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Breitfellner, F.; Solar, S.; Sontag, G.
10. 'Tihany' symposium on radiation chemistry. Program and abstracts2002
10. 'Tihany' symposium on radiation chemistry. Program and abstracts2002
AbstractAbstract
[en] Complete text of publication follows. The investigations were directed to the determination of the effect of γ-irradiation on various phenolic compounds in strawberries in dependence of dose. A significant decrease of these compounds during irradiation could reduce their beneficial effect on health, which are based on their antioxidative and anticarcinogenic properties. On the other hand hydroxilation of phenolic acids has been proposed as a promising method to distinguish between irradiated and not irradiated fruits and vegetables. Irradiated and not irradiated strawberry samples were homogenized, centrifuged and chromatographically purified from matrix components on polyamide columns. For determination of hydroxybenzoic and hydroxycinnamic acids, which are present as esters or as glycosides, the samples had to be acid/base hydrolized prior to purification. The individual compounds were separated by reversed phase chromatography and detected by means of a diode-array-detector. Peak identification was based on both UV-Vis-spectra and retention times compared with those of standards. In hydrolized samples four phenolic acids [gallic acid, 4-hydroxybenzoic acid, p-coumaric acid and caffeic acid] were identified. Only 4-hydroxybenzoic acid was affected by irradiation (build up with dose). Five flavonoids were detected in non hydrolized samples [(+)-catechin, (-)-epicatechin, kaempferol-3-glucoside, quercetin-3-glucoside and, in trace quantities, quercetin-3-galactoside], the concentration of the catechines and of kaempferol-3-glucoside decreased as irradiation dose increased, whereas those of quercetin-3-glucoside remained unchanged. In addition two as yet unclassified compounds showed a significant change of concentration upon irradiation. One of them (m/e = 450) is decreasing, one (m/e = 318) is increasing to the fivefold at a dose of 6 kGy
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Hungarian Academy of Sciences, Institute of Isotope and Surface Chemistry (Hungary); [140 p.]; 2002; p. P-39; 10. 'Tihany' symposium on radiation chemistry; Sopron (Hungary); 31 Aug - 5 Sep 2002
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Miscellaneous
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Conference
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AROMATICS, BERRIES, CARBOXYLIC ACIDS, CHEMICAL RADIATION EFFECTS, CHEMICAL REACTIONS, DECOMPOSITION, DOSES, ELECTROMAGNETIC RADIATION, FLAVENOIDS, FOOD, FRUITS, HYDROXY COMPOUNDS, IONIZING RADIATIONS, MAGNOLIOPHYTA, MAGNOLIOPSIDA, ORGANIC ACIDS, ORGANIC COMPOUNDS, ORGANIC OXYGEN COMPOUNDS, PLANTS, RADIATION EFFECTS, RADIATIONS, ROSACEAE
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