[en] We demonstrate an asymmetric optical potential barrier for ultracold ⁸⁷Rb atoms using laser light tuned near the D₂ optical transition. Such a one-way barrier, where atoms incident on one side are transmitted but reflected from the other, is a realization of Maxwell's demon and has important implications for cooling atoms and molecules not amenable to standard laser-cooling techniques. In our experiment, atoms are confined to a far-detuned dipole trap consisting of a single focused Gaussian beam, which is divided near the focus by the barrier. The one-way barrier consists of two focused laser beams oriented almost normal to the dipole-trap axis. The first beam is tuned to present either a potential well or barrier, depending on the state of the incident atoms. On the reflecting side of the barrier, the second beam optically pumps the atoms to the reflecting (barrier) state, thus producing the asymmetry