevant studies published in the last 10–15 years, as well as earlier studies less familiar to the broad chemical community, are surveyed. Major methods of formation of the imidazoquinoxaline system from substituted quinoxalines and imidazoles according to the type of bond being formed are presented. Special focus is placed on the radically new methods that enable the synthesis of various derivatives of these heterocyclic systems from available and inexpensive reagents. The mechanisms of formation of these compounds are discussed. Information on the biological activity of imidazo[1,5-a]- and imidazo[1,2-a]quinoxalines and examples of pharmaceuticals based on them are reported. The bibliography includes 179 references
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Source
Available from http://dx.doi.org/10.1070/RC2014v083n09ABEH004424; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
Journal
Russian Chemical Reviews (Print); ISSN 0036-021X;
; v. 83(9); p. 820-847

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AbstractAbstract
No abstract available
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Source
2 refs. Short communication only.
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Journal Article
Journal
Zeitschrift fuer Analytische Chemie; v. 263(4); p. 334
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Moloney, Mark G, E-mail: mark.moloney@chem.ox.ac.uk2008
AbstractAbstract
[en] Surface-modified polymers are of substantial importance in many diverse aspects of modern technology, and whilst there are a number of existing physical and chemical methods for surface modification of polymers, the frequent requirement for significant infrastructure, harsh reaction conditions and limitation to specific polymer types led us to consider alternative chemical methods. A desirable alternative would be that amenable to a large range of polymers, permitting direct chemical modification under mild conditions and using inexpensive reagents. We report here that functionalized diarylcarbenes are excellent reactive intermediates suitable for direct surface modification of a range of organic and inorganic materials, and we have illustrated that this can be used for the introduction of visible and fluorescent chromophores, biocidal and biocompatible function
Primary Subject
Source
S0022-3727(08)70616-8; Available from http://dx.doi.org/10.1088/0022-3727/41/17/174006; Country of input: International Atomic Energy Agency (IAEA)
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Journal Article
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AbstractAbstract
[en] Data on chemical compounds capable of cleaving ribonucleic acids under physiological conditions are systematised and generalised. The possibilities of the design of site-specific RNA-cleaving reagents are considered. The bibliography includes 245 references.
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Source
Available from http://dx.doi.org/10.1070/RC2001v070n06ABEH000610; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Literature Type
Bibliography
Journal
Russian Chemical Reviews (Print); ISSN 0036-021X;
; v. 70(6); p. 491-508

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AbstractAbstract
[en] The aim of this this paper was to investigate the effectiveness of flotation process depending on the changeable dose of reagents and variable speed of rotor in flotation device. HPGR crushing products were used as a feed in all flotation tests. Efficiency indices, along with Fuerstenau curves, were determined for each test. Both rotational speed and the dose of reagent have affected the flotation results in a lower degree that expected. However it could be possible to determine the optimal dose and rotational speed of rotor that increase the effectiveness of flotation process. (paper)
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Secondary Subject
Source
Mineral Engineering Conference; Zawiercie (Poland); 26-29 Sep 2018; Available from http://dx.doi.org/10.1088/1757-899X/427/1/012018; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Literature Type
Conference
Journal
IOP Conference Series. Materials Science and Engineering (Online); ISSN 1757-899X;
; v. 427(1); [8 p.]

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