[en] The use of computers in conjunction with gamma-cameras has led to development of a quantitative method of determining the concentration of ¹³¹ I-labeled cholesterol in the adrenals. Several methods of subtracting the background during adrenal imaging have been suggested and used. This paper examines the need for the most appropriate method of determination of the background, allowing for the indeterminacy of the initial information. The method of averaging and the suggested method of determination of the background were used for comparison. It is shown that the suggested method of subtraction of the background is free from disadvantages since the resultant curve of radioactivity along the section is used, and this increases the accuracy of the method