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AbstractAbstract
[en] We investigated three aspects of the light driven sodium pump (halorhodopsin, which appear to be crucial to our understanding of the mechanisms employed by Halobacterium halobium and to further investigate this unique system of energy conservation. We characterized the molecular mechanisms of transmembrane sodium transport in vesicles from H. halobium with particular reference to the mechanism of couplins of light energy to net sodium translocation. We develop procedures and techniques for extracting the components of the light driven sodium pump from membranes and incorporating them into artificial membrane systems. We examine the mechanism of conversion of bacteriorhodopsin from an active to an inactive form in membrane vesicles and to relate this alternative state of this pigment to the presence of the light driven sodium pump
Primary Subject
Source
1982; 20 p; Available from NTIS, PC A02/MF A01; 1 as DE84005272
Record Type
Report
Literature Type
Progress Report
Report Number
Country of publication
ABSORPTION SPECTROSCOPY, ACTIVATION ANALYSIS, ASPARTIC ACID, ATP-ASE, BACTERIA, BIOCHEMICAL REACTION KINETICS, BIOLOGICAL FUNCTIONS, CYTOCHROMES, DIFFUSION, ELECTRON SPECTROSCOPY, GLUTAMIC ACID, LIPOSOMES, MEMBRANES, NEUTRON REACTIONS, PHOSPHOTRANSFERASES, PHOTOCHEMISTRY, PIGMENTS, RAMAN SPECTRA, SERINE, SODIUM, SODIUM 22, TRACER TECHNIQUES, VISIBLE RADIATION
ALKALI METALS, AMINO ACIDS, BARYON REACTIONS, BETA DECAY RADIOISOTOPES, BETA-PLUS DECAY RADIOISOTOPES, CARBOXYLIC ACIDS, CELL CONSTITUENTS, CHEMICAL ANALYSIS, CHEMISTRY, ELECTROMAGNETIC RADIATION, ELEMENTS, ENZYMES, ESTERASES, HADRON REACTIONS, HYDROLASES, HYDROXY ACIDS, ISOTOPE APPLICATIONS, ISOTOPES, KINETICS, LIGHT NUCLEI, METALS, MICROORGANISMS, NUCLEAR REACTIONS, NUCLEI, NUCLEON REACTIONS, ODD-ODD NUCLEI, ORGANIC ACIDS, ORGANIC COMPOUNDS, PHOSPHATASES, RADIATIONS, RADIOISOTOPES, REACTION KINETICS, SODIUM ISOTOPES, SPECTRA, SPECTROSCOPY, TRANSFERASES, YEARS LIVING RADIOISOTOPES
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