[en] In this investigation, the response of anther culture in three wheat genotypes (Tajan, Atila, R-12) after a treatment with a low dose of gamma ray was studied. Spikes of these genotypes were irradiated in doses of 2, 3 Gy, then cultured in the modified P⁴ medium, containing 200 mgl^-1 Glutamine, 15% Ficol, 2 mgl^-1 2,4-D, and 0.5 mgl^-1 Kinitin. It was found that there is a significant difference between the genotypes, and also between the irradiated treatment levels and the non-irradiated treatment. All of the genotypes showed a low response to the anther culture meaning a decrease in the response with irradiation. 90% of Calli of Atila, 67% of R-12 and 46% of Tajan in control treatment produced plants. But the Calli of irradiated anthers did not produced any plant (green or albino). Generally by the gamma irradiation, we observed a low response in the Calli and plant let production