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AbstractAbstract
[en] Tissue Banks around the world have stored human cartilages obtained from post mortem donors for use in several kinds of reconstructive surgeries. To ensure that such tissues are not contaminated, they have been sterilized with ionizing radiation. However, high doses of gamma radiation may cause undesirable changes in the tissues. In this work, we evaluated the possibility of use Optical Coherence Tomography (OCT) and Thermogravimetric Analysis (TGA) to identify possible structural modifications caused by both preservation methods of cartilage and gamma irradiation doses. Cartilages were obtained from cadaveric donors and were frozen at -70 deg C or preserved in glycerol. Irradiation was performed by 60Co source with doses of 15, 25 and 50 kGy. Our TGA results showed that glycerolized cartilages irradiated with different doses of radiation does not presented statistical differences when compared to the control group for the dehydration rate. However, the same was not observed for deep-frozen cartilages irradiated with 15 kGy. The results of OCT associated to total optical attenuation coefficient showed that doses of 15 kGy promote cross-link between collagen fibrils, corroborating the results obtained from TGA. Moreover, total optical attenuation coefficient values are proportional to stress at break of cartilages, what will be very useful in a near future to predict the quality of the allografts, without unnecessary loss of biological tissue, once OCT is a nondestructive technique. By PS-OCT images, we found that high doses of ionizing radiation does not promote sufficient impairments to promote complete loss of tissue birefringence. Thus, TGA and OCT are techniques that can be used for tissue banks to verify tissue quality before its transplant. (author)
Original Title
Estudo dos efeitos da radiacao ionizante em cartilagem costal humana por meio de termogravimetria e tomografia por coerencia optica
Primary Subject
Source
2012; 240 p; Thesis (Ph.D.)
Record Type
Miscellaneous
Literature Type
Thesis/Dissertation
Report Number
Country of publication
ANIMAL TISSUES, BETA DECAY RADIOISOTOPES, BETA-MINUS DECAY RADIOISOTOPES, BIOLOGICAL EFFECTS, BODY, CHEMICAL ANALYSIS, COBALT ISOTOPES, CONNECTIVE TISSUE, DIAGNOSTIC TECHNIQUES, DOSES, ELECTROMAGNETIC RADIATION, GRAVIMETRIC ANALYSIS, INTERMEDIATE MASS NUCLEI, INTERNAL CONVERSION RADIOISOTOPES, IONIZING RADIATIONS, IRRADIATION, ISOMERIC TRANSITION ISOTOPES, ISOTOPES, MEDICINE, MINUTES LIVING RADIOISOTOPES, NUCLEI, ODD-ODD NUCLEI, QUANTITATIVE CHEMICAL ANALYSIS, RADIATION EFFECTS, RADIATIONS, RADIOISOTOPES, STERILIZATION, THERMAL ANALYSIS, YEARS LIVING RADIOISOTOPES
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