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[en] In vitro culture and irradiation techniques were used for obtaining mutants in sweet potato, lpomoea batatas. Callus was initiated from stem explants cultured on MS medium, supplemented with 1 mg NAA, 2 mg IAA, and 0.01 mg BA per litre. Calli were irradiated with 5 Gy gamma rays, and cultured on half-strength MS medium containing 2 mg IAA and 2 mg kinetin per litre. Variants for morphological changes in leaf and tuber skin and flesh color were found among the M1V2 plants, derived from irradiated calli. A promising clone, 91-C3-15, with improved tuber shape and deep red skin was selected from the 776 M1V2 population. (author)
Source
Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture, Vienna (Austria); 92 p; ISSN 1011-4289; 
; Jun 2001; p. 79-82; Final research co-ordination meeting on in vitro techniques for selection of radiation induced mutations adapted to adverse environmental conditions; Shanghai (China); 17-21 Aug 1998; 5 refs, 4 tabs
; Jun 2001; p. 79-82; Final research co-ordination meeting on in vitro techniques for selection of radiation induced mutations adapted to adverse environmental conditions; Shanghai (China); 17-21 Aug 1998; 5 refs, 4 tabs