[en] In the production process of health care products, contamination must be considered as one of the principal hazards to be avoided. Among the developed methods for sterilization, ionizing radiation has largely been used by many sectors in health care area as it is efficient in eliminating biological contaminants of several origins. The difficulty of deploying ionizing radiation in materials of human origin, though, includes which possible alterations it might cause in human tissue. In the present work, the extension of the bio mechanical alteration generated by radiation in bone tissue was evaluated by bio mechanical methods. More specifically, we evaluated alterations to the elastic modulus, rupture tension and percentage of deformation that are thought to be a consequence of the sterilization process. As a research model, bovine femur struts obtained from the diaphysis were used. The struts were frozen in a temperature of -70 deg C and irradiated with crescent doses of gamma radiation (0, 12.5, 25 e 50 kGy). During this work, a cutting system to obtain precision samples to use in such essays was developed. As results show that there is a significant different between the analyzed characteristics in the different doses of radiation. (author)