[en] In this study, a high-sensitive protein chip and kit for bioscience using the radioisotope (RI) detection technique have developed. The biochip for the detection of phosphorylation using a RI facilitates the detection of phosphorylation with minimum amount of a sample by simple processes, compared with the conventional method using an antibody (eg. ELISA method). Moreover, it showed extremely high sensitivity (more than 100 times detection sensitivity compared to conventional fluorescence detection technique). A multi-channel type Lab-on-a-chip (LOC) using MEMS (MicroElectro-Mechanical System) process based on the RI detection technique has also developed. It can be a useful tool for protein-protein interaction study and high-throughput screening (HTS) system in biological and medical research field. In addition, two types of Nano-carrier (Ag-PANI, SiNP) were prepared and demonstrated the feasibility of the RI carrier through micro-SPECT/CT imaging study. Finally, to develop a biocompatible RI carrier, 14 novel ligands for 99mTc-tricarbonyl complex were synthesized. Among them, a ligand which has great radiochemical purity (>98%) has been discovered. Based on the achieved results, further investigation toward to imaging agent which is able to accumulate into specific lesions is also needed