Filters
Results 1 - 1 of 1
Results 1 - 1 of 1.
Search took: 0.013 seconds
[en] An indirect enzyme linked immunosorbent assay (ELISA) kit was used for diagnosis of foot-and-mouth disease virus (FMDV) types O1, A23, C3 which occurred in Rio Grande do Sul State, Southern Brazil during 1984-1994. The samples were randomly selected and tested by ELISA, Complement Fixation Test (CFT) and in tissue culture. Out of 106 samples 78 (73,5%) were positive by ELISA and 39 (36,8%) were found positive in CFT, when original suspensions were used. Once these samples were inoculated onto tissue culture both tests gave similar results, although ELISA picked up more positive samples during the 1st passage in tissue culture. The negative samples (16) included in this study were negative in all tests. The ELISA was more sensitive than and as specific as CFT. ELISA and tissue culture together were shown to be a better system for detection of foot-and-mouth disease virus antigen than CFT. (author)
Source
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria); 302 p; ISSN 1011-4289; 
; Nov 1998; p. 195-198; Final research co-ordination meeting on immunoassay methods for the diagnosis and epidemiology of animal diseases in Latin America; Guadeloupe, Lesser Antilles (France); 13-17 Jun 1994; 10 refs, 3 tabs
; Nov 1998; p. 195-198; Final research co-ordination meeting on immunoassay methods for the diagnosis and epidemiology of animal diseases in Latin America; Guadeloupe, Lesser Antilles (France); 13-17 Jun 1994; 10 refs, 3 tabs