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Chapeville, F.; Fromageot, P.
CEA Saclay, 91 - Gif-sur-Yvette (France)1960
CEA Saclay, 91 - Gif-sur-Yvette (France)1960
AbstractAbstract
[en] Previous work has shown that the formation of cysteic acid from sulphate in incubated hen's eggs is due to the following reactions: a) reduction of sulphate to sulphite by the yolk sac endoderm cells; b) synthesis of cysteic acid from the sulphite in the presence of cysteine with liberation of hydrogen sulphide: HS-CH2-CH(NH2)-COOH + SO3H- → H2S + -O3S-CH2-CH(NH2)-COOH (1). The enzymatic system responsible for this reaction is localized on the yolk sac endoderm and in the yolk. It may be wondered whether reaction (1) is not made up of two consecutive reactions, one of which is reversible: HS-CH2-CH(NH2)-COOH ↔ H2S + organic chain (2) and organic chain + SO3H- → -O3S-CH2-CH(NH2)-COOH (3). It would then be clear why the addition of sulphite displaces the equilibrium towards the production of cysteic acid and hydrogen sulphide. If this is the case, the addition of ordinary cysteine and of marked hydrogen sulphide to the biological medium should make it possible to detect the formation of 35S cysteine. The present work shows that the desulphurization of the cysteine (reaction 2) by the yolk sac + the yolk is in fact a reversible reaction, and that an enzymatic exchange occurs between the sulphur of the cysteine and that of the hydrogen sulphide. (author)
[fr]
Un precedent travail a montre que la formation de l'acide cysteique a partir de sulfate dans l'oeuf embryonne de poule correspond aux reactions suivantes: a) Reduction du sulfate en sulfite par les cellules de l'endoderme du sac vitellin; b) synthese de l'acide cysteique a partir du sulfite en presence de cysteine avec liberation de l'hydrogene sulfure: HS-CH2-CH(NH2)-COOH + SO3H- → H2S + -O3S-CH2-CH(NH2)-COOH (1). Le systeme enzymatique responsable de cette reaction est localise dans l'endoderme du sac vitellin et dans le vitellus. On peut se demander si la reaction (1) n'est pas composee de deux reactions successives dont l'une serait reversible: HS-CH2-CH(NH2)-COOH ↔ H2S + chaine organique (2) and chaine organique + SO3H- → -O3S-CH2-CH(NH2)-COOH (3). On comprendrait des lors que l'addition du sulfite deplace l'equilibre vers la production d'acide cysteique et d'hydrogene sulfure. Si tel est le cas, en introduisant dans le milieu biologique de la cysteine ordinaire et de l'hydrogene sulfure marque, on doit pouvoir mettre en evidence la formation de cysteine 35S. Le present travail montre en effet que le desulfhydration de la cysteine (reaction 2) par le sac vitellin + vitellus est une reaction reversible et qu'il se produit un echange enzymatique entre le soufre de la cysteine et celui de l'hydrogene sulfure. (auteur)Original Title
Echange enzymatique entre le soufre de la cysteine et celui de l'hydrogene sulfure dans le sac vitellin de l'oeuf embryonne d'oiseau
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Source
1960; 11 p; 9 refs.
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Report
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ALDEHYDES, AMINO ACIDS, ANIMALS, AZINES, BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, CARBOXYLIC ACIDS, CHALCOGENIDES, CHEMICAL REACTIONS, CHEMISTRY, DAYS LIVING RADIOISOTOPES, DISULFIDES, EVEN-ODD NUCLEI, HETEROCYCLIC COMPOUNDS, HYDROGEN COMPOUNDS, ISOTOPE APPLICATIONS, ISOTOPES, LIGHT NUCLEI, NUCLEI, ORGANIC ACIDS, ORGANIC COMPOUNDS, ORGANIC NITROGEN COMPOUNDS, ORGANIC OXYGEN COMPOUNDS, ORGANIC SULFUR COMPOUNDS, PYRIDINES, RADIOISOTOPES, SULFIDES, SULFUR COMPOUNDS, SULFUR ISOTOPES, THIOLS, VERTEBRATES
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