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AbstractAbstract
[en] Objective: To evaluate the binding ability of LHRH-PE40, a fusion protein, to the LHRH receptors on cancer cell line. Methods: The radioligand binding assay of receptors was used to calculate the Kd and Bmax. Results: Hela cell line: Kd=(0.36 +- 0.12) nmol, Bmax=(0.23+-0.15) μmol·g-1; Hep2 cell line: Kd=(0.33 +- 0.11) nmol, Bmax=(0.46 +- 0.12)μmol·g-1. Conclusion: LHRH-PE40 has a high binding affinity to the LHRH receptors on cancer cell line, which is the same as the natural LHRH
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Journal Article
Journal
Journal of Jilin University. Medicine Edition; ISSN 1671-587X;
; v. 29(1); p. 5-8

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ANIMAL CELLS, BETA DECAY RADIOISOTOPES, BODY, DAYS LIVING RADIOISOTOPES, ELECTRON CAPTURE RADIOISOTOPES, FEMALE GENITALS, HORMONES, INTERMEDIATE MASS NUCLEI, INTERNAL CONVERSION RADIOISOTOPES, IODINE ISOTOPES, ISOTOPE APPLICATIONS, ISOTOPES, MEMBRANE PROTEINS, NUCLEI, ODD-EVEN NUCLEI, ORGANIC COMPOUNDS, ORGANS, PEPTIDE HORMONES, PITUITARY HORMONES, PROTEINS, RADIOASSAY, RADIOISOTOPES, TRACER TECHNIQUES, USES
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