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[en] Modifications occurred in Escherichia coli cells exposed to gamma radiation (60Co source) were investigated. The irradiations were done at the LIN-COPPE laboratory of the UFRJ and the analysis at the Biology Department of the UTFPR. The E. coli cells were irradiated with 30, 60, 90, 120, 150, 180, 210, 240, 300, 480, 600 e 750 Gy doses. The samples were analyzed with Gram-stain, biochemical tests in EPM, MIO and Lysine Broth, Simmons Cytrate Medium and Rhamnose Broth, antibiogram and isolation of auxotrophic mutants. It was observed that for the received doses the E. coli did not show morphological alterations in the tests. Some E. Coli cells showed to be able to deaminade the L-tryptophan or they changed their sensibility for amoxillin and cephaloonine after the irradiation. The existence of aauxotrophic mutants after irradiation was also verified. (author)
Source
Associacao Brasileira de Energia Nuclear, Rio de Janeiro, RJ (Brazil); [vp.]; ISBN 978-85-99141-02-1; 
; 2007; [6 p.]; INAC 2007 International nuclear atlantic conference. Nuclear energy and energetic challenges for 21st. century. 15. Brazilian national meeting on reactor physics and thermal hydraulics; Santos (Brazil); 30 Sep - 5 Oct 2007; 8. Brazilian national meeting on nuclear applications; Santos (Brazil); 30 Sep - 5 Oct 2007; Available from the Library of the Brazilian National Nuclear Energy Commission, Rio de Janeiro, in electronic form; 11 refs., 4 figs., 1 tab. Code: E111708.pdf
; 2007; [6 p.]; INAC 2007 International nuclear atlantic conference. Nuclear energy and energetic challenges for 21st. century. 15. Brazilian national meeting on reactor physics and thermal hydraulics; Santos (Brazil); 30 Sep - 5 Oct 2007; 8. Brazilian national meeting on nuclear applications; Santos (Brazil); 30 Sep - 5 Oct 2007; Available from the Library of the Brazilian National Nuclear Energy Commission, Rio de Janeiro, in electronic form; 11 refs., 4 figs., 1 tab. Code: E111708.pdf