Filters
Results 1 - 10 of 55037
Results 1 - 10 of 55037.
Search took: 0.054 seconds
Sort by: date | relevance |
Lyashenko, Andrey V.; Belova, Oksana; Gabdulkhakov, Azat G.; Lashkov, Alexander A.; Lisov, Alexandr V.; Leontievsky, Alexey A.; Mikhailov, Al’bert M., E-mail: amm@ns.crys.ras.ru2011
AbstractAbstract
[en] The purification, crystallization and preliminary X-ray structure analysis of the laccase from G. lucidum are reported. The ligninolytic enzymes of the basidiomycetes play a key role in the global carbon cycle. A characteristic property of these enzymes is their broad substrate specificity, which has led to their use in various biotechnologies, thus stimulating research into the three-dimensional structures of ligninolytic enzymes. This paper presents the purification, crystallization and preliminary X-ray analysis of the laccase from the ligninolytic basidiomycete Ganoderma lucidum
Source
S1744309111021129; Available from http://dx.doi.org/10.1107/S1744309111021129; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3151130; PMCID: PMC3151130; PMID: 21821897; PUBLISHER-ID: fw5311; OAI: oai:pubmedcentral.nih.gov:3151130; Copyright (c) International Union of Crystallography 2011; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
External URLExternal URL
Inobe, Tomonao; Tsukamoto, Masayuki; Nozaki, Miyuki, E-mail: inobe@eng.u-toyama.ac.jp2018
AbstractAbstract
[en] Highlights: • We constructed a fusion protein comprising ubiquitin and Rpn4-derived unstructured region. • The fusion ubiquitin retained its function of polyubiquitination of target proteins. • The fusion ubiquitin induced more efficient proteasomal target degradation than wild-type ubiquitin. Methods to induce proteasomal degradation of unwanted proteins are valuable in biomedical studies and thus receive increasing attention. For efficient degradation, the proteasome requires both a ubiquitin tag, which delivers substrates to the proteasome, and an unstructured region, where the proteasome engages the substrate for unfolding and degradation. We fused two degron components into a single molecule to create a fusion protein comprising ubiquitin and Rpn4-derived unstructured region. We demonstrated that the fusion protein retained its function to polyubiquitinate target proteins, thereby inducing more efficient proteasomal target degradation than wild-type ubiquitin in vitro and in cells. These results provide novel strategies for robust degradation enhancement of polyubiquitinated proteins.
Primary Subject
Source
S0006291X18311458; Available from http://dx.doi.org/10.1016/j.bbrc.2018.05.088; Copyright (c) 2018 Elsevier Inc. All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Biochemical and Biophysical Research Communications; ISSN 0006-291X;
; CODEN BBRCA9; v. 501(4); p. 948-954

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
Xue Chaohua; Jia Shuntian; Zhang Jing; Ma Jianzhong, E-mail: xuech@zju.edu.cn2010
AbstractAbstract
[en] This review summarizes the key topics in the field of large-area fabrication of superhydrophobic surfaces, concentrating on substrates that have been used in commercial applications. Practical approaches to superhydrophobic surface construction and hydrophobization are discussed. Applications of superhydrophobic surfaces are described and future trends in superhydrophobic surfaces are predicted. (topical review)
Primary Subject
Source
Available from http://dx.doi.org/10.1088/1468-6996/11/3/033002; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Science and Technology of Advanced Materials; ISSN 1468-6996;
; v. 11(3); [15 p.]

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
BROWN, K.A.; CHIUEH, T.
Brookhaven National Lab., Upton, NY (United States). Funding organisation: BROOKHAVEN NATIONAL LABORATORY (United States)2000
Brookhaven National Lab., Upton, NY (United States). Funding organisation: BROOKHAVEN NATIONAL LABORATORY (United States)2000
AbstractAbstract
No abstract available
Primary Subject
Source
11 Sep 2000; 7 p; International computational accelerator physics conference ICAP 2000; Darmstadt (Germany); 10-14 Sep 2000; KA--040301; AC02-98CH10886; Also available from OSTI as DE00767189; PURL: https://www.osti.gov/servlets/purl/767189-WxePU7/native/
Record Type
Report
Literature Type
Conference
Report Number
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
Malek, C.K.
Proceedings of the 1. Workshop on Synchrotron Light: Applications and Related Instrumentation1988
Proceedings of the 1. Workshop on Synchrotron Light: Applications and Related Instrumentation1988
AbstractAbstract
[en] An overview of the various types of litography is presented and discussed (optical, X-rays, electron beam, ion beam etc.). (A.C.A.S.)
[pt]
Apresenta-se uma revisao e discute-se os varios tipos de litografia (otica, raios-X, feixes de eletrons, feixes de ions etc.). (A.C.A.S.)Primary Subject
Source
Laboratorio Nacional de Luz Sincrotron (LNLS), Campinas, SP (Brazil); 302 p; 1988; p. 214-235; 1. Workshop on Synchrotron Light: Applications and Related Instrumentation; Campinas, SP (Brazil); 25-28 Jul 1988; Available from the Library of the Comissao Nacional de Energia Nuclear, RJ, Brazil
Record Type
Miscellaneous
Literature Type
Conference
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] The highly conserved domain of unknown function in the cyanobactin superfamily has a novel fold. The protein does not appear to bind the most plausible substrates, leaving questions as to its role. Patellamides are members of the cyanobactin family of ribosomally synthesized and post-translationally modified cyclic peptide natural products, many of which, including some patellamides, are biologically active. A detailed mechanistic understanding of the biosynthetic pathway would enable the construction of a biotechnological ‘toolkit’ to make novel analogues of patellamides that are not found in nature. All but two of the protein domains involved in patellamide biosynthesis have been characterized. The two domains of unknown function (DUFs) are homologous to each other and are found at the C-termini of the multi-domain proteins PatA and PatG. The domain sequence is found in all cyanobactin-biosynthetic pathways characterized to date, implying a functional role in cyanobactin biosynthesis. Here, the crystal structure of the PatG DUF domain is reported and its binding interactions with plausible substrates are investigated
Source
Available from http://dx.doi.org/10.1107/S2053230X1402425X; Available from http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4259220; PMCID: PMC4259220; PMID: 25484206; PUBLISHER-ID: sx5117; PUBLISHER-ID: S2053230X1402425X; OAI: oai:pubmedcentral.nih.gov:4259220; Copyright (c) Mann et al. 2014; This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Acta crystallographica. Section F, Structural biology communications; ISSN 2053-230X;
; CODEN ACSFEN; v. 70(Pt 12); p. 1597-1603

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
External URLExternal URL
AbstractAbstract
[en] This article deals with the assembly of SMD chip components onto a flexible substrate by using non-conductive adhesives. In the experiments, two electrically conductive adhesives (ECA) and two non-conductive adhesives (NCA) were used. The verification of the properties and usability of NCAs for connecting the components to the flexible substrates as an alternative for ECA was the main goal of the experiment. The results show that NCAs can be used as an alternative for ECAs and that the properties and reliability of NCA joints are comparable or better than ECA joints. The results also show that UV-curable NCAs can be recommended for the applications of attaching the components on flexible substrates, especially for the prototypes, where the application of the UV adhesive and the connection of components by this technology is much easier and faster than with ECA.
Primary Subject
Secondary Subject
Source
Copyright (c) 2019 Springer Science+Business Media, LLC, part of Springer Nature; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Journal of Materials Science. Materials in Electronics; ISSN 0957-4522;
; CODEN JSMEEV; v. 30(15); p. 14214-14223

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
Asadi, Z. Soltani; Mahboubi, F., E-mail: z.soltaniasadi@gmail.com, E-mail: mahboubi@aut.ac.ir2012
AbstractAbstract
[en] Highlights: → The thickness of the compound layer increases with increasing in temperature and groove width. → Surface layer at the remote regions from the edge is thinner than that of closer regions. → The hardness and the case depth of the nitrided layer increase with increasing the width of the groove. → Intensity of ε phase increases with increasing the width of the groove in both methods. → The ASPN specimens are covered by hexagonal particles and for the CPN by cauliflower shape nitrides. -- Abstract: The main aim of this work was to investigate the effect of the sample geometry on properties of the conventional plasma nitrided (CPN) and active screen plasma nitrided (ASPN) steel. Sample assemblies consisting of rectangular grooved steel blocks with different groove dimensions of 2, 4, 6, 8 and 10 (W) x 40 (H) x 20 (L) mm3 and AISI 4340 steel plates (substrates) with dimensions of 10 x 40 x 60 mm3, to serve as groove cover, were prepared. The sample assemblies were conventional and active screen plasma nitrided under the gas mixture of 75%N2 + 25%H2, at temperatures of 500 oC and 540 oC, pressure of 4 torr, for 5 h. Properties of the nitrided substrates were investigated by evaluating compound layer thickness, case depth, phase composition and hardness profile. Results of the experiments showed that the thickness of the compound layer, hardness and nitrided case depth increased with increasing the width of the groove for both methods. Also, in each sample, nitrogen atoms penetrated more deeply in the regions of the groove closer to the edge. Hallow cathode effect occurred at the sample with 2 mm width groove, in the CPN method, leading to the overheating of the sample. In ASPN, the hardness and the nitrided case depth are lower in comparison with CPN. The surface morphology of the CPN treated samples consists of cauliflower shape surface nitrides while the surface of the AS plasma nitrided samples are covered by the hexagonal particles with uniform distribution.
Primary Subject
Secondary Subject
Source
S0261-3069(11)00336-0; Available from http://dx.doi.org/10.1016/j.matdes.2011.04.066; Copyright (c) 2011 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] Highlights: •IMC growth at the SAC/Cu–xZn solder interfaces strongly depends on the Zn concentration. •IMC morphology changed from scallop to layered type at the SAC/Cu–xZn–yNi interface. •Neither Cu3Sn nor microvoids was formed on both Cu–xZn and Cu–xZn–yNi substrates. •Zn-rich layer at the IMC/Cu–xZn or IMC/Cu–xZn–yNi was effective in suppressing the IMC growth. •IMC growth behavior is controlled by the Zn rather than Ni content in the Cu–xZn–yNi substrates. -- Abstract: The interfacial reactions of Sn–3.0Ag–0.5Cu (SAC 305) solder on Cu–xZn (x = 0 wt%, 10 wt%, 15 wt%, 30 wt%, and 35 wt%) substrates and Cu–xZn–yNi (x = 20 wt% and 25 wt%, y = 10 wt% and 15 wt%) were investigated after soldering and isothermal aging. SAC 305 solder was reacted with Cu–xZn and Cu–xZn–yNi substrates by dipping Cu–xZn or Cu–xZn–yNi wires into molten solder at 250 °C for 90 s. After soldering, Cu6Sn5-based intermetallic compound (IMC) formed at the solder interfaces. After aging at 150 °C, a bi-layer structure of Cu6Sn5/Cu3Sn formed, and microvoids were observed in the Cu3Sn layer at the SAC/Cu solder interfaces. For SAC/Cu–Zn samples, Cu6(Sn, Zn)5 was the predominant reaction product, and formation of Cu3Sn was not observed. Interfacial IMC growth was delayed as the Zn concentration increased. In addition, the Cu(Zn, Sn) phase formed at the Cu6(Sn, Zn)5/Cu–Zn interfaces in the Cu–xZn substrates with a high Zn concentration (30 wt% and 35 wt%). In the case of the Cu–xZn–yNi samples, the major interfacial IMC was (Cu, Ni)6(Sn, Zn)5 phase. The Ni atoms from the Cu–xZn–Ni substrates participated in the interfacial reaction at the SAC/Cu–xZn–yNi interfaces and resulting in the formation of a thick and layered IMC phase, comparing to those at the SAC/Cu–Zn interfaces. Although the effect of Ni addition to the Cu–Zn substrates exhibited delayed IMC growth, the Zn concentration in the Cu–xZn–yNi substrates influenced the IMC growth behavior more than the Ni concentration
Primary Subject
Source
S0925-8388(13)01327-3; Available from http://dx.doi.org/10.1016/j.jallcom.2013.05.167; Copyright (c) 2013 Elsevier Science B.V., Amsterdam, The Netherlands, All rights reserved.; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Journal
Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
AbstractAbstract
[en] The problem of determining the temperature of a substrate of dissimilar materials fragments in the process of infrared heating in vacuum solution, obtained using the algebraic method of calculating radiant fluxes, is presented. (paper)
Primary Subject
Secondary Subject
Source
26. International Conference on Vacuum Technique and Technology; Saint Petersburg (Russian Federation); 18-20 Jun 2019; Available from http://dx.doi.org/10.1088/1742-6596/1313/1/012009; Country of input: International Atomic Energy Agency (IAEA)
Record Type
Journal Article
Literature Type
Conference
Journal
Journal of Physics. Conference Series (Online); ISSN 1742-6596;
; v. 1313(1); [4 p.]

Country of publication
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue
1 | 2 | 3 | Next |