Results 1 - 10 of 1850
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[en] A sensitive double-antibody radioimmunoassay for staphylococcal enterotoxins A and B is described. The separation of the primary antigen-antibody complex of enterotoxin A and B was achieved with an anti-rabbit gamma globulin from goats. Radioiodinated aggregate fractions of staphylococcal enterotoxins exhibited reduced immunological activity and showed little competition with non-radioactive enterotoxin. The radioimmunoassay was successfully applied for the quantitation of enterotoxins in food. (author)
[en] The method is applicable to the determination of ochratoxin A in malting barley, malt and cereals in general; it can serve as an expeditious screening method. The principles, instrumentation and chemicals as well as the working procedure are described. The detection limit is 1.2 μg/kg, recovery (at 20 μg/kg) is 0.67 ± 0.08. (Z.S.). 1 ref
[en] In order to establish optimum conditions of radiosterilization, dry vaccines from anatoxics and typhoid antigen were irradiated on irradiation units of types EPGU-200 and LUE-25/8 using doses of 1.25, 2.5, and 10 Mrads. It was found that the dose of 2.5 Mrads assured complete sterility of the preparations including those contaminated with spores, without altering their biological properties. (author)
[en] The authors' findings indicate that irradiation confers no advantage over heat processing in respect of bacterial toxins (clostridium botulinum, neurotoxin A and staphylococcal enterotoxin A). It follows that irradiation at doses less than the ACINF recommended upper limit of 10 kGy could not be used to improve the ambient temperature shelf life on non-acid foods. (author)
[en] Okadaic acid (OKA), a marine toxin produced by dinoflagellates, is responsible for most human diarrhetic shellfish poisoning-associated health disorders. A competitive displacement assay for OKA is described here. An OKA-binding aptamer was truncated with two sequences, one labeled with 6-carboxyfluorescein (FAM), and one with a quencher. On addition of OKA, it will bind to the aptamer and green fluorescence pops up because label and quencher become spatially separated. One of the truncated aptamers exhibis an excellent binding capability (Kd 2.77 nM) for OKA compared to its full-length aptamer (526 nM). The selectivity of the assay was proven by the successful fluorometric determination of OKA in the presence of common diarrhoetic toxins and in shellfish extracts. The detection limit is as low as 39 pg·mL−1. .
[en] The purpose of this work is the development of sustainable colorimetric sensors for the detection of toxins in drinking water. These sensors are based on azulene and guaiazulene, two molecules with unique optical and chemical properties.
[en] Full text: The kinetics of degradation of natural polycyclic toxins in grains and dried fruits under the influence of UV-light and ionizing radiation 60C and the probability of radiolytic detoxication of these products has been studied for the first time