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[en] Detection of hepatitis C virus in sera samples by specific amplification of the 5' non-coding conserved region of HCV. 8 anti-HCV EIAs positive blood donors sera were evaluated, 5 from the Hospital Nacional Cayetano Heredia (HNCH) and 3 from the Instituto de Enfermedades Neoplasicas (INEN). RNA was extracted from sera using trizol and reverse transcripted with primer 209. Nested PCR amplification was carried out with primers 209-939 in a first round and 211-940 primers during the second round of amplification. Visualization of amplification products was done after electrophoresis in 2% agarose gel and ethidium bromide staining
Source
Gayoso C, C.; Ezpinoza Z, M.; Prado C, A.; Robles N, A. (eds.); Instituto Peruano de Energia Nuclear (IPEN), Lima (Peru); 512 p; ISSN 1684-1662; 
; 2002; p. 200; Also available from the Library of the Peruvian Institute of Nuclear Energy in electronic format (pdf)
; 2002; p. 200; Also available from the Library of the Peruvian Institute of Nuclear Energy in electronic format (pdf)