[en] Bore holes open to large intervals provide groundwater samples and test results which represent an unknown integration of properties throughout the depth of the hole. The State of Idaho's INEL Oversight Program is utilizing a custom straddle-packer system to develop a vertical characterization of water chemistry and hydrology in selected open bore holes at the Idaho National Engineering Laboratory. This report describes the design and operation for bore hole zone isolation, water sampling, and hydrologic testing. To reduce potential influences on in situ water chemistry, the system utilizes chemically inert components to the extent possible. Straddle packer systems are effective in producing representative water samples from isolated formations. Hydrologic testing is limited by the ability to produce a measurable stress on the aquifer in individual formations, and head measurement sensitivity. 4 figs