[en] The single crystal of ZnWO₄:Ho³⁺ was grown by Czochralski technique. The XRD, absorption spectra, fluorescence spectrum are presented, and the Judd-Ofelt (J-O) intensity parameters Ω₂, Ω₄, Ω₆ are obtained to be 4.61 x 10^-20 cm², 0.16 x 10^-20 cm², 0.48 x 10^-20 cm², respectively. Calculated radiative transition rate, branching ratios, and radiative lifetime for different transition levels of ZnWO₄:Ho³⁺crystals are presented. The most intense line correlative with the transition ⁵S₂ → ⁵I₈ at 543 nm is potentially used for green solid state laser, and the fluorescence lifetime of this energy level is 14.1 μs