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AbstractAbstract
[en] Purification of human prolactin from pituitaries was carried out in our laboratory to obtain a pure reagent for use in RIA. The extraction and purification procedure was adapted from the method of Mc. Lean et al., and it involves the following steps: 1. Extraction of frozen pituitaries in buffers 0.14M phosphate/citrate pH 4.0 and 0.05M ammonium acetate pH 10.0. 2. Purification by hydrophobic interaction chromatography on Phenyl-Sepharose CL-4B in the presence of acetonitrile. 3. Purification by anion exchange chromatography on DEAE-Sepharose Cl-68. The purification method is considered effective for obtaining a hPrl of the purity needed for radioassay purposes, having the advantage of rapidity and relative simplicity. (author)
[pt]
A purificacao de prolactina humana a partir de hipofises foi desenvolvida em nosso laboratorio com o objetivo de obtermos um reagente puro para uso em radioimunoensaio. O procedimento de extracao e purificacao foi adaptado do metodo de McLean et al., o qual envolve as seguintes etapas: 1. Extracao de hipofises congeladas em tampao fosfocitrato 0,14M, pH 4,0 e tampao acetato de amoneo 50 mM em pH 10,0. 2. Purificacao por cromatografia de interacao hidrofobica em Phenyl-Sepharose CL-4B na presenca de acetonitrila. 3. Purificacao por cromatografia de troca ionica em DEAE-Sepharose CL-6B. O metodo de purificacao foi considerado eficaz para obtencao de hPrl com a pureza necessaria para a preparacao de reagentes usados em radioimunoensaios, possuindo a vantagem de ser rapido e relativamente simples. (autor)Original Title
Miniextracao e purificacao da prolactina humana para preparacao de reagentes usados em radioimmunoensaio
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1989; 117 p; Tese (M.Sc.).
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Miscellaneous
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Thesis/Dissertation
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