[en] Changes in the metabolic pattern of proline-¹⁴C were examined in barley seedlings germinated at the low temperature of 2⁰C(LT) and compared with those germinated at 25⁰C(HT). In the LT shoots, proline-¹⁴C incorporation was higher in the cationic fraction and lower in the acid-neutral fraction than that of HT, respectively. More proline-¹⁴C of LT was converted to other amino acids, especially to acidic amino acids in free amino acid state, than was the HT proline-¹⁴C which had a comparatively wide distribution. In the LT protein fractions, more proline-¹⁴C was incorporated into the cytoplasmic protein than into the cell wall protein. On the contrary, the radioactivity of the HT cell wall increased more distinctively. Proline-¹⁴C in the two proteins of LT was a little less converted to other amino acids than in those of HT. A little higher radioactivity was found in the aspartate and glutamate of the LT protein hydrolysates. The hydroxyproline which is closely related with proline had a little lower level of radioactivity in the LT cell wall. (auth.)