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AbstractAbstract
[en] The Zimm theory of rotor speed dependent DNA sedimentation has been tested quantitatively and found to be correct as predicted by the equation of Zimm and Schumaker. The quantitative validity of the theory means that the size of a DNA sedimenting under speed-dependent conditions is not undefinable but rather can be uniquely obtained by the application of that theory to the data. The viscoelastic recoil or more accurately, the zero shear rate reduced recoil has been shown to be a quantitative direct function of the number of intact DNA molecules present in solution. This demonstration made possible the measurement of a direct survival curve for intact DNA molecules after exposure to ionizing radiation. A D37 of 49.5 krads was obtained for the DNA of T4c coliphage irradiated as a solution of phage particles. It is noteworthy that this survival curve measures the number of intact DNA molecules, not the average number of breaks/molecule
Original Title
Quantitation of rotor speed dependent DNA sedimentation and zero shear rate reduced recoil
Primary Subject
Source
1979; 13 p; Available from NTIS., PC A02/MF A01
Record Type
Report
Literature Type
Progress Report
Report Number
Country of publication
BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, BIOLOGICAL EFFECTS, COBALT ISOTOPES, ELECTROMAGNETIC RADIATION, INTERMEDIATE MASS NUCLEI, IONIZING RADIATIONS, ISOMERIC TRANSITION ISOTOPES, ISOTOPES, MECHANICAL PROPERTIES, MICROORGANISMS, MINUTES LIVING RADIOISOTOPES, NUCLEI, NUCLEIC ACIDS, ODD-ODD NUCLEI, ORGANIC COMPOUNDS, PARASITES, RADIATION EFFECTS, RADIATIONS, RADIOISOTOPES, VELOCITY, VIRUSES, YEARS LIVING RADIOISOTOPES
Reference NumberReference Number
INIS VolumeINIS Volume
INIS IssueINIS Issue