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[en] A brief account of activities carried out by the National Reference Centres (NRC) established on the basis of regional public health authorities of the Slovak Republic and the Office of Public Health of the Slovak Republic. (UVZ SR) in 2012 is presented. These activities are reported under the headings: (1) NRC for flu; (2) NRC for arbovirus and hemorrhagic fever; (3) NRC for poliomyelitis; (4) NRC for meningococci; (5) NRC to monitor the resistance of microorganisms to antibiotics; (6) NRC for morbilli, rubella and parotitis; (7) NRC for salmonellosis; (8) NRC for evaluation of late effects of chemicals by methods of genetic toxicology; (9) NRC for hydrobiology; (10) NRC for ecotoxicology; (11) NRC for non-ionizing radiation; (12) NRC for hydrothermal microclimate; (13) NRC tests for exposure of xenobiotics; (14) NRC for environmental microbiology; (15) NRC for Legionella in the environment; (16) NRC on pesticide residues; (17) NRC for impact assessment of ambient air pollution and an enclosed non-productive nature of the health of the population; (18) NRC for toxoplasmosis; (19) NRC Assessment of personal exposure and health risk; (20) NRC for pertussis and parapertussis; (21) NRC for pneumococcal disease; (22) NRC for syphilis; (23) NRC for hygienic drinking water problems; (24) NRC for listeriosis; (25) The National Information Centre for bacteriological (biological) and toxin weapons; (26) NRC for intestinal parasites; (27) NRC for diphtheria; (28) NRC for coagulase-positive staphylococci and their toxins; (29) NRC for Vibrionaceae; (30) NRC for environmental mycology; (31) NRC for noise and vibration; (32) NRC for articles of daily use and packaging materials; (33) NRC for thermotolerant amoebae; (34) NRC issues for coal mines; (35) NRC to work physiology and ergonomics; (36) NRC for health issues fibrous dusts; (37) NRC for haemophilus infection; (38) NRC for cosmetic products.