[en] Reversed phase column (RPC) extraction chromatography is an useful technique and has been carried out successfully to the separation of various metals and organic compounds. Its application has received considerable attention because it combines the selectivity of LLE with the advantage of chromatography. The efficiency of the separation by RPC depends on both the chemical and physical properties of the solid support and the stationary phase. This work describes the preparation of an improved adsorbent from natural perlite and its properties to uptake of uranyl ion. Perlite is a volcanic glassy rock produced in commercially workable quantities from mines of Aegean region of Turkey and contains 70-75% CO₂ .The CO₂ was converted to soluble silicates with NaOH modification then its acidification to form hydrogen which turned into xerogels upon drying. Fundamental parameters (particle size, specific surface area, pore size and volume, surface hydroxyl group density) were determined for modified perlite and it was silanized then loaded with 20% (w/w) TBP before being used as reversed phase column chromatography solid support. Finally the sorption of UO²+₂ from aqueous solutions by the modified perlite was investigated using Batch techniques. The use of TBP-loaded perlite as a reversed phase column (RPC) extraction chromatography support seems to be useful to uptake of UO²+₂ from aqueous solutions and to separate from various cations