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[en] In the Sterile Insect Technique (SIT) programme a large number of homogenous age of pupae should be produced to be sterilized and released into the field. A methodology to preserve eggs which at the same time delay the egg hatch is needed in the production of a large number of pupae with homogenous age. The study on method of preserving fruit fly eggs the effect of dipping, low temperature, on the viability of eggs, the quality of larvae hatched from the eggs, and the quality of pupae produced were observed. Viability of eggs were observed as percent of egg hatch, quality of larvae as number of pupae produced from 0.5 ml of eggs when reared on artificial diet, and quality of pupae as percent of the flies number emerged and flew from the pupae. In room temperature (26 oC) dipping did not preserve fruit fly eggs and aeration did not improve the preservation. Although after one day dipping the viability of eggs only decreased from 90 % (control) to 80.5 %, (without aeration) and 81.5 % (with aeration) it decreased the number of pupae been produced from 1447 pupae (control) to 382 pupae (without aeration) and 616 pupae (with aeration). Low temperature successfully preserved the egg up to 24 hours. After 24 hour dipping in low temperature (16 oC) the viability of eggs were decreased up to 84.75 % (without aeration) and able to produce 1104 pupae. Aeration during dipping in low temperature did not improve the preservation. (author)
Source
Singgih Sutrisno; Sofyan Yatim; Pattiradjawane, EIsje L.; Ismachin, Moch; Mugiono; Marga Utama (National Nuclear Energy Agency, Jakarta (Indonesia)) (eds.); Nelly Dhevita Leswara; Umar Mansur (University of Indonesia, Jakarta (Indonesia)) (eds.); Syamsul Arifin Achmad (Bandung Institute of Technology, Bandung (Indonesia)); Komaruddin Idris (Bogor Agricultural University, Bogor (Indonesia)) (and others); Centre for Research and Development of Isotopes and Radiation Technology, National Nuclear Energy Agency (Indonesia); 323 p; ISBN 979-3558-03-2; 
; Feb 2004; p. 123-129; The Scientific Meeting on Research and Development of Isotopes Application and Radiation; Seminar Ilmiah Penelitian dan Pengembangan Aplikasi Isotop dan Radiasi; Jakarta (Indonesia); 17-18 Feb 2004; Also available from Center for Development of Nuclear Informatics, National Nuclear Energy Agency, Puspiptek Area, Fax. 62-21-7560923, PO BOX 4274, Jakarta (ID); 8 refs.; 6 figs.
; Feb 2004; p. 123-129; The Scientific Meeting on Research and Development of Isotopes Application and Radiation; Seminar Ilmiah Penelitian dan Pengembangan Aplikasi Isotop dan Radiasi; Jakarta (Indonesia); 17-18 Feb 2004; Also available from Center for Development of Nuclear Informatics, National Nuclear Energy Agency, Puspiptek Area, Fax. 62-21-7560923, PO BOX 4274, Jakarta (ID); 8 refs.; 6 figs.