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[en] Under appropriate conditions the haemolytic activity of mouse complement can be measured in a 51Cr release assay. Modifications that increase the sensitivity are the following: (a) mouse 7 S anti-SRBC antibodies as amboceptor; (b) low amount of target cells; (c) ionic strength of 0.13 M NaCl in the test medium; (d) incubation temperature of 300C; (e) incubation time of 90 min; (f) total reaction volume of 1 ml. (Auth.)
Journal
Journal of Immunological Methods; ISSN 0022-1759; 
; v. 23(1-2); p. 149-159
; v. 23(1-2); p. 149-159