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Optimization of the synthesis of a high specific activity 125I-labelled hapten for radioimmunoassays
Suraez, C.; Paz, D.; Simon, M. A.; Romero del Hombrebueno, B.
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas (CIEMAT), Madrid (Spain)1994
Centro de Investigaciones Energeticas Medioambientales y Tecnologicas (CIEMAT), Madrid (Spain)1994
AbstractAbstract
[en] In this first report it is described the synthesis, separation and purification of the 2-radioiodinated histamine- I-labelled histamine by a mixed anhydride reaction. About 75% incorporation of I-125, from Na125I, was achieved with a molecular ratio of 1:1 mixed anhydride:histamine. The radiochemical purity of the conjugate by TLC was > 99% and its theoretical specific activity, 3850 μCi/μg. Dissolved in ethanol and held at -20 degree centigree under darkness decomposition on storage didn't exceed 1% per month. (Author) 13 refs
Original Title
Optimizacion de un metodo de preparacion de un radiohapteno-125''I para ria de alta actividad especifica
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Source
1994; 29 p
Record Type
Report
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Country of publication
ALCOHOLS, BETA DECAY RADIOISOTOPES, BIOASSAY, CHEMISTRY, DAYS LIVING RADIOISOTOPES, DIAGNOSTIC TECHNIQUES, ELECTRON CAPTURE RADIOISOTOPES, HYDROXY COMPOUNDS, IMMUNOASSAY, INTERMEDIATE MASS NUCLEI, INTERNAL CONVERSION RADIOISOTOPES, IODINE ISOTOPES, ISOTOPE APPLICATIONS, ISOTOPES, NUCLEI, ODD-EVEN NUCLEI, ORGANIC COMPOUNDS, RADIOASSAY, RADIOIMMUNODETECTION, RADIOISOTOPES, TRACER TECHNIQUES
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