[en] The Nuclear Microprobe (NMP) unit of the National Accelerator Centre (NAC) initiated a focused research programme on studies of biological material, ranging from applications in medicine, to agriculture and botany. During this period a state-of-the-art cryo-preparation laboratory was also developed. This research programme has resulted in a wide range of projects, and has shown how well-suited the NMP is for studies of biological material in general. Various research projects have been undertaken and the experimental setup has been optimised for biological research. This contribution will report on some of the problems and demands in this field, as well as some of the results obtained using PIXE and RBS simultaneously. Imaging is routinely performed using the GeoPIXE suite of programmes, with semi-quantitative Dynamic Mapping. The collaborative project on single-event studies of living cells, together with the CENBG group of Bordeaux-Gradignan in France will also be discussed, with emphasis on the state of the technology and the demands for delivering single MeV ions on single living cells at the resolution of a few micrometer. (author)